Spirulina Platensis Supplementation and Calorie Restriction in Obese Men : A Randomized Controlled Trial Protocol Study

NCT ID: NCT06076161

Last Updated: 2024-02-29

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

32 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-10-17

Study Completion Date

2023-11-17

Brief Summary

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The goal of this clinical trial is to evaluate the effects of spirulina platensis supplementation and calorie restriction on anthropometric parameters, body composition, lipid profile, insulin resistance, serum superoxide dismutase (SOD) levels, and serum malondialdehyde (MDA) levels in men with obesity. The main question it aims to answer are:

What is the effect of spirulina platensis supplementation and calorie restriction on anthropometric parameters, body composition, lipid profile, insulin resistance, serum Superoxide Dismutase levels, and serum Malondialdehyde levels in obese individuals?

Participants will be respondents were given spirulina supplements for 30 days with a dose of 2x2 capsules 450mg

Researchers will compare intervention group with placebo group that weren't given spirulina supplementation to see if there is any effects on anthropometric parameters, body composition, lipid profile, insulin resistance, serum Superoxide Dismutase levels, and serum Malondialdehyde levels

Detailed Description

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Spirulina supplement intervention on subjects with male gender aged 25-55 years, which will be intervened for 4 weeks / 30 days, before the intervention will be taken anthropometric parameters, body composition, lipid profile, insulin resistance, serum Superoxide Dismutase levels, and serum Malondialdehyde levels.

After that, spirulina supplement intervention will be given at a dose of 2x2 / day, and follow-up will be carried out every week, after 30 hours of intervention,spirulina supplement distribution is carried out by research assistants every 2 days.

Will be taken again to conduct anthropometric parameters, body composition, lipid profile, insulin resistance, serum Superoxide Dismutase levels, and serum Malondialdehyde levels.

The aim is to find out if there is an effect of spirulina supplementation on anthropometric parameters, body composition, lipid profile, insulin resistance, serum Superoxide Dismutase levels, and serum Malondialdehyde levels.

Conditions

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Obesity

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

The number of respondents in this study amounted to 32 people. Respondents will be divided into 2 groups, namely the intervention group and the control group. The intervention group will be given spirulina platensis supplements at a dose of 2x2 /hr with a dose per capsule of 450 mg for 4 weeks with education about calorie restriction. The control group will be given placebo 2x2/day for 4 weeks with education on calorie restriction.
Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors
This study will use a randomized controlled trial triple blind study design with a pre-post design.

Study Groups

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Spirulina supplementation and calorie restriction in obese men

Respondents will be divided into 2 groups, namely the intervention group and the control group. The intervention group will be given spirulina platensis supplements at a dose of 2x2 /hr with a dose per capsule of 450 mg for 4 weeks with education about calorie restriction.

Group Type EXPERIMENTAL

Spirulina supplementation and calorie restriction in obese men

Intervention Type DIETARY_SUPPLEMENT

The intervention group will be given spirulina platensis supplements at a dose of 2x2 /hr with a dose per capsule of 450 mg for 4 weeks with education about calorie restriction. The control group will be given placebo 2x2/day for 4 weeks with education on calorie restriction.

Placebo capsules and calorie restriction in obese men

The control group will be given placebo 2x2/day for 4 weeks with education on calorie restriction.

Group Type PLACEBO_COMPARATOR

Spirulina supplementation and calorie restriction in obese men

Intervention Type DIETARY_SUPPLEMENT

The intervention group will be given spirulina platensis supplements at a dose of 2x2 /hr with a dose per capsule of 450 mg for 4 weeks with education about calorie restriction. The control group will be given placebo 2x2/day for 4 weeks with education on calorie restriction.

Interventions

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Spirulina supplementation and calorie restriction in obese men

The intervention group will be given spirulina platensis supplements at a dose of 2x2 /hr with a dose per capsule of 450 mg for 4 weeks with education about calorie restriction. The control group will be given placebo 2x2/day for 4 weeks with education on calorie restriction.

Intervention Type DIETARY_SUPPLEMENT

Other Intervention Names

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Placebo capsules

Eligibility Criteria

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Inclusion Criteria

* Male Age 25 - 55 years
* BMI ≥ 30 kg/m2
* Waist Circumference \> 90 cm
* Low physical activity (Sedentary life style)
* If taking weight loss supplements are willing to stop the dietary supplements, food or drinks.
* Not undergoing a diet program.

Exclusion Criteria

* History of kidney disease, atherosclerosis, cancer, and acute infections.
* Post-surgery in the last 3 months.
* Deficit in daily calorie requirement.
* Smoking habit and alcohol dependence.
* Use of hormonal drugs, antidepressant drugs, antibiotics, antidiuretic, glucocorticoids and use of vitamin or mineral supplements in the last three months.
* Refusal to sign the informed consent and in the study if not completing all stages will be excluded.
Minimum Eligible Age

25 Years

Maximum Eligible Age

55 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Universitas Diponegoro

OTHER

Sponsor Role lead

Responsible Party

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Adriyan Pramono

Lecturer

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Adriyan Pramono, PhD

Role: PRINCIPAL_INVESTIGATOR

Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Locations

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Central Laboratory, UNDIP Academic Hospital (Rumah Sakit Nasional Diponegoro)

Semarang, Central Java, Indonesia

Site Status

Countries

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Indonesia

References

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Fernandez-Sanchez A, Madrigal-Santillan E, Bautista M, Esquivel-Soto J, Morales-Gonzalez A, Esquivel-Chirino C, Durante-Montiel I, Sanchez-Rivera G, Valadez-Vega C, Morales-Gonzalez JA. Inflammation, oxidative stress, and obesity. Int J Mol Sci. 2011;12(5):3117-32. doi: 10.3390/ijms12053117. Epub 2011 May 13.

