TSC Biosample Repository and Natural History Database

NCT ID: NCT05676099

Last Updated: 2025-07-08

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

5000 participants

Study Classification

OBSERVATIONAL

Study Start Date

2016-01-31

Study Completion Date

2050-12-31

Brief Summary

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The TSC Biosample Repository collects and stores samples of blood, DNA, and tissues that scientists can request to use in their research. The samples we collect are all linked to clinical data in the TSC Natural History Database. The TSC Natural History Database captures clinical data to document the impact of the disease on a person's health over his or her lifetime. This data may be collected retrospectively or prospectively.

Detailed Description

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The purpose of the project which is sponsored by the TSC Alliance is to learn more about tuberous sclerosis complex (TSC) which may lead to new treatments for conditions that affect different areas of the body such as the brain, kidney, heart, lungs, and skin. The TSC Alliance TSC Biosample Repository (BSR) was established to provide a central biobank at the Van Andel Institute (VAI) Biorepository in Grand Rapids, Michigan for the collection of blood, tissues, and cells from a vast number of individuals with TSC.

The TSC Alliance Natural History Database (NHD), established in 2006, will serve as the central repository of de-identified clinical data associated with biosamples collected from individuals with TSC. The NHD research project involves collection of retrospective and prospective private information on individuals with a diagnosis of TSC over their lifespan (i.e., a longitudinal study). The VAI Biorepository will distribute biosamples and NHD data to researchers as approved by the TSC Alliance.

This project also aims to collect biosamples and clinical data on people affected by sporadic lymphangioleiomyomatosis (sporadic LAM). LAM is a common symptom reported in TSC that may occur outside the context of a TSC diagnosis (i.e., sporadic LAM patients).

The collection of biosamples will be at a clinical study site (CSS) such as a TSC Alliance recognized TSC clinic, a non-CSS such as a participant's home, an educational meeting, or by other clinical partners (CP) with institutional review board (IRB) approval of this protocol and informed consent forms. Collection of biosamples may also occur at a non-CSS or by a licensed phlebotomist (e.g., via partnership with mobile phlebotomy companies). The VAI Biorepository will provide collection kits, instructions, and materials to the CSS, non-CSS, CP, or directly to participant.

The CSS, CP, non-CSS, or authorized representative will ship collected biosamples to the VAI Biorepository for processing and storage according to their IRB-approved standard operating procedures. The VAI Biorepository will distribute biosamples to investigators as approved by the TSC Alliance. Their accreditation under the Biorepository Accreditation Program of the College of American Pathologists (CAP) will stand as the governing rules for best practices. Distribution of biosamples will require receipt of the investigator's IRB approval and a material transfer agreement (MTA) executed between the approved investigator and the TSC Alliance.

Clinical data in the NHD associated with a biosample will be provided to an investigator as approved by the Natural History Database-Biosample Repository (NHD-BSR) Steering Committee.

This project is open to individuals of all ages with a diagnosis of tuberous sclerosis complex or lymphangioleiomyomatosis.

Conditions

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Tuberous Sclerosis Lymphangioleiomyomatosis

Study Design

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Observational Model Type

OTHER

Study Time Perspective

OTHER

Interventions

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Phlebotomy

Participants may elect to submit a blood sample to the Biosample Repository.

Intervention Type PROCEDURE

Buccal (cheek) swab

Participants may elect to submit a buccal swab sample to the Biosample Repository.

Intervention Type PROCEDURE

Genetic Testing

Biosamples may be processed and analyzed for genetic variants using whole genome sequencing (WGS) or other sequencing methods. Participants whose samples are processed in this manner may be contacted and provided the option to receive TSC1 or TSC2 genetic variant results by opting in using Consent to Return of Genetic Results Form. Participants will be offered a one-time genetic counseling session to review their results, free of charge. CLIA-certified, TSC1 or TSC2 genetic variant results will be returned to participants who opt in to receive such results. Additionally, negative results and results not able to be clinically certified will also be offered to participants with a one-time genetic counseling session to review their results, free of charge using the Return of Genetic Research Results Template Letter. CSS will be responsible for informing clinic participants that their samples have been sequenced and offer to connect participant to the TSC Alliance for further information.

Intervention Type GENETIC

Tissue donation after routine clinical procedure

Participants may elect to submit a tissue sample to the Biosample Repository following a medical procedure.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Diagnosis of tuberous sclerosis complex or lymphangioleiomyomatosis (sporadic LAM).

