To Evaluate the Effect of Reveratrol,Folic Acid, Vitamin D, Vitamin B12 and B6 in Male Infertility

NCT ID: NCT03864198

Last Updated: 2019-03-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

20 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-01-20

Study Completion Date

2019-04-30

Brief Summary

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The objective of this study is to evaluate the impact of the Genante(TM) on the spermiogram parameters in infertile male patients

Detailed Description

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This is a single-centre prospective observational study. Twenty infertile male patients.

Inclusion criteria are:

Age:18-50 years oligozoospermia: \< 5 million spermatozoa/mL; asthenozoospermia: \< 32% progressive motile spermatozoa; teratozoospermia: \< 4% normal forms.

Esclusion criteria:

Patients with azoospermia, who smoke, use drugs, drugs with proven fertility toxicity, exposed to any environmental or occupational toxic substances, radiation or heat; with orchitis secondary to mumps, sexually transmitted diseases, history of cryptorchidism, previous testicular torsion, genitourinary anomalies, alterations of the epididymis or deferens; and inguinal surgery

Pretreatment evaluation included:

Semen analysis Hormonal determinations:follicle-stimulating hormone (FSH) and luteinising hormone (LH) total Testosterone Total,estradiol, prolactina, 25-OH-Vitamin D3); scrotal ultrasound prostatic transrectal ultrasound

Outocmes measures (baseline, at 1 and 6 months):

* Semen volume (mL)
* Total sperm number (106/ejaculate)
* Sperm concentration (106/mL)
* Total motility (PR + NP)
* Progressive motility (PR, %)
* Vitality (live spermatozoa, %)
* Sperm morphology (normal forms, %)
* Ph
* FSH
* LH
* Testosterone totale
* Estradiol
* Prolactiona
* 25-OH-Vitamin D3
* scrotal ultrasound
* prostatic transrectal ultrasound

Conditions

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Male Infertility

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Genante (Tm)

Genante tablets One tablet in the morning and one tablet in the evening for 3 months

Group Type OTHER

Genante

Intervention Type DIETARY_SUPPLEMENT

A dietary supplement for male infertility

Interventions

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Genante

A dietary supplement for male infertility

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* male 18-50 years
* oligozoospermia: \< 5 million spermatozoa/mL;
* asthenozoospermia: \< 32% progressive motile spermatozoa;
* teratozoospermia: \< 4% normal forms.

Exclusion Criteria

* azoospermia
* smoke
* use drugs
* use drugs with proven fertility toxicity
* exposition to any environmental or occupational toxic substances
* exposition to radiation or heat
* orchitis secondary to mumps
* sexually transmitted diseases
* history of cryptorchidism
* previous testicular torsion
* genitourinary anomalies
* alterations of the epididymis or deferens
* inguinal surgery
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Ester Illiano, MD

UNKNOWN

Sponsor Role collaborator

University Of Perugia

OTHER

Sponsor Role lead

Responsible Party

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Elisabetta Costantini

Clinical Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Ester Illiano, MD

Role: STUDY_DIRECTOR

University Of Perugia

Locations

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Elisabetta Costantini

Terni, , Italy

Site Status RECRUITING

Countries

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Italy

Central Contacts

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Elisabetta Costantini, Professor

Role: CONTACT

3283620614

Ester Illiano, MD

Role: CONTACT

3283620614

Facility Contacts

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Elisabetta Costantini, Professor

Role: primary

3283620614

Ester Illiano, MD

Role: backup

3283620614

References

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Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online. 2004 Jun;8(6):616-27. doi: 10.1016/s1472-6483(10)61641-0.

Reference Type BACKGROUND
PMID: 15169573 (View on PubMed)

Griveau JF, Le Lannou D. Reactive oxygen species and human spermatozoa: physiology and pathology. Int J Androl. 1997 Apr;20(2):61-9. doi: 10.1046/j.1365-2605.1997.00044.x.

Reference Type BACKGROUND
PMID: 9292315 (View on PubMed)

Aitken RJ. Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev. 1995;7(4):659-68. doi: 10.1071/rd9950659.

Reference Type BACKGROUND
PMID: 8711202 (View on PubMed)

de Lamirande E, Leclerc P, Gagnon C. Capacitation as a regulatory event that primes spermatozoa for the acrosome reaction and fertilization. Mol Hum Reprod. 1997 Mar;3(3):175-94. doi: 10.1093/molehr/3.3.175.