Reference Type BACKGROUND
PMID: 21686173 (View on PubMed)

Bluher M. Obesity: global epidemiology and pathogenesis. Nat Rev Endocrinol. 2019 May;15(5):288-298. doi: 10.1038/s41574-019-0176-8.

Reference Type BACKGROUND
PMID: 30814686 (View on PubMed)

Dludla PV, Nkambule BB, Jack B, Mkandla Z, Mutize T, Silvestri S, Orlando P, Tiano L, Louw J, Mazibuko-Mbeje SE. Inflammation and Oxidative Stress in an Obese State and the Protective Effects of Gallic Acid. Nutrients. 2018 Dec 21;11(1):23. doi: 10.3390/nu11010023.

Reference Type BACKGROUND
PMID: 30577684 (View on PubMed)

James WP. WHO recognition of the global obesity epidemic. Int J Obes (Lond). 2008 Dec;32 Suppl 7:S120-6. doi: 10.1038/ijo.2008.247.

Reference Type BACKGROUND
PMID: 19136980 (View on PubMed)

Chooi YC, Ding C, Magkos F. The epidemiology of obesity. Metabolism. 2019 Mar;92:6-10. doi: 10.1016/j.metabol.2018.09.005. Epub 2018 Sep 22.

Reference Type BACKGROUND
PMID: 30253139 (View on PubMed)

Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem. 1997 Jul;43(7):1209-14.

Reference Type RESULT
PMID: 9216458 (View on PubMed)

Rezaei N, Eftekhari MH, Tanideh N, Mokhtari M, Bagheri Z. Comparison of Antioxidant and Anti-Inflammatory Effects of Honey and Spirulina platensis with Sulfasalazine and Mesalazine on Acetic Acid-Induced Ulcerative Colitis in Rats. Galen Med J. 2019 Jun 10;8:e1095. doi: 10.31661/gmj.v8i0.1095. eCollection 2019.

Reference Type RESULT
PMID: 34466462 (View on PubMed)

Zarezadeh M, Faghfouri AH, Radkhah N, Foroumandi E, Khorshidi M, Rasouli A, Zarei M, Mohammadzadeh Honarvar N, Hazhir Karzar N, Ebrahimi Mamaghani M. Spirulina supplementation and anthropometric indices: A systematic review and meta-analysis of controlled clinical trials. Phytother Res. 2021 Feb;35(2):577-586. doi: 10.1002/ptr.6834. Epub 2020 Sep 23.

Reference Type RESULT
PMID: 32967062 (View on PubMed)

Naeini F, Zarezadeh M, Mohiti S, Tutunchi H, Ebrahimi Mamaghani M, Ostadrahimi A. Spirulina supplementation as an adjuvant therapy in enhancement of antioxidant capacity: A systematic review and meta-analysis of controlled clinical trials. Int J Clin Pract. 2021 Oct;75(10):e14618. doi: 10.1111/ijcp.14618. Epub 2021 Aug 4.

Reference Type RESULT
PMID: 34235823 (View on PubMed)

Moradi S, Ziaei R, Foshati S, Mohammadi H, Nachvak SM, Rouhani MH. Effects of Spirulina supplementation on obesity: A systematic review and meta-analysis of randomized clinical trials. Complement Ther Med. 2019 Dec;47:102211. doi: 10.1016/j.ctim.2019.102211. Epub 2019 Oct 17.

Reference Type RESULT
PMID: 31780031 (View on PubMed)

Hernandez-Lepe MA, Lopez-Diaz JA, Juarez-Oropeza MA, Hernandez-Torres RP, Wall-Medrano A, Ramos-Jimenez A. Effect of Arthrospira (Spirulina) maxima Supplementation and a Systematic Physical Exercise Program on the Body Composition and Cardiorespiratory Fitness of Overweight or Obese Subjects: A Double-Blind, Randomized, and Crossover Controlled Trial. Mar Drugs. 2018 Oct 1;16(10):364. doi: 10.3390/md16100364.

Reference Type RESULT
PMID: 30275428 (View on PubMed)

DiNicolantonio JJ, Bhat AG, OKeefe J. Effects of spirulina on weight loss and blood lipids: a review. Open Heart. 2020 Mar 8;7(1):e001003. doi: 10.1136/openhrt-2018-001003. eCollection 2020.

Reference Type RESULT
PMID: 32201580 (View on PubMed)

Related Links

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https://pubmed.ncbi.nlm.nih.gov/21686173/

Inflammation, oxidative stress, and obesity

https://pubmed.ncbi.nlm.nih.gov/30814686/

Obesity: global epidemiology and pathogenesis

https://pubmed.ncbi.nlm.nih.gov/30577684/

Inflammation and Oxidative Stress in an Obese State and the Protective Effects of Gallic Acid

https://pubmed.ncbi.nlm.nih.gov/34466462/

Comparison of Antioxidant and Anti-Inflammatory Effects of Honey and Spirulina platensis with Sulfasalazine and Mesalazine on Acetic Acid-Induced Ulcerative Colitis in Rats

https://doi.org/10.1016/j.jff.2022.105124

Anti-inflammatory and antithrombotic properties of polar lipid extracts, rich in unsaturated fatty acids, from the Irish marine cyanobacterium Spirulina subsalsa

https://cris.usil.edu.pe/en/publications/impact-of-spirulina-supplementation-on-obesity-related-metabolic-

Impact of spirulina supplementation on obesity-related metabolic disorders: A systematic review and meta-analysis of randomized controlled trials

Other Identifiers

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CT-390

Identifier Type: -

Identifier Source: org_study_id

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