Exclusion Criteria

\-
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Tuberous Sclerosis Association

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Steve Roberds, PhD

Role: PRINCIPAL_INVESTIGATOR

TSC Alliance

Locations

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University of Alabama Birmingham

Birmingham, Alabama, United States

Site Status RECRUITING

Loma Linda University Children's Hospital

Loma Linda, California, United States

Site Status ACTIVE_NOT_RECRUITING

University of California Los Angeles

Los Angeles, California, United States

Site Status RECRUITING

Jack & Julia Center for TSC, Oakland Children's Hospital and Research Center

Oakland, California, United States

Site Status SUSPENDED

The Children's Hospital

Denver, Colorado, United States

Site Status ACTIVE_NOT_RECRUITING

Nicklaus Children's Hospital

Miami, Florida, United States

Site Status RECRUITING

Chicago Comer Children's Hospital Neurogenetic Clinic, University of Chicago

Chicago, Illinois, United States

Site Status ACTIVE_NOT_RECRUITING

University of Iowa Hospitals and Clinics

Iowa City, Iowa, United States

Site Status RECRUITING

TSC Alliance

Silver Spring, Maryland, United States

Site Status RECRUITING

Boston Children's Hospital

Boston, Massachusetts, United States

Site Status RECRUITING

Massachusetts General Hospital

Boston, Massachusetts, United States

Site Status RECRUITING

Minnesota Epilepsy Group

Roseville, Minnesota, United States

Site Status RECRUITING

Washington University in St. Louis

St Louis, Missouri, United States

Site Status RECRUITING

New York University Medical Center

New York, New York, United States

Site Status RECRUITING

Cincinnati Children's Hospital Medical Center

Cincinnati, Ohio, United States

Site Status RECRUITING

Cleveland Clinic Foundation

Cleveland, Ohio, United States

Site Status RECRUITING

University of Pennsylvania Medical Center

Philadelphia, Pennsylvania, United States

Site Status ACTIVE_NOT_RECRUITING

Le Bonheur Children's Hospital

Memphis, Tennessee, United States

Site Status RECRUITING

Scottish Rite Hospital for Children

Dallas, Texas, United States

Site Status RECRUITING

Texas Childrens Hospital Baylor College of Medicine

Houston, Texas, United States

Site Status RECRUITING

Memorial Hermann-Texas Medical Center (University of Texas Houston)

Houston, Texas, United States

Site Status RECRUITING

Children's National Medical Center

Fairfax, Virginia, United States

Site Status RECRUITING

Centre Hospitalier de L'Université de Montréal (Chum)

Montreal, , Canada

Site Status RECRUITING

Sainte-Justine Université de Montréal

Montreal, , Canada

Site Status RECRUITING

Countries

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United States Canada

Central Contacts

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Elizabeth Cassidy, MPH

Role: CONTACT

301-562-9890

Ayat Abi

Role: CONTACT

Facility Contacts

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Jessica Krefting, RN, BSN

Role: primary

(205) 975-2890

Martina Bebin, M.D., M.P.A.

Role: backup

(256)-533-0833

Ruby Escalante

Role: primary

310-206-5586

Angela Martinez

Role: backup

(310) 206-7630

Debbie Newman

Role: primary

786-624-2853

Paola Garcia

Role: backup

Liberty Taeger

Role: primary

(319)356-8427

Michael Ciliberto, MD

Role: backup

Elizabeth Cassidy, MPH

Role: primary

301-562-9890

Ayat Abi

Role: backup

Anna Cronin, MPH

Role: primary

617-919-3499

Carolyn Wilson

Role: primary

Nick Manickas-Hill

Role: backup

Sarah Ellis, CCRC

Role: primary

651-377-8319

Shadow Lor

Role: backup

Ashley Fasciola, RN, BSN

Role: primary

314-454-6120

Michael Wong, MD, PhD

Role: backup

RC Fletcher

Role: primary

Mariana Figuera Losada

Role: backup

Salma Ihiri

Role: primary

513-636-8016

Molly Griffith

Role: backup

Honglian Huang

Role: primary

Sherry Fu

Role: backup

Ashley Peterson, BSN, RN

Role: primary

901-287-5168

Catherine Thompson, CCRP

Role: primary

214-559-7818

Sarah Wisor-Martinez

Role: primary

Claire Sartwell, RN, BSN

Role: primary

Mariam Lugo Noguera

Role: backup

713-500-6350

Nancy Elling, RN, BSN, CPN

Role: primary

703-635-2873

William McClintock, MD

Role: backup

Mark Keezer, MSc, MDCM, PhD

Role: primary

Ya Ning Zhao, MSc

Role: backup

Maryse Thibeault

Role: primary

References

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Pounders AJ, Rushing GV, Mahida S, Nonyane BAS, Thomas EA, Tameez RS, Gipson TT. Racial differences in the dermatological manifestations of tuberous sclerosis complex and the potential effects on diagnosis and care. Ther Adv Rare Dis. 2022 Dec 10;3:26330040221140125. doi: 10.1177/26330040221140125. eCollection 2022 Jan-Dec.

