Effect of Smoking on Saliva Composition and the Development of Dental Erosion

NCT ID: NCT04208802

Last Updated: 2026-01-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

50 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-09-24

Study Completion Date

2022-07-29

Brief Summary

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The aim of this study is to investigate whether smoking is associated with changes in salivary composition and/or predisposition to erosion.

Healthy volunteers are observationally wearing an intraoral device with both bovine tooth specimens (enamel and dentin) and resin specimens twice for two hours each. Afterwards, specimens are eroded extraorally and calcium release into the acid is measured.

Total protein concentration and protein composition of the salivary pellicles on the resin samples are measured. Additionally, salivary parameters (unstimulated and stimulated saliva flow rate, pH, buffer capacity, total protein content and protein composition as well as concentration of inorganic calcium, phosphate, and fluoride) are measured.

Detailed Description

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Conditions

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Dental Erosion

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Smokers

Volunteers smoking at least 10 cigarettes per day

Use of fluoridated toothpaste

Intervention Type OTHER

Use of fluoridated toothpaste

Wearing of an intraoral device with bovine tooth samples

Intervention Type OTHER

Wearing of an intraoral device with bovine tooth samples

Wearing of an intraoral device with resin samples

Intervention Type OTHER

Wearing of an intraoral device with resin samples

Non-smokers

Non-smoking volunteers

Use of fluoridated toothpaste

Intervention Type OTHER

Use of fluoridated toothpaste

Wearing of an intraoral device with bovine tooth samples

Intervention Type OTHER

Wearing of an intraoral device with bovine tooth samples

Wearing of an intraoral device with resin samples

Intervention Type OTHER

Wearing of an intraoral device with resin samples

Interventions

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Use of fluoridated toothpaste

Use of fluoridated toothpaste

Intervention Type OTHER

Wearing of an intraoral device with bovine tooth samples

Wearing of an intraoral device with bovine tooth samples

Intervention Type OTHER

Wearing of an intraoral device with resin samples

Wearing of an intraoral device with resin samples

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Volunteers aged between 20 and 50 years who are able to give written consent

* Intake of medications or diseases altering salivary secretion
* Refusal to use fluoridated toothpastes
* Known allergies to substances used in the study
* Orthodontic treatment or malfunction which does not allow wearing an intraoral device
Minimum Eligible Age

20 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Göttingen

OTHER

Sponsor Role lead

Responsible Party

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Philipp Kanzow, Prof. Dr. med. dent., Dr. rer. medic.

Senior Dental Practitioner

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Annette Wiegand, Prof. Dr. med. dent.

Role: PRINCIPAL_INVESTIGATOR

Dept. of Prev. Dentistry, Periodontology and Cariology, University Medical Center Göttingen, Germany

Locations

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University Medical Center Göttingen, Dept. of Preventive Dentistry, Periodontology and Cariology

Göttingen, Lower Saxony, Germany

Site Status

Countries

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Germany

References

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Carpenter G, Cotroneo E, Moazzez R, Rojas-Serrano M, Donaldson N, Austin R, Zaidel L, Bartlett D, Proctor G. Composition of enamel pellicle from dental erosion patients. Caries Res. 2014;48(5):361-7. doi: 10.1159/000356973. Epub 2014 Mar 6.

Reference Type BACKGROUND
PMID: 24603346 (View on PubMed)

Moazzez R, Bartlett D. Intrinsic causes of erosion. Monogr Oral Sci. 2014;25:180-96. doi: 10.1159/000360369. Epub 2014 Jun 26.

Reference Type BACKGROUND
PMID: 24993266 (View on PubMed)

Wiegand A, Bliggenstorfer S, Magalhaes AC, Sener B, Attin T. Impact of the in situ formed salivary pellicle on enamel and dentine erosion induced by different acids. Acta Odontol Scand. 2008 Aug;66(4):225-30. doi: 10.1080/00016350802183401.

Reference Type BACKGROUND
PMID: 18607835 (View on PubMed)

Wiegand A, Meier W, Sutter E, Magalhaes AC, Becker K, Roos M, Attin T. Protective effect of different tetrafluorides on erosion of pellicle-free and pellicle-covered enamel and dentine. Caries Res. 2008;42(4):247-54. doi: 10.1159/000135669. Epub 2008 Jun 4.

Reference Type BACKGROUND
PMID: 18523383 (View on PubMed)

Zwier N, Huysmans MC, Jager DH, Ruben J, Bronkhorst EM, Truin GJ. Saliva parameters and erosive wear in adolescents. Caries Res. 2013;47(6):548-52. doi: 10.1159/000350361. Epub 2013 Jun 15.

Reference Type BACKGROUND
PMID: 23774653 (View on PubMed)

Kanzow P, Wegehaupt FJ, Attin T, Wiegand A. Etiology and pathogenesis of dental erosion. Quintessence Int. 2016 Apr;47(4):275-8. doi: 10.3290/j.qi.a35625.

Reference Type BACKGROUND
PMID: 27022647 (View on PubMed)

Wiegand A, Rosemann A, Hoch M, Barke S, Dakna M, Kanzow P. Erosion-Protective Capacity of the Salivary Pellicle of Female and Male Subjects Is Not Different. Caries Res. 2019;53(6):636-642. doi: 10.1159/000500046. Epub 2019 Jun 4.

Reference Type BACKGROUND
PMID: 31163440 (View on PubMed)

Kanzow P, Lenz C, Schlarmann F, Barke S, Rohland B, Schmidt A, Gorshkova A, Neuenroth L, Wiegand A. Saliva and salivary pellicle composition and proteomic profile in smokers vs. non-smokers and its effect on dental erosion. J Dent. 2026 Jan 21:106517. doi: 10.1016/j.jdent.2026.106517. Online ahead of print.

Reference Type RESULT
PMID: 41577165 (View on PubMed)

Other Identifiers

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Erosion-Smoking

Identifier Type: -

Identifier Source: org_study_id

15/10/19

Identifier Type: OTHER

Identifier Source: secondary_id

02785

Identifier Type: OTHER

Identifier Source: secondary_id

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