Does Passive Movement Increase Glucose Uptake Into Muscle?

NCT ID: NCT06704126

Last Updated: 2025-08-11

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 11 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2024-12-01
• Study Completion Date: 2025-07-11

Brief Summary

This study aims to examine the blood sugar response after a meal, and how three different movement protocols may impact the blood sugar level.

Participants will visit the lab on three different occasions. Before each visit the participant will eat a meal we give them and have a cannula (a needle with a tube) put in their arm to allow blood sampling for the visit.

Visit A will be the participant just having their legs moved by the machine, visit B will be the participant having their legs moved by the machine with the addition of blood pressure cuffs on their thighs, and visit C will just be the participant sitting still on the machine.

Detailed Description

This study will require nine participants to be recruited and enrolled. The aim of the study is to determine the effect of passive movement, with and without blood flow restriction, on blood glucose and insulin primarily. Other outcomes included elsewhere in this registration. The protocol will involve an initial health screening/familiarisation session in the Human Performance Laboratory (HPL) at Lancaster University, followed by three randomly ordered HPL visits per participant. At the beginning of each HPL visit, the participant will be given a standard meal. The design is a crossover study therefore each participant will complete visits A, B and C and act as their own control.

Each of the visits A, B and C will involve consumption of a meal (86g carbohydrate content) and insertion of a cannula to the arm. Blood samples will then be drawn from the cannula at five minute intervals for approximately 3.5 hours. Three gold-top vacutainers will be taken throughout each visit to allow for insulin analysis. Once the blood is sampled, the vacutainer will be left to clot at room temperature for 15 minutes and then spun in a centrifuge at 4°C, 1800 RCF, for 10 minutes. The supernatant will be transferred to a microfuge tube via pipetting and stored at -80°C until analysis at a future date using an enzyme-linked immunosorbent assay (ELISA). In addition, 1ml syringe blood samples will be taken every 5 minutes and ejected onto a non-absorbent pad. A fraction of this ejection on the pad will then drawn up into a capillary tube to be analysed by a Biosen benchtop glucose/lactate analyser.

Visit A will invole the participant sitting on a Biodex isokinetic dynamometer and having both legs passively flexed and extended through 90 degrees, at a frequency of 1Hz for 30 minutes. This will be 60 seconds on, followed by 60 seconds off, repeated 15 times.

Visit B will involve the same as visit A, but with the addition of blood flow restriction intermittently. These are blood pressure bands (VALD AirBands) around the thighs which will inflate and deflate to cause 80% arterial occlusion in their inflated state. These will inflate and deflate in tandem with the passive movement going on and off.

Visit C is a control visit and will involve the participant sitting in the Biodex only with no movement or blood flow restriction occuring.

Throughout the study, measurements will be taken to meausure the outcome measure specified in this registration.

Conditions

Glucose; Insulin; Blood Flow Restriction Therapy; Blood Flow Velocity

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: PREVENTION
• Blinding Strategy: NONE

Study Groups

Passive movement only

Participants undergo passive movement training only

Group Type: EXPERIMENTAL

Passive movement

Intervention Type: OTHER

This study will use a passive movement protocol delivered on a Biodex isokinetic dynamometer at 1Hz for 60 seconds on, 60 seconds off, for a total of 30 minutes.

Passive movement + intermittent blood flow restriction

Participants undergo passive movement training with blood flow restriction applied intermittently during the passive movement protocol

Group Type: EXPERIMENTAL

Passive movement with blood flow restriction

Intervention Type: OTHER

This study will use a passive movement protocol delivered on a Biodex isokinetic dynamometer at 1Hz for 60 seconds on, 60 seconds off, for a total of 30 minutes. During the 60 seconds on of passive movement training, blood flow restriction cuffs which will be applied to the legs, will be inflated to cause 80% arterial blood flow occlusion.

Control

Sitting still in Biodex only - no movement

Group Type: NO_INTERVENTION

No interventions assigned to this group

Interventions

Passive movement

This study will use a passive movement protocol delivered on a Biodex isokinetic dynamometer at 1Hz for 60 seconds on, 60 seconds off, for a total of 30 minutes.

Intervention Type: OTHER

Passive movement with blood flow restriction

This study will use a passive movement protocol delivered on a Biodex isokinetic dynamometer at 1Hz for 60 seconds on, 60 seconds off, for a total of 30 minutes. During the 60 seconds on of passive movement training, blood flow restriction cuffs which will be applied to the legs, will be inflated to cause 80% arterial blood flow occlusion.

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• 18-35 years old, inclusive
• Male
• Healthy (no known long-term health conditions / acute febrile illness)

Exclusion Criteria

• Smokers
• Recent blood donation (within 12 weeks)
• Any medication
• Phobia of needles
• Blood pressure ≥ 140/90 mmHg, either figure
• Any potential atrial fibrillation or cardiovascular health conditions
• Diabetes
• Allergies to barley, gluten, nuts, almonds

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 35 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

Lancaster University

OTHER

Sponsor Role: lead

Responsible Party

Paul Hendrickse

Lecturer in Biomedicine

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Paul Hendrickse, PhD

Role: PRINCIPAL_INVESTIGATOR

Lancaster University

Locations

Lancaster University

Lancaster, , United Kingdom

Countries

United Kingdom

Other Identifiers

FHM-2024-4434-SA-2

Identifier Type: -

Identifier Source: org_study_id