Deciphering the Vitiligo Transcriptomic Signature Between Repigmented and Non Repigmented Lesions

NCT ID: NCT05927272

Last Updated: 2024-04-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

10 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-04-10

Study Completion Date

2025-10-09

Brief Summary

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The purpose of this study is to perform an in-depth analysis of the molecular pathways involved in lesions responding to current treatment and lesions not responding to current treatment

Detailed Description

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Treatment strategy: combination of oral mini-pulse of methylprednisolone 16mg twice a week + phototherapy Ultraviolet B (UVB) TL01, 2 times a week.

One skin biopsy will be performed at baseline and two skin biopsies at month 3 and month 6 on the same area forearms (excluding skin folds) Blood samples will be taken (30 mL) at week 0, week 12, week 24 for the measurement of pro-inflammatory cytokines and chemokines ligands in blood sera.

Principal Objective:

The aim of this study is to perform an in-depth analysis of vitiligo skin and blood samples taken during the course of a standard therapy to identify pathways involved in the repigmentation.

Secondary Objectives:

* Evolution of Vitiligo Disease in relation of patient profile
* Evolution of the activity of vitiligo in relation of patient profile The statistical and bioinformatics analyzes will be carried out on the data resulting from research with correlation to clinical data.

Conditions

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Vitiligo

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

monocentric, open-label study, and translational research.
Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Patients with active non-segmental vitiligo

Group Type EXPERIMENTAL

SKIN SAMPLES

Intervention Type PROCEDURE

At baseline: one 4 mm Ø skin biopsies: on forearms (excluding skin folds): fresh At month 3 and month 6: Two 4 mm Ø skin biopsies will be performed on the same lesion selected at baseline (one on a repigmented area, one on a non-responded area): fresh

Fresh Skin biopsies will be analyzed the same day using Single-cell RNA sequencing (scRNAseq).

BLOOD SAMPLES

Intervention Type PROCEDURE

Blood samples will be taken (30 mL) at week 0, week 12, week 24 for the measurement of pro-inflammatory cytokines and chemokines ligands in blood sera.

narrowband UVB

Intervention Type DEVICE

narrowband UVB (Nb-UVB) 2 times a week

Systemic Steroids

Intervention Type DRUG

Oral minipuls of systemic steroids (Medrol 16mg twice a week) for 24 weeks

Interventions

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SKIN SAMPLES

At baseline: one 4 mm Ø skin biopsies: on forearms (excluding skin folds): fresh At month 3 and month 6: Two 4 mm Ø skin biopsies will be performed on the same lesion selected at baseline (one on a repigmented area, one on a non-responded area): fresh

Fresh Skin biopsies will be analyzed the same day using Single-cell RNA sequencing (scRNAseq).

Intervention Type PROCEDURE

BLOOD SAMPLES

Blood samples will be taken (30 mL) at week 0, week 12, week 24 for the measurement of pro-inflammatory cytokines and chemokines ligands in blood sera.

Intervention Type PROCEDURE

narrowband UVB

narrowband UVB (Nb-UVB) 2 times a week

Intervention Type DEVICE

Systemic Steroids

Oral minipuls of systemic steroids (Medrol 16mg twice a week) for 24 weeks

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* Subject: male or female aged ≥ 18 years and ≤ 65 years
* Diagnosis of non-segmental (symmetrical) vitiligo with a body surface area involved \>5% excluding hands and feet, with lesions located on arms
* Active non-segmental vitiligo is defined by:

* Non-segmental vitiligo with new patches or extension of old lesions during the last 6 months AND
* Presence of hypochromic aspect under Wood's lamp examination and/or perifollicular hypopigmentation under Wood's lamp examination.
* Able to read, understand, and give documented informed consent
* Registered in the French Social Security
* Patients that could receive the combination of oral steroids and phototherapy according the recommendation
* Signed informed consent form

Exclusion Criteria

* Segmental or mixed vitiligo
* Are unable or unwilling to make themselves available for the duration of the study and/or are unwilling to follow study restrictions/procedures.
* Are currently enrolled in any other clinical trial involving an investigational product or any other type of medical research judged not to be scientifically or medically compatible with this study.
Minimum Eligible Age

18 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Centre National de la Recherche Scientifique, France

OTHER

Sponsor Role collaborator

University Hospital, Bordeaux

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Julien SENESCHAL, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

University Hospital, Bordeaux

Locations

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University Hospital of Bordeaux - St André Hospital

Bordeaux, , France

Site Status RECRUITING

Countries

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France

Central Contacts

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Julien SENESCHAL, MD, PhD

Role: CONTACT

+33 (0)5 56 79 49 63

Sarah CAZENAVE

Role: CONTACT

+335 57 82 26 63

Facility Contacts

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Julien SENESCHAL, MD, PhD

Role: primary

+335.56.79.47.05

Nicolas ANDREU

Role: backup

+335 57 82 06 55

Other Identifiers

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CHUBX 2021/54

Identifier Type: -

Identifier Source: org_study_id

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