Transcriptome Assessment After Cardiac Arrest

NCT ID: NCT03895736

Last Updated: 2025-11-20

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 60 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2020-02-24
• Study Completion Date: 2022-02-10

Brief Summary

The purpose of this study is to determine whether the blood transcriptome of patients resuscitated after out-of-hospital could be an early predictor of the neurological outcome.

Detailed Description

Assessing the prognosis of the patients resuscitated after out-of-hospital cardiac arrest (OHCA) is still highly challenging. Beyond usual neurological markers, experimental and clinical studies have also shown that blood levels of several cytokines or miRNA could be significantly different after cardiac arrest in subjects prematurely died as compared to survivors with a good neurological recovery. Here the investigators propose to investigate a screening approach assessing simultaneously multiple targets of the post-cardiac arrest syndrome through analysis of the whole transcriptome of patients after OHCA. For this purpose, the investigators will collect blood samples from 60 patients after admission at hospital and then 1 and 3 days later. The Pittsburgh Cerebral Performance Category of each patient will be evaluated for each patient at day 60 after cardiac arrest. At the end of the collection period, the blood samples will be prepared for whole genome analysis and RNA sequencing. The primary analysis of the investigators will be the comparison of the transcriptome at day 0 after cardiac arrest in patients reaching Cerebral Performance Category (CPC) 1 at day 60 as compared to all other patients.

Conditions

Out-Of-Hospital Cardiac Arrest After Initial Successful Resuscitation

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: DIAGNOSTIC
• Blinding Strategy: NONE

Interventions

blood sampling

Sampling of 15 ml of blood during a blood test at Day 0, Day 1 and Day 3

Intervention Type: BIOLOGICAL

Other Intervention Names

Blood sampling at Day 0, Day 1 and Day 3

Eligibility Criteria

Inclusion Criteria

• Age between 18 and 80 years
• Delay between patients collapse and resumption of spontaneous circulation (ROSC) < 60 minutes
• Comatose patients ROSC with Glasgow score < 7
• Patient already registered in the social security system
• Consent of a family member, if able to understand in the emergency context, or attestation of emergency situation with a follow-up information notice

Exclusion Criteria

• Cardiac arrest supposed to be provoked by trauma or sepsis

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 80 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

National Research Agency, France

OTHER

Sponsor Role: collaborator

University of Paris 5 - Rene Descartes

OTHER

Sponsor Role: collaborator

Institut National de la Santé Et de la Recherche Médicale, France

OTHER_GOV

Sponsor Role: collaborator

Paris 12 Val de Marne University

OTHER

Sponsor Role: collaborator

Ecole Nationale Vétérinaire d'Alfort

UNKNOWN

Sponsor Role: collaborator

URC-CIC Paris Descartes Necker Cochin

OTHER

Sponsor Role: collaborator

Assistance Publique - Hôpitaux de Paris

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Alain Cariou, Prof.

Role: PRINCIPAL_INVESTIGATOR

Assistance Publique - Hôpitaux de Paris

Renaud Tissier, Prof

Role: STUDY_CHAIR

Ecole Nationale Vétérinaire d'Alfort

Locations

Hôpital Cochin, Assistance Publique Hôpitaux de Paris

Paris, , France

Countries

France

Other Identifiers

2019-A00239-48

Identifier Type: REGISTRY

Identifier Source: secondary_id

2019-A00239-48

Identifier Type: -

Identifier Source: org_study_id