Analysis of Circulating Tumor Markers in Blood

NCT ID: NCT04025541

Last Updated: 2025-04-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

992 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-05-29

Study Completion Date

2029-05-29

Brief Summary

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The circulating tumoral biomarkers in the blood are the object of numerous researches for several decades. The potential clinical interests of these circulating biomarkers are diagnostic, prognostic, predictive of the efficiency of targeted therapies (according to the mutational profile of the cancer), and could allow the study of the mechanisms of resistance under process. In the multiplicity of these blood potential biomarkers joins a permanent evolution of the technological means used to detect them/to quantify, as well as to estimate their clinical utility.

Detailed Description

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The new major challenge in the research concerns the circulating biomarkers, which aim at replacing the molecular analyses on tumour tissue obtained by biopsy (for example the search for somatic mutations of cancer) by a simple blood test (liquid biopsy). The other current important challenge is to have an idea of the interest to analyse the kinetics of blood markers, in particular in answer to a clinical "event", either through the chemotherapy, a biopsy and / or surgery. There is almost no data in the literature on this aspect. It is very likely that the liberation in the blood of the blood tumoral markers is strongly dependent on medical interventions on the tumour.

The study ALCINA 2 rests exactly on the principle of small cohorts, which correspond each to a clinical situation and/or a technique of different implemented detection, so as to generate data of feasibility and proof of concept. In case of success, statistical hypotheses will be necessary for the implementation of wider studies (being then the object of a specific approval by competent authorities).

Conditions

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Cancer Breast Cancer Sarcoma Lung Cancers Glioma Colon Cancer

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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COHORT 1 BREAST TUMOR/PALBOCICLIB

Patient with a locally Advanced tumor or metastatic tumor RH+/HER 2 - , treated by palbociclib

BLOOD SAMPLING

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

blood sampling time : cycle 1 day 15 and cycle 2 day 15 : one sample (6ml) by time

COHORT 2 BREAST TUMOR / RIBOCICLIB

Patient with a locally Advanced tumor or metastatic tumor RH+/HER 2 - , treated by ribociclib

BLOOD SAMPLING

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

blood sampling time : cycle 1 day 15 and cycle 2 day 15 : one sample (6ml) by time

COHORT 3 - LUNG CANCER

Patients with histologically proven metastatic bronchial carcinoma eligible for immunotherapy

Group Type OTHER

Blood sampling C3

Intervention Type BIOLOGICAL

Blood samples will be collected at four key time points:

* baseline (T1),
* first scan assessment (T2),
* second scan assessment (T3),
* and progression (T4).

COHORT 4 - CCRm

Patient with metastatic colorectal adenocarcinoma

Group Type OTHER

Blood sampling C4/7/10/13

Intervention Type BIOLOGICAL

Blood samples will be collected before any treatment

COHORT 5 - T-DXd

Patient with HER2 + metastatic breast cancer, requiring treatment with T-DXd

Group Type OTHER

Blood sampling C5

Intervention Type BIOLOGICAL

Blood samples will be collected at four key time points:

* At the inclusion (T1)
* Before the beginning of the treatment (cycle 1 day 1) (T2)
* After the first cycle of T-DXd (cycle 2 day 1) (T3)
* At progression or at the end of the follow-up (after 3 years) (T4)

COHORT 6 - Glioma

Patient with grade II, III or IV diffuse glioma

Group Type OTHER

Blood sampling C6

Intervention Type BIOLOGICAL

Blood samples will be collected at three key time points:

* At the inclusion (T1)
* For patients starting treatment at the time of inclusion (T2):
* Chemotherapy: 3 months after inclusion,
* Concurrent chemoradiotherapy with Temozolomide: 4-6 weeks after completion of radiotherapy,
* For patients starting treatment at the time of inclusion: at the time of tumor progression if occurring within one year of inclusion, or 12 months after inclusion in the absence of progression (T3).

COHORT 7 - CIRCUS 2

Patients with non-metastatic colon cancer

Group Type OTHER

Blood sampling C4/7/10/13

Intervention Type BIOLOGICAL

Blood samples will be collected before any treatment

COHORT 8 - CTC-AXL Breast

Patients with treatment-naive metastatic breast cancer with distant metastases

Group Type OTHER

Blood sampling C8

Intervention Type BIOLOGICAL

Blood samples will be collected at five key time points:

* At the inclusion
* At follow-up visit 2 to 6, every 3 months

COHORT 9 - ImmunoTNBC

Patients newly diagnosed with non-metastatic stage II - III early TNBC, requiring neoadjuvant treatment and previously untreated.

