Analysis of Gingival Crevicular Fluid

NCT ID: NCT05325905

Last Updated: 2022-04-13

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 81 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2014-10-31
• Study Completion Date: 2017-01-31

Brief Summary

The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.

Detailed Description

Gingival crevicular fluid (GCF) is formed when fluid exudes from the vessels of the microcirculation into the inflamed periodontal tissue and into the sulcus or pocket. As the fluid traverses the inflamed tissue, it is thought to pick up enzymes and other molecules that participate in the destructive process, as well as products of cell and tissue degradation. Efforts to develop diagnostic tests based on host factors have been focused almost entirely on analysis of GCF.

The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.

Systemically healthy and non-smokers 80 participants (30 males and 50 females) who were selected from among admitting to Dentistry Faculty of Selcuk University were included in this study. Clinical periodontal parameters were recorded and GCF samples were obtained. Samples were stored in -80 ˚C until analysis with Enzyme-Linked ImmunoSorbent Assay (ELISA).

Conditions

Periodontal Diseases

Study Design

• Observational Model Type: OTHER
• Study Time Perspective: CROSS_SECTIONAL

Study Groups

Healthy

bleeding on probing (BoP) ≤ 10% of the sites, probing depth (PD) ≤ 3 mm and no indication of clinical attachment level (CAL) or radiographic bone loss

Gingival crevicular fluid sampling

Intervention Type: DIAGNOSTIC_TEST

GCF sampling and clinical periodontal measurements were performed

Gingivitis

gingival inflammation (BoP ≥ 10% of the sites, red-blue discoloration of the gingiva and edema) PD ≤ 4 mm and no clinical attachment or radiographic bone loss

Gingival crevicular fluid sampling

Intervention Type: DIAGNOSTIC_TEST

GCF sampling and clinical periodontal measurements were performed

Periodontitis

PD ≤ 5 mm, radiographic bone loss, and 3-4 mm CAL in at least 30% of the sites

Gingival crevicular fluid sampling

Intervention Type: DIAGNOSTIC_TEST

GCF sampling and clinical periodontal measurements were performed

Interventions

Gingival crevicular fluid sampling

GCF sampling and clinical periodontal measurements were performed

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

• Systemically healthy
• non-smokers,
• presence of at least 20 teeth in the mouth

Exclusion Criteria

• receiving periodontal therapy in the last 6 months;
• receiving antibiotics or anti-inflammatory drugs in the last 6 months;
• currently pregnant or breastfeeding
• using a removable partial denture and removable or fixed orthodontic appliances.

• Minimum Eligible Age: 20 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Selcuk University

OTHER

Sponsor Role: collaborator

Bezmialem Vakif University

OTHER

Sponsor Role: lead

Responsible Party

Mehtikar Gursel

Prof.Dr

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Mehtikar Gürsel

Role: PRINCIPAL_INVESTIGATOR

Bezmialem University

Other Identifiers

2014/01

Identifier Type: -

Identifier Source: org_study_id