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Rubtsova VI, Chun Y, Kim J, Ramirez CB, Jung S, Choi W, Kelly ME, Lopez ML, Cassidy E, Rushing G, Aguiar DJ, Lau WL, Ahdoot RS, Smith M, Edinger AL, Lee SG, Jang C, Lee G. Circulating biomarkers of kidney angiomyolipoma and cysts in tuberous sclerosis complex patients. iScience. 2024 Jun 13;27(7):110265. doi: 10.1016/j.isci.2024.110265. eCollection 2024 Jul 19.

Reference Type BACKGROUND
PMID: 39027368 (View on PubMed)

Parthasarathy S, Mahalingam R, Melchiorre J, Harowitz J, Devinsky O. Mortality in tuberous sclerosis complex. Epilepsy Behav. 2021 Aug;121(Pt A):108032. doi: 10.1016/j.yebeh.2021.108032. Epub 2021 Jun 1.

Reference Type BACKGROUND
PMID: 34087679 (View on PubMed)

Chivukula S, Modiri O, Kashanian A, Babayan D, Ibrahim GM, Weil AG, Tu A, Wu JY, Mathern GW, Fallah A. Effect of Gene Mutation on Seizures in Surgery for Tuberous Sclerosis Complex. Can J Neurol Sci. 2021 May;48(3):327-334. doi: 10.1017/cjn.2020.185. Epub 2020 Aug 28.

Reference Type BACKGROUND
PMID: 32854808 (View on PubMed)

Gupta A, de Bruyn G, Tousseyn S, Krishnan B, Lagae L, Agarwal N; TSC Natural History Database Consortium. Epilepsy and Neurodevelopmental Comorbidities in Tuberous Sclerosis Complex: A Natural History Study. Pediatr Neurol. 2020 May;106:10-16. doi: 10.1016/j.pediatrneurol.2019.12.016. Epub 2020 Feb 4.

Reference Type BACKGROUND
PMID: 32139167 (View on PubMed)

Song J, Swallow E, Said Q, Peeples M, Meiselbach M, Signorovitch J, Kohrman M, Korf B, Krueger D, Wong M, Sparagana S. Epilepsy treatment patterns among patients with tuberous sclerosis complex. J Neurol Sci. 2018 Aug 15;391:104-108. doi: 10.1016/j.jns.2018.06.011. Epub 2018 Jun 15.

Reference Type BACKGROUND
PMID: 30103955 (View on PubMed)

Jeong A, Nakagawa JA, Wong M. Predictors of Drug-Resistant Epilepsy in Tuberous Sclerosis Complex. J Child Neurol. 2017 Dec;32(14):1092-1098. doi: 10.1177/0883073817737446.

Reference Type BACKGROUND
PMID: 29129154 (View on PubMed)

Jeong A, Wong M. Systemic disease manifestations associated with epilepsy in tuberous sclerosis complex. Epilepsia. 2016 Sep;57(9):1443-9. doi: 10.1111/epi.13467. Epub 2016 Jul 15.

Reference Type BACKGROUND
PMID: 27417921 (View on PubMed)

Kothare SV, Singh K, Hochman T, Chalifoux JR, Staley BA, Weiner HL, Menzer K, Devinsky O. Genotype/phenotype in tuberous sclerosis complex: associations with clinical and radiologic manifestations. Epilepsia. 2014 Jul;55(7):1020-4. doi: 10.1111/epi.12627. Epub 2014 Apr 22.

Reference Type BACKGROUND
PMID: 24754401 (View on PubMed)

Kothare SV, Singh K, Chalifoux JR, Staley BA, Weiner HL, Menzer K, Devinsky O. Severity of manifestations in tuberous sclerosis complex in relation to genotype. Epilepsia. 2014 Jul;55(7):1025-9. doi: 10.1111/epi.12680. Epub 2014 Jun 10.

Reference Type BACKGROUND
PMID: 24917535 (View on PubMed)

van Eeghen AM, Nellist M, van Eeghen EE, Thiele EA. Central TSC2 missense mutations are associated with a reduced risk of infantile spasms. Epilepsy Res. 2013 Jan;103(1):83-7. doi: 10.1016/j.eplepsyres.2012.07.007. Epub 2012 Aug 3.

