Analysis of Circulating Tumor Markers in the Blood (ALCINA)

NCT ID: NCT02866149

Last Updated: 2024-10-01

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

682 participants

Study Classification

INTERVENTIONAL

Study Start Date

2015-07-31

Study Completion Date

2024-09-30

Brief Summary

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Exploratory study on blood-borne biological markers and their correlation with clinical and pathological characteristics.

Detailed Description

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Exploratory multi-cohort study including different types of cancer (different organs and/or different histological types).

Each kind of blood-borne biological markers analyses corresponds to a cohort.

Conditions

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Cancer

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Cohort 1 - "Anti checkpoint"

Monitoring of patients with tumours treated by immune therapy.

Timing of blood sampling:

* inclusion
* after #8 weeks on therapy
* at progression or 6 months from inclusion for patient without progressive disease
* if toxicity grade 3 or 4, or grade 2 until 1 month.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 2 - "Oncoscan®"

Monitoring of patients with HER 2+/- breast cancer and correlation with genome-wide copy number, loss of heterozygosity detection, as well as identification of frequently tested somatic mutations (Oncoscan® assays).

Timing of blood sampling:

* inclusion
* after 1 cycle of therapy (weeks 3-4)
* up to 2 other samples, timepoints decided by the investigator

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 3 - "CirCe-PLA"

Feasibility of Proximity-Ligation Assay (PLA) to study membrane proteins dimerisation by on isolated tumour cells in patients with HER2+/- breast cancer (HER 2+/-).

One tumor sampling.

Timing of blood sampling:

* Inclusion
* up to 3 other samples, timepoints decided by the investigator.

Group Type EXPERIMENTAL

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Tumor sampling

Intervention Type PROCEDURE

One tumor sampling can be performed, if applicable

Cohort 4 - "CDX PDX"

Establishment of xenografts from tumor (PDX) and from Circulating Tumour Cell (CDX) by tumour and blood sampling.

One tumor sampling.

Timing of blood sampling:

* Inclusion
* up to 3 other samples, timepoints decided by the investigator.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Tumor sampling

Intervention Type PROCEDURE

One tumor sampling can be performed, if applicable

Cohort 5 - "Post-TP53"

Follow-up of patients previously treated by neoadjuvant chemotherapy for triple negative breast cancer.

Timing of blood sampling:

* Inclusion
* up to 3 other samples, timepoints decided by the investigator.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 6 - "Palbociclib"

Monitoring of patients treated with palbociclib

Timing of blood sampling:

* Inclusion day (2 samples)
* after #2 weeks of therapy
* after #4 weeks of therapy
* at progression.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 7 - "CTC_PD-L1_Breast"

Detection of PD-L1 in metastatic breast cancer patients

Timing of blood sampling:

* Inclusion
* up to 3 other samples, timepoints decided by the investigator.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 8 - "CTC_PD-L1_Broncho-Pulmonary"

Detection of PD-L1 in metastatic lung cancer patients

Timing of blood sampling:

* Inclusion
* up to 3 other samples, timepoints decided by the investigator.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 9 - "NSCLC"

Monitoring of patients with Non-Small Cell lung Cancer treated by immune therapy.

Blood sampling at 4 timepoints.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Tumor sampling

Intervention Type PROCEDURE

One tumor sampling can be performed, if applicable

Stool sampling

Intervention Type OTHER

Up to 5 blood samplings can be performed at different time points

Cohort 10 - "Palbociclib II"

Monitoring of patient with a metastatic breast cancer treated by palbociclib.

Timing of blood sampling:

* Inclusion
* after #4 weeks of therapy
* at the first tumoral evaluation (month 3 or 4)
* at progression.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 11 - Sarcomas

The cohort includes all patients with bone or soft tissue sarcoma. Timing of blood sampling depending on disease staging.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 12 - Faslorad

Monitoring of patient with a metastatic breast cancer initiating a treatment by Faslodex-Afinitor.

Timing of blood sampling:

* Inclusion
* after #3-5 weeks of therapy
* at the first tumoral evaluation (month 2 or 3)
* at progression.

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 13 - MUm

The cohort concerns patients with uveal melanoma in the 1st systemic line at the metastatic stage (may have had prior adjuvant therapy or surgery/radiofrequency).

Timing of blood sampling:

* J1C1
* J2C1
* J1C2
* J1C5 (first tumoral evaluation).

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Cohort 14 - CNBC Snipe

This cohort concerns patients with metastatic Non-Small Cell lung Cancer receiving anti-PD-1/PD-L1.

One tumour sampling.

Timing of blood sampling:

* before treatment
* at W8 of treatment (after radiological examination)
* at W12 of treatment
* at progression or 18 months after the beginning of treatment

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Tumor sampling

Intervention Type PROCEDURE

One tumor sampling can be performed, if applicable

Cohort 15 - Breast CLI

This cohort concerns patients with metastatic lobular breast cancer One tumour sampling.

Timing of blood sampling:

* At inclusion
* After biopsy post inclusion (or in 15 days after)
* after 1 or 2 months of treatment
* at progression or 18 months after inclusion

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Tumor sampling

Intervention Type PROCEDURE

One tumor sampling can be performed, if applicable

Cohort 16 - Mum immunothérapie

This cohort concerns patients with metastatic uveal melanoma before immunotherapy treatment.

