Effect of Branched-chain Amino Acid Supplementation on Muscle Damage

NCT ID: NCT03766815

Last Updated: 2019-10-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

78 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-10-01

Study Completion Date

2019-12-31

Brief Summary

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Seventy-eight recreational athletes who never resistance trained will be randomly assigned into three groups of twenty-six. Branched-chain amino acid will be supplemented for 18days at 0, 200 or 400 mg/body weight(kg)/day in jelly. Participants will be asked to perform elbow flexions on the 12th day of supplementation. Maximum voluntary contractions will be measured before, during and after the supplementation period to compare the effects of different doses of branched-chain amino acid has on muscle damage markers.

Detailed Description

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Previous studies have not been able to show consistent effects due to various reasons. The major limitations are the lack of enough data on the amount and duration of branched-chain amino acid supplementation, and the level of muscle damage for branched-chain amino acid to be effective. Based on a recent meta-analysis, it has been suggested that to be effective, branched-chain amino acid should be supplemented more than 200mg/kg/day before a low-to-moderate intensity exercise bout. Therefore, this study is designed based on the above suggestions and to compare the effect of different doses of branched-chain amino acid on muscle damage markers in healthy recreational active individuals.

Three treatment groups (n=26) will be randomly assigned to be supplemented with either 200mg BCAA/kg/day (BCAA200), 400mg BCAA/kg/day (BCAA400) or placebo (fiber supplement; PL). Supplements will be provided in jelly in a single blind fashion to be ingested 1hour after each of the three main daily meals for 18days. Anthropometric measurements (body weight, lean mass, height), body temperature, ultrasound (US), strength assessment (maximal voluntary contraction, MVC), resting metabolic rate (RMR), rate of perceived exertion (RPE), delayed onset of muscle soreness (DOMS) and blood samples (duplicates; myoglobin; BCAA) will be collected before, during and after the supplementation period at different time points (see appendix for the above tables). Repeated-bout effect (RBE) on both arms will be measured 7 days after the damage inducing session.

Conditions

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Muscle Damage

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Participants will be supplemented for 18 days with either 200mg BCAA/kg/day, 400mg BCAA/kg/day or placebo (fiber supplement) in jelly in a single-blind fashion. Followed by a muscle damaging exercise and subsequent MVC and blood sample for force and metabolite measurements.
Primary Study Purpose

OTHER

Blinding Strategy

SINGLE

Participants
Participants will not be able to distinguish branched-chain amino acid and fiber supplement by taste, color or scent as the supplements are provided in jelly.

Study Groups

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Placebo

Fiber supplement mixed with jelly will be ingested for 18 days with participants performing elbow eccentric contractions as a muscle damaging exercise on the 12th-day of supplementation.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type DIETARY_SUPPLEMENT

Fiber supplement jelly for 18 days.

Muscle damaging exercise

Intervention Type BEHAVIORAL

Participants will be asked to perform muscle damaging elbow eccentric contraction exercise at 120% of 1RM for three sets of 15 repetitions with the dominant arm at maximal effort on a Biodex machine with a 3 minute rest period between each set.

BCAA 200mg/kg/day

Branched-chain amino acid supplement mixed with jelly will be ingested for 18 days. The amount of supplement provided will be based on body weight (200mg/kg/day) with participants performing elbow eccentric contractions as a muscle damaging exercise on the 12th-day of supplementation.

Group Type EXPERIMENTAL

BCAA 200mg/kg/day

Intervention Type DIETARY_SUPPLEMENT

BCAA supplement based on 200mg/kg/day for 18 days.

Muscle damaging exercise

Intervention Type BEHAVIORAL

Participants will be asked to perform muscle damaging elbow eccentric contraction exercise at 120% of 1RM for three sets of 15 repetitions with the dominant arm at maximal effort on a Biodex machine with a 3 minute rest period between each set.

BCAA 400mg/kg/day

Branched-chain amino acid supplement mixed with jelly will be ingested for 18 days. The amount of supplement provided will be based on body weight (400mg/kg/day) with participants performing elbow eccentric contractions as a muscle damaging exercise on the 12th-day of supplementation.

Group Type EXPERIMENTAL

BCAA 400mg/kg/day

Intervention Type DIETARY_SUPPLEMENT

BCAA supplement based on 400mg/kg/day for 18 days.

Muscle damaging exercise

Intervention Type BEHAVIORAL

Participants will be asked to perform muscle damaging elbow eccentric contraction exercise at 120% of 1RM for three sets of 15 repetitions with the dominant arm at maximal effort on a Biodex machine with a 3 minute rest period between each set.

Interventions

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BCAA 200mg/kg/day

BCAA supplement based on 200mg/kg/day for 18 days.

Intervention Type DIETARY_SUPPLEMENT

Placebo

Fiber supplement jelly for 18 days.

Intervention Type DIETARY_SUPPLEMENT

BCAA 400mg/kg/day

BCAA supplement based on 400mg/kg/day for 18 days.

Intervention Type DIETARY_SUPPLEMENT

Muscle damaging exercise

Participants will be asked to perform muscle damaging elbow eccentric contraction exercise at 120% of 1RM for three sets of 15 repetitions with the dominant arm at maximal effort on a Biodex machine with a 3 minute rest period between each set.

Intervention Type BEHAVIORAL

Eligibility Criteria

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Inclusion Criteria

* Healthy males and females at the age of 18-40
* Endurance trained athletes
* Weekly exercise duration is less than 150minutes but more than 30 minutes

Exclusion Criteria

* Individuals who cannot endure three days for not eating meat
* Diabetic
* Have been performing resistance trainings
* Have been taking protein supplement
* Have been consuming a high level of protein in the regular diet
* Have been taking omega-three and vitamin E supplements
* Have been taking anabolic Steroids
* Have been taking regular medications
* Have cardiovascular Disease
* Have a history of joint \& Muscle Injuries
* Females with irregular Menstrual Cycle
Minimum Eligible Age

18 Years

Maximum Eligible Age

40 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Western University, Canada

OTHER

Sponsor Role lead

Responsible Party

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Peter Lemon

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Peter Lemon, PhD

Role: PRINCIPAL_INVESTIGATOR

Western University

Locations

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Exercise Nutrition Laboratory (Western University)

London, Ontario, Canada

Site Status RECRUITING

Countries

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Canada

Central Contacts

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Crystal Lee, BSc

Role: CONTACT

519 6612111

Arash Bandegan, PhD

Role: CONTACT

519 6612111

Facility Contacts

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Peter Lemon, PhD

Role: primary

519 6612111 ext. 88139

Other Identifiers

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113281

Identifier Type: -

Identifier Source: org_study_id

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