Group Type OTHER

Blood sampling C9

Intervention Type BIOLOGICAL

Blood samples will be collected at four key time points:

* At the inclusion before the beginning of the treatment (Cycle 1 Day 1)
* After the first cycle of the first chemo-immunotherapy sequence (Cycle 2 Day 1)
* After the first cycle of the second chemotherapy sequence (Cycle 2b Day 1)
* After the end of the whole neo-adjuvant chemo-immunotherapy protocol, before surgery

COHORT 10 - LPS

Patients with well differentiated (WD) liposarcoma, dedifferentiated (DD) liposarcoma or sarcoma other than liposarcoma

Group Type OTHER

Blood sampling C4/7/10/13

Intervention Type BIOLOGICAL

Blood samples will be collected before any treatment

COHORT 11 - LUNG DRIVER

Patients with Metastatic bronchial carcinoma activating alterations in EGFR (del 19; L858R) or KRAS G12C

Group Type OTHER

Blood sampling C11 + FFPE

Intervention Type BIOLOGICAL

Blood samples will be collected at five key time points:

* At the inclusion (T1)
* At first clinical evaluation (T2): 4th week after start of treatment
* At first scan evaluation (T3a): 8th week after start of treatment
* At the Nth scan evaluation (T3b, c, ...)
* At progression (T4)

Tumor sampling :

* At the inclusion
* At tumor progression

COHORT 12 - LMD

Patient with breast cancer and suspected with leptomeningeal metastases

Group Type OTHER

Blood sampling C12

Intervention Type BIOLOGICAL

Blood samples will be collected at several points :

* Inclusion: at the time of suspected leptomeningeal metastases, prior to any specific treatment,
* Every 4 weeks until meningeal progression, or for a maximum of 4 months,
* Then every 3 months beyond 4 months until meningeal progression.

COHORT 13 - RILA STAB

Patients with breast cancer requiring radiotherapy, whatever the tumor stage

Group Type OTHER

Blood sampling C4/7/10/13

Intervention Type BIOLOGICAL

Blood samples will be collected before any treatment

Interventions

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Blood sampling

blood sampling time : cycle 1 day 15 and cycle 2 day 15 : one sample (6ml) by time

Intervention Type BIOLOGICAL

Blood sampling C3

Blood samples will be collected at four key time points:

* baseline (T1),
* first scan assessment (T2),
* second scan assessment (T3),
* and progression (T4).

Intervention Type BIOLOGICAL

Blood sampling C4/7/10/13

Blood samples will be collected before any treatment

Intervention Type BIOLOGICAL

Blood sampling C5

Blood samples will be collected at four key time points:

* At the inclusion (T1)
* Before the beginning of the treatment (cycle 1 day 1) (T2)
* After the first cycle of T-DXd (cycle 2 day 1) (T3)
* At progression or at the end of the follow-up (after 3 years) (T4)

Intervention Type BIOLOGICAL

Blood sampling C6

Blood samples will be collected at three key time points:

* At the inclusion (T1)
* For patients starting treatment at the time of inclusion (T2):
* Chemotherapy: 3 months after inclusion,
* Concurrent chemoradiotherapy with Temozolomide: 4-6 weeks after completion of radiotherapy,
* For patients starting treatment at the time of inclusion: at the time of tumor progression if occurring within one year of inclusion, or 12 months after inclusion in the absence of progression (T3).

Intervention Type BIOLOGICAL

Blood sampling C8

Blood samples will be collected at five key time points:

* At the inclusion
* At follow-up visit 2 to 6, every 3 months

Intervention Type BIOLOGICAL

Blood sampling C9

Blood samples will be collected at four key time points:

* At the inclusion before the beginning of the treatment (Cycle 1 Day 1)
* After the first cycle of the first chemo-immunotherapy sequence (Cycle 2 Day 1)
* After the first cycle of the second chemotherapy sequence (Cycle 2b Day 1)
* After the end of the whole neo-adjuvant chemo-immunotherapy protocol, before surgery

Intervention Type BIOLOGICAL

Blood sampling C11 + FFPE

Blood samples will be collected at five key time points:

