Transplantation of Basal Cell Layer Suspension Using Derma-rolling System in Vitiligo

NCT ID: NCT02962180

Last Updated: 2016-11-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

10 participants

Study Classification

INTERVENTIONAL

Study Start Date

2016-03-31

Study Completion Date

2017-12-31

Brief Summary

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Actually the methods for melanocyte delivery are invasive and often sophisticated. The dermarolling system with needles causing tiny microinjuries in the epidermis could offer a minimally invasive and painless method of melanocyte transplantation. The purpose of the study is to develop a new and simple method for transepidermally delivering keratinocytes and melanocytes into vitiligo skin.

Detailed Description

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Condition: stable lesion of non segmental and segmental vitiligo of adults.

Background: The best method to transepidermally deliver isolated cells (melanocytes) is not yet defined. Microneedle treatment of the " ex vivo " human skin caused many thin vertical epidermodermal fissures and a variable depth of injury into the dermis regarding the length of the needles. This kind of treatment could be able to create small epidermal defects which allows to deliver melanocytes to an epidermal site. An approach to replenish melanocytes by injection of dissociated epidermal cell suspension with a syringe was recently successfully used. So, we hypothesize that the use of dermaroller with 0.2mm needle length which causes tiny microinjuries strictly in the epidermis could offer a simple, minimally invasive and painless method of melanocyte transplantation.

Main objective: To demonstrate the efficacy and the interest of dermaroller use with 0.2mm needle length in the transplantation of basal cell layer suspension in the epidermis of depigmented vitiligo skin.

Secondary objectives

1. To assess as control the effect of the use of dermaroller alone on the vitiligo lesion.
2. If transplantation is successful, to assess the duration needed to obtain a good coalescence and a complete repigmentation of the vitiligo lesion.

Conditions

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Vitiligo

Keywords

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derma-rolling system, vitiligo

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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single arm study

Dermabrasion with dermaroller of basal cell layer suspension obtained by soft trypsinisation in vitiligo lesion.

Group Type EXPERIMENTAL

Dermabrasion with dermaroller

Intervention Type DEVICE

o The dermaroller is passed on the treated depigmented area. This technique is based on tiny microwounds performed using 540 microneedles (0.2mm length) which should allow the penetration of the suspension cells into the epidermis to obtain a good repigmentation.

Interventions

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Dermabrasion with dermaroller

o The dermaroller is passed on the treated depigmented area. This technique is based on tiny microwounds performed using 540 microneedles (0.2mm length) which should allow the penetration of the suspension cells into the epidermis to obtain a good repigmentation.

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* Non segmental and segmental vitiligo lesions stable for a minimum of 1 year prior surgery
* Men and women aged over 18 years of age
* The lesion area must be 2cm2\< lesion\< 15cm2
* Absence of infected lesion

Exclusion Criteria

* Actively spreading vitiligo (unstable disease)
* Patient with history of melanoma
* Infected lesions
* Positive Koebner Phenomenon
* History of hypertrophic scars or keloid formation
* Treatment with immunosuppressive or cytotoxic medication the past year
* Pregnant women and patients aged less 18 years
* Patients with concomitant photosensitizing treatment
* Positive serology of herpes, HIV, hepatitis B and C
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Mohammed V Souissi University

OTHER

Sponsor Role lead

Responsible Party

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BENZEKRI LAILA

PhD

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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laila benzekri

Role: PRINCIPAL_INVESTIGATOR

Mohammed V University

Locations

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Department of Dermatology, Ibn Sina University Hospital

Rabat, Morocco, Morocco

Site Status RECRUITING

Countries

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Morocco

Central Contacts

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laila benzekri, PhD

Role: CONTACT

Phone: 212671964111

Email: [email protected]

Facility Contacts

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LAILA BENZEKRI, PhD

Role: primary

References

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Khodadadi L, Shafieyan S, Sotoudeh M, Dizaj AV, Shahverdi A, Aghdami N, Baharvand H. Intraepidermal injection of dissociated epidermal cell suspension improves vitiligo. Arch Dermatol Res. 2010 Oct;302(8):593-9. doi: 10.1007/s00403-010-1034-7. Epub 2010 Apr 4.

Reference Type RESULT
PMID: 20364383 (View on PubMed)

Other Identifiers

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MOHAMMED V UNIVERSITY

Identifier Type: -

Identifier Source: org_study_id