Replacing Invasive Cystoscopy With Urine Testing for Non-muscle Invasive Bladder Cancer Surveillance

NCT ID: NCT05796375

Last Updated: 2025-11-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

240 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-11-17

Study Completion Date

2028-12-30

Brief Summary

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The purpose of this research is to determine whether bladder cancer monitoring can be improved by replacing some cystoscopy procedures with urine testing. Specifically, this study examines whether there are any differences in urinary symptoms, discomfort, number of invasive procedures, anxiety, complications, cancer recurrence or cancer progression when some cystoscopy procedures are replaced with urine testing.

Detailed Description

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This is a multi-site randomized phase 2 trial including 240 patients with early-stage bladder cancer, in which patients will be randomized 1:1:1 to programmatic surveillance with the Xpert bladder cancer urine test, the Bladder EpiCheck urine test, or frequent cystoscopy. The primary outcome will be urinary quality of life measured 1 to 3 days after surveillance.

This study will have three groups, also called "arms": (1) Frequent Cystoscopy Arm, (2) Xpert Urine Test Arm, and (3) Epicheck Urine Test Arm. The aim of Frequent Cystoscopy is to detect any cancer that might have come back within the bladder by frequently inspecting the bladder. Those in the cystoscopy arm will have a cystoscopy procedure at specified time points for two years. The goal of the Xpert Urine Test arm is to detect any cancer that might have come back within the bladder, while decreasing the number of invasive cystoscopy procedures. Those in the Xpert arm will have a Xpert urine test and a check-up with a medical doctor at 6 months and 18 months, and have a cystoscopy procedure at 12 months and 24 months. The aim of the EpiCheck Urine Test arm is to detect any cancer that might have come back within the bladder, while decreasing the number of invasive cystoscopy procedures. Those in the EpiCheck Urine Test arm will have an Epicheck urine test and a check-up with a medical doctor at 6 months and 18 months and will have a cystoscopy procedure at 12 months and 24 months. The study performance period is 24 months.

Conditions

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Non-muscle-invasive Bladder Cancer

Keywords

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Bladder Cancer Urology Cystoscopy Xpert EpiCheck Urine test

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Health care delivery parallel trial with 1:1:1 randomization
Primary Study Purpose

HEALTH_SERVICES_RESEARCH

Blinding Strategy

NONE

Study Groups

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Frequent Cystoscopy

Cystoscopy is conducted at 6, 12, 18, and 24 months for patients undergoing surveillance for low grade intermediate-risk non-muscle invasive bladder cancer

Group Type ACTIVE_COMPARATOR

Cystoscopy

Intervention Type PROCEDURE

Cystoscopy entails direct inspection of the bladder via a cystoscope that is inserted into a patient's urethra.

Xpert Urine Test

Xpert arm includes urine testing using the Xpert Bladder Cancer Monitor urine test at 6 and 18 months. Cystoscopy is conducted at 12 and 24 months for patients undergoing surveillance for low grade intermediate-risk non-muscle invasive bladder cancer

Group Type EXPERIMENTAL

Cystoscopy

Intervention Type PROCEDURE

Cystoscopy entails direct inspection of the bladder via a cystoscope that is inserted into a patient's urethra.

Xpert Bladder Cancer Monitor urine test

Intervention Type DIAGNOSTIC_TEST

A 4.5 ml sample of voided urine is added to Xpert® Urine Transport Reagent, mixed, and then 4ml of treated urine are transferred to the Sample Chamber of the cartridge. In the cartridge, cells in the urine sample are captured on a filter and lysed by sonication. The released nucleic acid is eluted, mixed with dry RT-PCR reagents, and the solution is transferred to the reaction tube for RT-PCR and detection.

EpiCheck Urine Text

EpiCheck arm includes urine testing using the Bladder EpiCheck urine test at 6 and 18 months. Cystoscopy is conducted at 12 and 24 months for patients undergoing surveillance for low grade intermediate-risk non-muscle invasive bladder cancer.

Group Type EXPERIMENTAL

Cystoscopy

Intervention Type PROCEDURE

Cystoscopy entails direct inspection of the bladder via a cystoscope that is inserted into a patient's urethra.

Bladder EpiCheck urine test

Intervention Type DIAGNOSTIC_TEST

The Bladder EpiCheck is a laboratory-developed test for early-stage non-muscle invasive bladder cancer. The Bladder EpiCheck test is a DNA methylation test that is run on DNA extracted from cell pellet from centrifuged urine according to Standardized Operating Procedures. The test has internal controls and a dedicated software to ensure required quality assurance processes and prompt reporting of results.

Interventions

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Cystoscopy

Cystoscopy entails direct inspection of the bladder via a cystoscope that is inserted into a patient's urethra.

Intervention Type PROCEDURE

Bladder EpiCheck urine test

The Bladder EpiCheck is a laboratory-developed test for early-stage non-muscle invasive bladder cancer. The Bladder EpiCheck test is a DNA methylation test that is run on DNA extracted from cell pellet from centrifuged urine according to Standardized Operating Procedures. The test has internal controls and a dedicated software to ensure required quality assurance processes and prompt reporting of results.