Timing of blood sampling :

* at inclusion
* at cycle 2 or 3 of treatment
* at the first tumoral evaluation (C5D1)
* at progression

Group Type OTHER

Blood sampling

Intervention Type BIOLOGICAL

Up to 5 blood samplings can be performed at different time points

Interventions

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Blood sampling

Up to 5 blood samplings can be performed at different time points

Intervention Type BIOLOGICAL

Tumor sampling

One tumor sampling can be performed, if applicable

Intervention Type PROCEDURE

Stool sampling

Up to 5 blood samplings can be performed at different time points

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

1. Patient with any tumoral disease (proven or suspected), of any type and stage
2. More than18 years old
3. Signed informed consent form

4. Tumor considered as accessible by biopsy
5. Normal blood coagulation tests on the last blood analysis


1. Patient in detention or protected by the law
2. Patient who cannot comply with the study follow up for geographical, social or psychological reasons

3. Anticoagulant or antiaggregant that cannot be interrupted for the biopsy
4. central-nervous system metastases only (unless a diagnostic or curative surgery is planned before the inclusion in the study)
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Institut Curie

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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François-Clément BIDARD, MD PhD

Role: STUDY_DIRECTOR

Institut Curie, Paris (FR)

Locations

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Centre Georges François Leclerc

Dijon, , France

Site Status

Institut du Cancer de Montpellier

Montpellier, , France

Site Status

Institut Curie (Paris hospital)

Paris, , France

Site Status

Institut Mutualiste Montsouris

Paris, , France

Site Status

Institut Curie (St Cloud hospital)

Saint-Cloud, , France

Site Status

Countries

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France

References

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Rodrigues M, Ramtohul T, Rampanou A, Sandoval JL, Houy A, Servois V, Mailly-Giacchetti L, Pierron G, Vincent-Salomon A, Cassoux N, Mariani P, Dutriaux C, Pracht M, Ryckewaert T, Kurtz JE, Roman-Roman S, Piperno-Neumann S, Bidard FC, Stern MH, Renault S. Prospective assessment of circulating tumor DNA in patients with metastatic uveal melanoma treated with tebentafusp. Nat Commun. 2024 Oct 14;15(1):8851. doi: 10.1038/s41467-024-53145-0.

Reference Type DERIVED
PMID: 39402032 (View on PubMed)

Eslami-S Z, Cortes-Hernandez LE, Sinoquet L, Gauthier L, Vautrot V, Cayrefourcq L, Avoscan L, Jacot W, Pouderoux S, Viala M, Thomas QD, Lamy PJ, Quantin X, Gobbo J, Alix-Panabieres C. Circulating tumour cells and PD-L1-positive small extracellular vesicles: the liquid biopsy combination for prognostic information in patients with metastatic non-small cell lung cancer. Br J Cancer. 2024 Jan;130(1):63-72. doi: 10.1038/s41416-023-02491-9. Epub 2023 Nov 16.

Reference Type DERIVED
PMID: 37973956 (View on PubMed)

Sinoquet L, Jacot W, Gauthier L, Pouderoux S, Viala M, Cayrefourcq L, Quantin X, Alix-Panabieres C. Programmed Cell Death Ligand 1-Expressing Circulating Tumor Cells: A New Prognostic Biomarker in Non-Small Cell Lung Cancer. Clin Chem. 2021 Nov 1;67(11):1503-1512. doi: 10.1093/clinchem/hvab131.

Reference Type DERIVED
PMID: 34355741 (View on PubMed)

Darrigues L, Pierga JY, Bernard-Tessier A, Bieche I, Silveira AB, Michel M, Loirat D, Cottu P, Cabel L, Dubot C, Geiss R, Ricci F, Vincent-Salomon A, Proudhon C, Bidard FC. Circulating tumor DNA as a dynamic biomarker of response to palbociclib and fulvestrant in metastatic breast cancer patients. Breast Cancer Res. 2021 Mar 6;23(1):31. doi: 10.1186/s13058-021-01411-0.

Reference Type DERIVED
PMID: 33676547 (View on PubMed)

Cabel L, Rosenblum D, Lerebours F, Brain E, Loirat D, Bergqvist M, Cottu P, Donnadieu A, Bethune A, Kiavue N, Rodrigues M, Pierga JY, Tanguy ML, Bidard FC. Plasma thymidine kinase 1 activity and outcome of ER+ HER2- metastatic breast cancer patients treated with palbociclib and endocrine therapy. Breast Cancer Res. 2020 Sep 14;22(1):98. doi: 10.1186/s13058-020-01334-2.

Reference Type DERIVED
PMID: 32928264 (View on PubMed)

Jacot W, Mazel M, Mollevi C, Pouderoux S, D'Hondt V, Cayrefourcq L, Bourgier C, Boissiere-Michot F, Berrabah F, Lopez-Crapez E, Bidard FC, Viala M, Maudelonde T, Guiu S, Alix-Panabieres C. Clinical Correlations of Programmed Cell Death Ligand 1 Status in Liquid and Standard Biopsies in Breast Cancer. Clin Chem. 2020 Aug 1;66(8):1093-1101. doi: 10.1093/clinchem/hvaa121.

Reference Type DERIVED
PMID: 32712650 (View on PubMed)

Other Identifiers

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IC 2015-02

Identifier Type: -

Identifier Source: org_study_id

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