Virological and Immunological Determinants of Arbovirus Infection in New Caledonia

NCT ID: NCT04619823

Last Updated: 2024-05-23

Basic Information

• Recruitment Status: RECRUITING
• Clinical Phase: NA
• Total Enrollment: 250 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2021-02-15
• Study Completion Date: 2026-02-15

Brief Summary

Arboviruses, diseases transmitted to humans by the bite of an insect vector, are a major public health problem, particularly in tropical and sub-tropical countries. In New Caledonia, dengue epidemics are recurrent and may be associated with the co-circulation of other arboviruses such as Zika or chikungunya.

The virological determinants which condition the occurrence of these epidemics may be linked to an increased vectorial competence of the vector mosquito Aedes aegypti for a particular viral isolate. In fact, the Aedes aegypti mosquito is infected by making a blood meal on a person infected with an arbovirus. The virus infects its digestive tract, then spreads throughout the mosquito's body until it reaches its salivary glands. The virus is then present in the saliva and will be injected into the human host during a new blood meal. Some viral variants are best transmitted by Aedes aegypti. In general, the study of this vectorial competence is carried out by experiments in the laboratory during which an artificial blood meal composed of mammalian blood (human, rabbit, etc.) is mixed with a viral stock. Carrying out deported blood meals during which blood collected from patients infected with an arbovirus is used to gorge mosquitoes makes it possible to place oneself in experimental conditions as close as possible to the natural cycle of transmission of arboviruses.

In the human host, cells of the myeloid lineage present in the peripheral blood constitute preferred targets of replication for arboviruses. At the same time, the peripheral blood cells of patients are activated in response to infection and secrete many soluble factors released into the blood of patients. The study of blood samples from patients infected with arboviruses is therefore of prime importance for understanding both the replicative mechanisms of arboviruses but also the immune response they induce.

Detailed Description

Collection of blood samples from adult patients with clinical signs suggestive of arbovirus

This study is a interventional study that present minimal risks and constraints.

This study will improve :

• the reliability of the results of vector competence experiments
• the understanding of the mechanisms of infection and replication of arboviruses
• the knowledge of the immune mechanisms involved in the response to infection by an arbovirus

Conditions

Arbovirus Infections; Zika; Dengue; Chikungunya

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

Patient infected by arboviruses

Group Type: OTHER

Blood sample collection

Intervention Type: OTHER

Blood sample collection Mosquito collection at home

Interventions

Blood sample collection

Blood sample collection Mosquito collection at home

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Major patient
• Patient with clinical signs suggestive of an arbovirus infection:

Fever And at least 2 clinical signs suggestive of arbovirus among which myalgia, retro-orbital pain, drop in platelets (if data available), digestive symptoms, etc.

• Appearance of clinical signs suggestive of an arbovirus infection in the 7 days preceding the blood sample.
• Patient informed of the performance of the research, the collection of data and biological samples
• Patient who has given their consent to participate in the research by authorizing the collection of data, the collection of an additional blood sample and the use of this sample

Exclusion Criteria

• Patient suffering from another identified virosis, an inflammatory disease or taking anti-inflammatory drugs
• Pregnant patient
• Patient returning from a trip within 15 days before the onset of the signs,
• Patient for whom the blood sample is incomplete or could not be performed
• Patient's condition, which, in the doctor's opinion, is incompatible with the additional sample required by the study
• Patient not having consented to participate in the research

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Institut Pasteur de Nouvelle-Calédonie

UNKNOWN

Sponsor Role: collaborator

Centre Hospitalier Territorial de Nouvelle-Calédonie

OTHER

Sponsor Role: collaborator

Institut Pasteur

INDUSTRY

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Myrielle Dupont-Rouzeyrol, PhD

Role: PRINCIPAL_INVESTIGATOR

Institut Pasteur de Nouvelle-Calédonie

Locations

Centre Hospitalier Territorial de Nouvelle-Calédonie

Dumbéa Sur Mer, , New Caledonia

Site Status: RECRUITING

Countries

New Caledonia

Central Contacts

Myrielle Dupont-Rouzeyrol, PhD

Role: CONTACT

mdupont@pasteur.nc

+687 27 75 30

Catherine Inizan, PhD

Role: CONTACT

cinizan@pasteur.fr

+687 27 26 66

Facility Contacts

Myrielle Dupont Rouzeyrol, PhD

Role: primary

mdupont@pasteur.nc

+687 27 75 30

Other Identifiers

2019-062

Identifier Type: -

Identifier Source: org_study_id