The Effect of Acute Beetroot Juice Supplementation on Anaerobic Performance in Football Players

NCT ID: NCT07048912

Last Updated: 2025-07-03

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 16 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2024-07-01
• Study Completion Date: 2024-07-25

Brief Summary

The aim of this clinical trial is to examine the effects of two different natural fruit-vegetable blend beverages on short-duration, high-intensity exercise performance and muscle oxygenation in young male football players. The study also investigates how these beverages influence certain metabolic responses. The main questions it seeks to answer are:

• Does one of the beverages improve peak and mean power output during the Wingate test?
• How do these beverages affect muscle oxygenation and post-exercise recovery?
• Researchers will compare the effects of the two beverages on performance and metabolic markers.

Participants:

• Consume one of the two natural beverages 2.5 hours before testing (assigned at random)
• Perform a 30-second high-intensity Wingate cycling test
• Undergo measurements of muscle oxygenation, heart rate, blood pressure, blood lactate, and blood glucose during and after exercise
• Maintain consistent dietary and physical activity routines and avoid certain foods prior to testing

Conditions

Exercise Performance; Supplementation

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: OTHER
• Blinding Strategy: TRIPLE

Study Groups

Beetroot Juice (BJ)

Participants in this arm consumed 140 mL of nitrate-rich beetroot juice (\~12.8 mmol nitrate) as a single acute dose, approximately 2.5 hours before the performance assessments. The supplementation was administered in the morning under supervision, and participants were instructed to maintain their usual diet but avoid additional nitrate-rich foods 24 hours prior to testing.

Group Type: EXPERIMENTAL

Beetroot Juice (BJ)

Intervention Type: DIETARY_SUPPLEMENT

This intervention involves a single 140 mL dose of a commercially available concentrated beetroot juice supplement (Beet-It Pro Elite Shot, James White Drinks Ltd., UK), containing approximately 12.8 mmol (800 mg) of dietary nitrate. Participants consume the juice 2.5 hours prior to performing a 30-second Wingate anaerobic test. The purpose of this intervention is to examine its acute effects on anaerobic performance, muscle oxygenation, and metabolic responses.

Blackcurrant Juice (Placebo)

Intervention Type: DIETARY_SUPPLEMENT

This intervention involves a single 140 mL dose of a low-calorie blackcurrant juice (Pfanner, Austria) with negligible nitrate content (\<0.1 mmol). The placebo is matched to beetroot juice in appearance, taste, and packaging to ensure double-blind conditions. It is consumed 2.5 hours before the Wingate test and serves as the control condition.

Placebo

Participants in this arm consumed 140 mL of blackcurrant juice containing negligible nitrate (\<0.1 mmol) as a single acute dose, approximately 2.5 hours before the performance assessments. The supplementation was administered in the morning under supervision, and participants followed the same dietary instructions as the experimental group, avoiding nitrate-rich foods 24 hours prior to testing.

Group Type: EXPERIMENTAL

Beetroot Juice (BJ)

Intervention Type: DIETARY_SUPPLEMENT

This intervention involves a single 140 mL dose of a commercially available concentrated beetroot juice supplement (Beet-It Pro Elite Shot, James White Drinks Ltd., UK), containing approximately 12.8 mmol (800 mg) of dietary nitrate. Participants consume the juice 2.5 hours prior to performing a 30-second Wingate anaerobic test. The purpose of this intervention is to examine its acute effects on anaerobic performance, muscle oxygenation, and metabolic responses.

Blackcurrant Juice (Placebo)

Intervention Type: DIETARY_SUPPLEMENT

This intervention involves a single 140 mL dose of a low-calorie blackcurrant juice (Pfanner, Austria) with negligible nitrate content (\<0.1 mmol). The placebo is matched to beetroot juice in appearance, taste, and packaging to ensure double-blind conditions. It is consumed 2.5 hours before the Wingate test and serves as the control condition.

Interventions

Beetroot Juice (BJ)

This intervention involves a single 140 mL dose of a commercially available concentrated beetroot juice supplement (Beet-It Pro Elite Shot, James White Drinks Ltd., UK), containing approximately 12.8 mmol (800 mg) of dietary nitrate. Participants consume the juice 2.5 hours prior to performing a 30-second Wingate anaerobic test. The purpose of this intervention is to examine its acute effects on anaerobic performance, muscle oxygenation, and metabolic responses.

Intervention Type: DIETARY_SUPPLEMENT

Blackcurrant Juice (Placebo)

This intervention involves a single 140 mL dose of a low-calorie blackcurrant juice (Pfanner, Austria) with negligible nitrate content (\<0.1 mmol). The placebo is matched to beetroot juice in appearance, taste, and packaging to ensure double-blind conditions. It is consumed 2.5 hours before the Wingate test and serves as the control condition.

Intervention Type: DIETARY_SUPPLEMENT

Eligibility Criteria

Inclusion Criteria

• Healthy males over 18 years old
• Performing resistance exercise at least three times per week
• At least 5 years of football training experience

Exclusion Criteria

• Contraindications to Wingate exercise
• Presence of cardiometabolic disease
• Current use of ergogenic supplementation
• Smokers

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 19 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

Melike Nur Eroğlu

OTHER

Sponsor Role: lead

Responsible Party

Melike Nur Eroğlu

Lecturer

Responsibility Role: SPONSOR_INVESTIGATOR

Locations

Sakarya University of Applied Sciences, Faculty of Sport Sciences, Exercise and Sports Sciences Education, Application and Research Centre

Sakarya, , Turkey (Türkiye)

Countries

Turkey (Türkiye)

Provided Documents

Document Type: Informed Consent Form

View Document

Other Identifiers

SUBU-EROGLU-2025-01

Identifier Type: -

Identifier Source: org_study_id