Production of Gut Microbiota-Derived Metabolites in Response to Different Fatty Acid Profiles

NCT ID: NCT05917132

Last Updated: 2025-06-27

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 49 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2023-06-12
• Study Completion Date: 2024-12-01

Brief Summary

The proposed project mainly aims to investigate the microbial processes leading to dietary metabolites production, independently of long-term microbiota adaptation to the diet, by measuring the microbiota-derived metabolite production from a meal sequence rich in saturated fatty acids (SFA) or the same meals but rich in polyunsaturated fatty acids (PUFA) in individuals with or without obesity and Type 2 diabetes (T2D)

Detailed Description

The gut microbiota interacts with the host's enteric and systemic functions through the production of gut microbiota-derived metabolites. A subset of these metabolites, the endocannabinoids (eCB) and their congeners are derived from fatty acids and play a crucial role in metabolic health.

The research project aims to study gut microbiota-derived metabolites, including the eCB and their congeners, in response to a 2-day diet rich in polyunsaturated fatty acids or rich in saturated fatty acids in individuals with or without abdominal obesity and type 2 diabetes.

The investigators hope to better understand the kinetic production of circulating endocannabinoidome mediators derived from fatty acids in the diet.

Conditions

Obesity, Abdominal; Diabete Type 2

Study Design

• Allocation Method: NA
• Intervention Model: SEQUENTIAL
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

Single

All participating subjects will undergo 3 feeding phases : 3 days of a run-in diet, 2 days of a polyunsaturated fatty acid (PUFA) diet and 2 days of a saturated fatty acid (SFA) diet.

Group Type: EXPERIMENTAL

Nutritionnal intervention

Intervention Type: OTHER

Participants will be provided with meals corresponding to each diet during the week. Participants will be required to eat only the meals provided.

Interventions

Nutritionnal intervention

Participants will be provided with meals corresponding to each diet during the week. Participants will be required to eat only the meals provided.

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Men and women aged between 45 and 65 years, menopaused women ;
• Body mass index between 20 to 35 kg/m² ;
• General good health ;
• Score of diet quality (HEI) between 40 and 70 ;
• Computer and Internet access ;
• Basic understanding of written French ;
• Daily stool frequency.

Exclusion Criteria

• Intestinal pathologies including inflammatory bowel disease and gastrointestinal cancers ;
• Alcohol consumption greater than 15 portions for men and 10 for women weekly.
• Active tobacco and cannabis usage ;
• Consumption of omega-3 dietary supplements ;
• Consumption of dietary supplements (vitamins and probiotics) - must be ceased 2 weeks before the dietary intervention ;
• Completion of a course of antibiotics in the past 3 months ;
• Important weight change (+/- 5 kg) in the past 6 months ;
• Type 1 diabetes or insulin-treated diabetes.

• Minimum Eligible Age: 45 Years
• Maximum Eligible Age: 65 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Laval University

OTHER

Sponsor Role: lead

Responsible Party

Alain Veilleux

Professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Alain Veilleux, PhD

Role: PRINCIPAL_INVESTIGATOR

Laval University

Locations

Institut sur la nutrition et les aliments fonctionnels - INAF

Québec, Quebec, Canada

Countries

Canada

Other Identifiers

2022-109

Identifier Type: -

Identifier Source: org_study_id