Intervention Type DIAGNOSTIC_TEST

Xpert Bladder Cancer Monitor urine test

A 4.5 ml sample of voided urine is added to Xpert® Urine Transport Reagent, mixed, and then 4ml of treated urine are transferred to the Sample Chamber of the cartridge. In the cartridge, cells in the urine sample are captured on a filter and lysed by sonication. The released nucleic acid is eluted, mixed with dry RT-PCR reagents, and the solution is transferred to the reaction tube for RT-PCR and detection.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

1. Aged 18 years or older
2. History of low grade intermediate-risk non-muscle invasive bladder cancer, defined as most recent pathology report showing any of the following:

* multifocal low grade non-invasive urothelial carcinoma of any size
* solitary low grade non-invasive urothelial carcinoma greater than 3cm in size
* recurrent low grade non-invasive urothelial carcinoma
3. Stated willingness to comply with all study procedures and availability for the duration of the study
4. No evidence for recurrence at cystoscopy ≤6 months after most recent tumor resection
5. Ability to consent in English or Spanish

Exclusion Criteria

1. History of total cystectomy of the bladder.
2. History of urinary diversion (e.g., neo-bladder, colon pouch, or ileal conduit).
3. History of muscle-invasive bladder tumor.
4. Pregnancy or lactation.
5. History of urothelial carcinoma of the ureter or renal pelvis status post endoscopic treatment or with evidence of recurrent upper tract disease (inclusion allowed if status post nephroureterectomy and recurrence free at time of inclusion)
6. Anatomic constraints making cystoscopy impossible (e.g., history of urethrectomy, obliterated urethra secondary to stricture).
7. Inability to provide a voided urine sample.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

University of Texas Southwestern Medical Center

OTHER

Sponsor Role collaborator

Medical University of South Carolina

OTHER

Sponsor Role collaborator

White River Junction Veterans Affairs Medical Center

FED

Sponsor Role lead

Responsible Party

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Florian Schroeck

Chief, Section of Urology

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Florian R Schroeck, MD, MS

Role: PRINCIPAL_INVESTIGATOR

White River Junction VA Healthcare System

Locations

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West Haven VA Medical Center

West Haven, Connecticut, United States

Site Status RECRUITING

Bay Pines VA Healthcare System

Bay Pines, Florida, United States

Site Status RECRUITING

VA Boston Jamaica Plains Campus

West Roxbury, Massachusetts, United States

Site Status RECRUITING

VA St.Louis Healthcare System

St Louis, Missouri, United States

Site Status RECRUITING

University Hospitals Cleveland Medical Center

Cleveland, Ohio, United States

Site Status RECRUITING

Medical University of South Carolina

Charleston, South Carolina, United States

Site Status ACTIVE_NOT_RECRUITING

UT Southwestern Medical Center

Dallas, Texas, United States

Site Status RECRUITING

White River Junction Veterans Healthcare System

White River Junction, Vermont, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Florian R Schroeck, MD, MS

Role: CONTACT

Phone: 802-295-9363

Email: [email protected]

Laura Jensen, MPH

Role: CONTACT

Phone: 802-280-5298

Email: [email protected]

Facility Contacts

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Alicia Roy

Role: primary

Anna Rosenblatt

Role: primary

Laura MacAdam, NP

Role: primary

Carley Browning

Role: primary

Imani Lucas, MPH

Role: primary

Sonobia Garrett, BSHS

Role: primary

Laura L Jensen, MPH

Role: primary

References

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Koo K, Zubkoff L, Sirovich BE, Goodney PP, Robertson DJ, Seigne JD, Schroeck FR. The Burden of Cystoscopic Bladder Cancer Surveillance: Anxiety, Discomfort, and Patient Preferences for Decision Making. Urology. 2017 Oct;108:122-128. doi: 10.1016/j.urology.2017.07.016. Epub 2017 Jul 21.

Reference Type BACKGROUND
PMID: 28739405 (View on PubMed)

Schroeck FR, Lynch KE, Li Z, MacKenzie TA, Han DS, Seigne JD, Robertson DJ, Sirovich B, Goodney PP. The impact of frequent cystoscopy on surgical care and cancer outcomes among patients with low-risk, non-muscle-invasive bladder cancer. Cancer. 2019 Sep 15;125(18):3147-3154. doi: 10.1002/cncr.32185. Epub 2019 May 23.

Reference Type BACKGROUND
PMID: 31120559 (View on PubMed)

Schroeck FR, Grubb R, MacKenzie TA, Ould Ismail AA, Jensen L, Tsongalis GJ, Lotan Y. Clinical Trial Protocol for "Replace Cysto": Replacing Invasive Cystoscopy with Urine Testing for Non-muscle-invasive Bladder Cancer Surveillance-A Multicenter, Randomized, Phase 2 Healthcare Delivery Trial Comparing Quality of Life During Cancer Surveillance with Xpert Bladder Cancer Monitor or Bladder EpiCheck Urine Testing Versus Frequent Cystoscopy. Eur Urol Open Sci. 2024 Mar 21;63:19-30. doi: 10.1016/j.euros.2024.02.018. eCollection 2024 May.

Reference Type BACKGROUND
PMID: 38558761 (View on PubMed)

Other Identifiers

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R37CA275916

Identifier Type: NIH

Identifier Source: secondary_id

View Link

1R37CA275916-01

Identifier Type: NIH

Identifier Source: secondary_id

View Link

1732756

Identifier Type: -

Identifier Source: org_study_id