Reference Type BACKGROUND
PMID: 9237244 (View on PubMed)

Hughes CM, Lewis SE, McKelvey-Martin VJ, Thompson W. The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity. Hum Reprod. 1998 May;13(5):1240-7. doi: 10.1093/humrep/13.5.1240.

Reference Type BACKGROUND
PMID: 9647554 (View on PubMed)

Erben RG, Soegiarto DW, Weber K, Zeitz U, Lieberherr M, Gniadecki R, Moller G, Adamski J, Balling R. Deletion of deoxyribonucleic acid binding domain of the vitamin D receptor abrogates genomic and nongenomic functions of vitamin D. Mol Endocrinol. 2002 Jul;16(7):1524-37. doi: 10.1210/mend.16.7.0866.

Reference Type BACKGROUND
PMID: 12089348 (View on PubMed)

Hammoud AO, Meikle AW, Peterson CM, Stanford J, Gibson M, Carrell DT. Association of 25-hydroxy-vitamin D levels with semen and hormonal parameters. Asian J Androl. 2012 Nov;14(6):855-9. doi: 10.1038/aja.2012.77. Epub 2012 Oct 8.

Reference Type BACKGROUND
PMID: 23042450 (View on PubMed)

Wong WY, Merkus HM, Thomas CM, Menkveld R, Zielhuis GA, Steegers-Theunissen RP. Effects of folic acid and zinc sulfate on male factor subfertility: a double-blind, randomized, placebo-controlled trial. Fertil Steril. 2002 Mar;77(3):491-8. doi: 10.1016/s0015-0282(01)03229-0.

Reference Type BACKGROUND
PMID: 11872201 (View on PubMed)

Chen Q, Ng V, Mei J, Chia SE. [Comparison of seminal vitamin B12, folate, reactive oxygen species and various sperm parameters between fertile and infertile males]. Wei Sheng Yan Jiu. 2001 Mar;30(2):80-2. Chinese.

Reference Type BACKGROUND
PMID: 11321956 (View on PubMed)

Das S, Lin HS, Ho PC, Ng KY. The impact of aqueous solubility and dose on the pharmacokinetic profiles of resveratrol. Pharm Res. 2008 Nov;25(11):2593-600. doi: 10.1007/s11095-008-9677-1. Epub 2008 Jul 16.

Reference Type BACKGROUND
PMID: 18629618 (View on PubMed)

Amri A, Chaumeil JC, Sfar S, Charrueau C. Administration of resveratrol: What formulation solutions to bioavailability limitations? J Control Release. 2012 Mar 10;158(2):182-93. doi: 10.1016/j.jconrel.2011.09.083. Epub 2011 Sep 25.

Reference Type BACKGROUND
PMID: 21978644 (View on PubMed)

Orihuela-Campos RC, Tamaki N, Mukai R, Fukui M, Miki K, Terao J, Ito HO. Biological impacts of resveratrol, quercetin, and N-acetylcysteine on oxidative stress in human gingival fibroblasts. J Clin Biochem Nutr. 2015 May;56(3):220-7. doi: 10.3164/jcbn.14-129. Epub 2015 Mar 28.

Reference Type BACKGROUND
PMID: 26060353 (View on PubMed)

Liu J, Yi L, Xiang Z, Zhong J, Zhang H, Sun T. Resveratrol attenuates spinal cord injury-induced inflammatory damage in rat lungs. Int J Clin Exp Pathol. 2015 Feb 1;8(2):1237-46. eCollection 2015.

Reference Type BACKGROUND
PMID: 25973008 (View on PubMed)

Illiano E, Trama F, Zucchi A, Iannitti RG, Fioretti B, Costantini E. Resveratrol-Based Multivitamin Supplement Increases Sperm Concentration and Motility in Idiopathic Male Infertility: A Pilot Clinical Study. J Clin Med. 2020 Dec 11;9(12):4017. doi: 10.3390/jcm9124017.

Reference Type DERIVED
PMID: 33322606 (View on PubMed)

Other Identifiers

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3310

Identifier Type: REGISTRY

Identifier Source: secondary_id

RESV1

Identifier Type: -

Identifier Source: org_study_id

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