Acute Effects of Beetroot Juice and Caffeine Co-ingestion on Lower-limb Strength and Muscular Endurance in Females

NCT ID: NCT06758323

Last Updated: 2025-01-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

15 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-08-01

Study Completion Date

2024-12-26

Brief Summary

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The aim of this study is to evaluate the acute effects of beetroot juice and caffeine, consumed individually or in combination, on lower-limb strength and muscular endurance in physically active women. The main questions it aims to answer are:

* Does the co-ingestion of beetroot juice and caffeine enhance lower-limb strength and muscular endurance more than either substance alone or a placebo?
* How do the acute effects of beetroot juice interact with caffeine on physical performance?

The study was conducted using a randomized, crossover design to evaluate the following four conditions:

Beetroot juice with placebo Caffeine with placebo Beetroot juice with caffeine Placebo only

Each participant completed all conditions, with a sufficient washout period between sessions to eliminate potential carryover effects. This design allowed for a direct comparison of the acute effects of each supplementation strategy on lower-limb strength and muscular endurance.

Participant Protocol

Participants consumed their assigned supplement on a single testing day for each condition. After supplementation, they performed standardized tests to measure lower-limb strength and muscular endurance. Progress was monitored, and data from all conditions were collected to assess the acute effects of each intervention.

Detailed Description

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Conditions

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Beetroot Juice Caffeine Placebo Beetroot Juice with Caffeine Co-ingestion

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Double-Blind Crossover Randomized Trial
Primary Study Purpose

OTHER

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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COM

Beetroot juice combined with caffeine

Group Type EXPERIMENTAL

Beetroot juice combined with caffeine (COM)

Intervention Type DIETARY_SUPPLEMENT

* 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session
* 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session

CAF

Beetroot juice placebo with caffeine

Group Type ACTIVE_COMPARATOR

Beetroot juice placebo with caffeine (CAF)

Intervention Type DIETARY_SUPPLEMENT

\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session

BRJ

Beetroot juice with caffeine placebo

Group Type ACTIVE_COMPARATOR

Beetroot juice with caffeine placebo (BRJ)

Intervention Type DIETARY_SUPPLEMENT

\- 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session

PLA

Beetroot juice placebo with caffeine placebo

Group Type PLACEBO_COMPARATOR

Beetroot placebo with caffeine placebo (PLA)

Intervention Type OTHER

\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session

Interventions

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Beetroot juice combined with caffeine (COM)

* 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session
* 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session

Intervention Type DIETARY_SUPPLEMENT

Beetroot juice placebo with caffeine (CAF)

\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session

Intervention Type DIETARY_SUPPLEMENT

Beetroot juice with caffeine placebo (BRJ)

\- 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session

Intervention Type DIETARY_SUPPLEMENT

Beetroot placebo with caffeine placebo (PLA)

\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Minimal experience of six months of strength training
* Be familiarized with the exercise test: counter movement jump (CMJ), barbell back squats, leg press and knee extension.

Exclusion Criteria

* Supplementation consumption
* Sports injuries
Minimum Eligible Age

18 Years

Maximum Eligible Age

35 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Jose Manuel Jurado Castro

OTHER

Sponsor Role lead

Responsible Party

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Jose Manuel Jurado Castro

Principal Investigator

Responsibility Role SPONSOR_INVESTIGATOR

Locations

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Edificio IMIBIC Avda Menéndez Pidal s/n

Córdoba, , Spain

Site Status

Countries

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Spain

Other Identifiers

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IMIBIC_BRJ_CAF

Identifier Type: -

Identifier Source: org_study_id

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