* At the inclusion (T1)
* At first clinical evaluation (T2): 4th week after start of treatment
* At first scan evaluation (T3a): 8th week after start of treatment
* At the Nth scan evaluation (T3b, c, ...)
* At progression (T4)

Tumor sampling :

* At the inclusion
* At tumor progression

Intervention Type BIOLOGICAL

Blood sampling C12

Blood samples will be collected at several points :

* Inclusion: at the time of suspected leptomeningeal metastases, prior to any specific treatment,
* Every 4 weeks until meningeal progression, or for a maximum of 4 months,
* Then every 3 months beyond 4 months until meningeal progression.

Intervention Type BIOLOGICAL

Other Intervention Names

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Tumor sampling

Eligibility Criteria

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Inclusion Criteria

1. Patient presenting an invasive tumoral pathology (proved or suspected), whatever is the location or the stage,
2. Man or woman ≥ 18 years,
3. Obtaining of the informed consent signed before any procedure of specific preselection on approval.

Exclusion Criteria

1. Private persons of freedom or under guardianship,
2. Patient whose regular follow-up is impossible for psychological, family, social or geographical reasons,
3. Pregnant woman and/or breast-feeding,
4. Unaffiliated patient to Social Protection System,
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Institut du Cancer de Montpellier - Val d'Aurelle

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Centre Regional de Lutte Contre le Cancer - Centre Val d'Aurelle

Montpellier, , France

Site Status RECRUITING

ICM

Montpellier, , France

Site Status RECRUITING

Countries

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France

Central Contacts

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texier emmannuelle

Role: CONTACT

0467613102

Facility Contacts

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Marc Ychou, MD, PhD

Role: primary

33-4-6761-3066

Aurore MOUSSION

Role: backup

0467613102 ext. +33

WILLIAM JACOT

Role: primary

References

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Bardol T, Eslami-S Z, Masmoudi D, Alexandre M, Duboys de Labarre M, Bobrie A, D'Hondt V, Guiu S, Kurma K, Cayrefourcq L, Jacot W, Alix-Panabieres C. First evidence of AXL expression on circulating tumor cells in metastatic breast cancer patients: A proof-of-concept study. Cancer Med. 2024 Jan;13(1):e6843. doi: 10.1002/cam4.6843. Epub 2023 Dec 22.

Reference Type BACKGROUND
PMID: 38132919 (View on PubMed)

Leenhardt F, Fiteni F, Gauthier L, Alexandre M, Guiu S, Firmin N, Pouderoux S, Viala M, Lossaint G, Gautier C, Mollevi C, Gracia M, Gongora C, Mbatchi L, Evrard A, Jacot W. Pharmacokinetic Variability Drives Palbociclib-Induced Neutropenia in Metastatic Breast Cancer Patients: Drug-Drug Interactions Are the Usual Suspects. Pharmaceutics. 2022 Apr 11;14(4):841. doi: 10.3390/pharmaceutics14040841.

Reference Type BACKGROUND
PMID: 35456675 (View on PubMed)

Leenhardt F, Alexandre M, Guiu S, Pouderoux S, Beaujouin M, Lossaint G, Philibert L, Evrard A, Jacot W. Impact of pharmacist consultation at clinical trial inclusion: an effective way to reduce drug-drug interactions with oral targeted therapy. Cancer Chemother Pharmacol. 2021 Oct;88(4):723-729. doi: 10.1007/s00280-021-04331-0. Epub 2021 Jul 20.

Reference Type BACKGROUND
PMID: 34286354 (View on PubMed)

Leenhardt F, Gracia M, Perrin C, Muracciole-Bich C, Marion B, Roques C, Alexandre M, Firmin N, Pouderoux S, Mbatchi L, Gongora C, Jacot W, Evrard A. Liquid chromatography-tandem mass spectrometric assay for the quantification of CDK4/6 inhibitors in human plasma in a clinical context of drug-drug interaction. J Pharm Biomed Anal. 2020 Sep 5;188:113438. doi: 10.1016/j.jpba.2020.113438. Epub 2020 Jun 22.

Reference Type BACKGROUND
PMID: 32623316 (View on PubMed)

Other Identifiers

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PROICM 2017-05 BAL

Identifier Type: -

Identifier Source: org_study_id

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