Reference Type BACKGROUND
PMID: 22867869 (View on PubMed)

Ehninger D, Sano Y, de Vries PJ, Dies K, Franz D, Geschwind DH, Kaur M, Lee YS, Li W, Lowe JK, Nakagawa JA, Sahin M, Smith K, Whittemore V, Silva AJ. Gestational immune activation and Tsc2 haploinsufficiency cooperate to disrupt fetal survival and may perturb social behavior in adult mice. Mol Psychiatry. 2012 Jan;17(1):62-70. doi: 10.1038/mp.2010.115. Epub 2010 Nov 16.

Reference Type BACKGROUND
PMID: 21079609 (View on PubMed)

Boggarapu S, Roberds SL, Nakagawa J, Beresford E. Characterization and management of facial angiofibroma related to tuberous sclerosis complex in the United States: retrospective analysis of the natural history database. Orphanet J Rare Dis. 2022 Sep 14;17(1):355. doi: 10.1186/s13023-022-02496-2.

Reference Type BACKGROUND
PMID: 36104799 (View on PubMed)

Aronow ME, Nakagawa JA, Gupta A, Traboulsi EI, Singh AD. Tuberous sclerosis complex: genotype/phenotype correlation of retinal findings. Ophthalmology. 2012 Sep;119(9):1917-23. doi: 10.1016/j.ophtha.2012.03.020. Epub 2012 May 16.

Reference Type BACKGROUND
PMID: 22608477 (View on PubMed)

Mowrey K, Northrup H, Rougeau P, Hashmi SS, Krueger DA, Ebrahimi-Fakhari D, Towbin AJ, Trout AT, Capal JK, Franz DN, Rodriguez-Buritica D. Frequency, Progression, and Current Management: Report of 16 New Cases of Nonfunctional Pancreatic Neuroendocrine Tumors in Tuberous Sclerosis Complex and Comparison With Previous Reports. Front Neurol. 2021 Apr 9;12:627672. doi: 10.3389/fneur.2021.627672. eCollection 2021.

Reference Type BACKGROUND
PMID: 33897589 (View on PubMed)

Swallow E, King S, Song J, Peeples M, Signorovitch JE, Liu Z, Prestifilippo J, Frost M, Kohrman M, Korf B, Krueger D, Sparagana S. Patterns of Disease Monitoring and Treatment Among Patients With Tuberous Sclerosis Complex-related Angiomyolipomas. Urology. 2017 Jun;104:110-114. doi: 10.1016/j.urology.2017.02.036. Epub 2017 Mar 2.

Reference Type BACKGROUND
PMID: 28263820 (View on PubMed)

Hsieh LS, Wen JH, Nguyen LH, Zhang L, Getz SA, Torres-Reveron J, Wang Y, Spencer DD, Bordey A. Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice. Sci Transl Med. 2020 Nov 18;12(570):eabc1492. doi: 10.1126/scitranslmed.abc1492.

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PMID: 33208499 (View on PubMed)

Giannikou K, Martin KR, Abdel-Azim AG, Pamir KJ, Hougard TR, Bagwe S, Tang Y, MacKeigan JP, Kwiatkowski DJ, Henske EP, Lam HC. Spectrum of germline and somatic mitochondrial DNA variants in Tuberous Sclerosis Complex. Front Genet. 2023 Jan 30;13:917993. doi: 10.3389/fgene.2022.917993. eCollection 2022.

Reference Type BACKGROUND
PMID: 36793390 (View on PubMed)

Bhaoighill MN, Falcon-Perez JM, Royo F, Tee AR, Webber JP, Dunlop EA. Tuberous Sclerosis Complex cell-derived EVs have an altered protein cargo capable of regulating their microenvironment and have potential as disease biomarkers. J Extracell Vesicles. 2023 Jun;12(6):e12336. doi: 10.1002/jev2.12336.

Reference Type BACKGROUND
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Loubert F, House AA, Larochelle C, Major P, Keezer MR. Development and internal validation of a clinical risk score to predict incident renal and pulmonary tumours in people with tuberous sclerosis complex. J Med Genet. 2024 Sep 24;61(10):943-949. doi: 10.1136/jmg-2023-109717.

Reference Type BACKGROUND
PMID: 38977299 (View on PubMed)

Related Links

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Other Identifiers

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15039

Identifier Type: -

Identifier Source: org_study_id

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