GCF Levels of IL-1β and IL-6 in Gingivitis and Stage III-Grade C Periodontitis

NCT ID: NCT04689438

Last Updated: 2022-11-04

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 64 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2020-10-08
• Study Completion Date: 2020-12-28

Brief Summary

Periodontal diseases are chronic diseases that occur as a result of a violation of the balance between microbial dental plaque and the host response. Gingivitis is a disease characterized by inflammation of the gingiva that occurs in one or more areas without loss of attachments.1 in periodontitis, an inflammatory event that begins in the gingiva along with gingivitis spreads to the periodontal ligament, alveolar bone and soft tissues that support the tooth, causing the destruction of these structures.2 Cytokines are low molecular weight proteins that participate in the initial and active stages of inflammation and immunity. In periodontal disease pathogenesis, cytokine response has been reported to play a very critical role in determining disease progression.3 IL-1beta and IL-6 are key cytokines in chronic inflammatory diseases and have the potential to initiate bone loss and tissue destruction seen in periodontal disease.4the purpose of this study; it is to determine the degree of inflammation and periodontal destruction by determining the levels of IL-1beta and IL-6 cytokines in the gingival crevicular fluid of periodontal healthy and diseased individuals.

Detailed Description

Periodontal measurement were done at 6 sites per tooth After clinical evaluation, individuals were divided into 3 groups

1. Healthy control groups:

clinically healthy gingiva

2. Gingivitis groups:

BOP score of 10% or greater and PD≤3mm

3. Periodontitis groups:

AL ≥5 mm, PD≥6 mm and tooth loss due to periodontitis of ≤4. GCF samples were collected using strips.

Conditions

Periodontitis

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: PROSPECTIVE

Study Groups

Healthy control groups

clinically healthy gingiva BOP score less than 10%

gingival crevicular fluid

Intervention Type: DIAGNOSTIC_TEST

GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.

Gingivitis groups

BOP score of 10% or greater

gingival crevicular fluid

Intervention Type: DIAGNOSTIC_TEST

GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.

Periodontitis groups

interdental AL ≥5 mm, PD≥6 mm

gingival crevicular fluid

Intervention Type: DIAGNOSTIC_TEST

GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.

Interventions

gingival crevicular fluid

GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

• have periodontitis
• have 16 permanent teeth

Exclusion Criteria

• Systemic disease
• using drugs

• Minimum Eligible Age: 20 Years
• Maximum Eligible Age: 65 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Uşak University

OTHER

Sponsor Role: lead

Responsible Party

Ahu Dikilitaş

Usak University Faculty of Dentistry

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

ahu dikilitaş

Role: PRINCIPAL_INVESTIGATOR

University of Usak

fatih karaaslan

Role: STUDY_CHAIR

University of Usak

abdullah seçkin ertuğrul

Role: STUDY_CHAIR

University of IZMIR KATİP CELEBİ

Locations

University of Usak

Uşak, , Turkey (Türkiye)

Countries

Turkey (Türkiye)

Other Identifiers

UsakU7

Identifier Type: -

Identifier Source: org_study_id