Assessment of Viral Shedding in Children Previously in Receipt of Multiple Doses of Live Attenuated Influenza Vaccine (LAIV) Compared to Influenza Vaccine-naïve Controls

NCT ID: NCT03104790

Last Updated: 2019-08-28

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE4
• Total Enrollment: 373 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2017-10-02
• Study Completion Date: 2018-03-13

Brief Summary

LAIV shedding studies in children could be an important way to confirm whether impediments to viral replication do indeed explain these observed reductions in vaccine effectiveness(VE), whether prior vaccination has any influence on replication and what future implications (if any) this might have for the UK paediatric LAIV programme. LAIV virus replication in children will be dependent on virological and host factors. The virus factors include replicative fitness of individual strains and the susceptibility to inhibition by other replicating strains (ability to compete). Host factors which may influence this include pre-existing specific immunity as a result of prior infection or previous vaccination (with either LAIV or IIV), and innate immune factors including mucosal immunity. Understanding the relative importance of different factors over two seasons when the strain composition of the A/H1N1pdm09 LAIV virus will change and by comparing previously unvaccinated and highly vaccinated groups (with both LAIV and IIV), can potentially give unique insights into their contribution to the US LAIV observations.

With the change of the A/H1N1pdm09 vaccine strain in 2017/18, demonstrating improved performance (in terms of VE, virus shedding and immunogenicity) and what contribution prior vaccination might make will be key evidence for both the UK, but also the US. Information presented at the ACIP in June 2018 from the 2016/17 and 2017/18 seasons will be key to inform US future decisions around use of LAIV.

This is a parallel group, non randomised study which will enrol at least 400 children. Both written informed consent from parent/ guardian and written assent from the child will be in place prior to any study procedure. The two groups will be defined by previous influenza vaccination history, with around half the children naïve to any influenza vaccination (LAIV or IIV) and half having had at least three doses of LAIV with or without IIV. All will follow the same schedule of vaccination and oral fluid collection at day 0 (by the nurse in the home or at the GP surgery); nasal swab collection (by the parent at home on days 1,3,6); day 21 oral fluid collection (by nurse or parent at home or at GP surgery).

Detailed Description

The United States of America (USA) has a long-standing paediatric influenza vaccination programme, including use of live attenuated influenza vaccine (LAIV). Following evidence of lack effectiveness of LAIV in 2015/16, the USA suspended use in the 2016/17 season. The UK introduced LAIV for children in 2013/14 and has since been closely monitoring programme performance. In 2015/16, the UK - in contrast to the USA - found evidence of significant effectiveness of LAIV against laboratory confirmed influenza in both primary and secondary care including against A/H1N1pdm09. The UK results concord with those from several other geographical settings, although several studies report relatively lower effectiveness of LAIV against A/H1N1pdm09 infection compared to inactivated influenza vaccine (IIV). The reasons for these apparent differences in effectiveness are currently unclear.

The USA has indicated that for the Advisory Committee on Immunisation Practice (ACIP) to rescind their decision to suspend use of LAIV, they will require an understanding of the likely underlying mechanism for the apparent reduction in LAIV A/H1N1pdm09 vaccine effectiveness (VE) measured in observational studies in the USA and then evidence that the problem has been resolved.

Several hypotheses are emerging to explain the apparent reduction in A/H1N1pdm09 quadrivalent LAIV effectiveness in the USA last season and their discordance with findings elsewhere including the UK together with the possible lower effectiveness of LAIV against A/H1N1pdm09 compared to IIV. These include one or more of the following:

1. Center for Disease Control (CDC)/ Department of Defense (DoD) specific finding - related to chance, methodology, programmatic issues

2. Reduced replicative fitness of the current A/H1N1pdm09 strain.

3. Viral interference/competition between A/H1N1pdm09 vaccine strain and other vaccine viruses in multivalent formulation;

4. Prior vaccination with LAIV or IIV resulting in specific immunological interference with H1N1pdm09 vaccine virus replication;

5. Repeat LAIV vaccination resulting in broader, longer term immunological changes affecting all viruses (mimicking adult response);

6. Combinations of the above Based upon in vitro studies, the manufacturer of LAIV (MedImmune) have stated that reduced replicative fitness of the A/H1N1pdm09 strain is likely to be the important root cause. However, this factor alone cannot explain the difference in effectiveness observed between the USA and sites elsewhere including the UK. This suggests that there is an important additional factor(s) involved. The US programme has been running for many more years than the UK - and in addition children 6 months to 24 months of age are offered IIV, unlike the UK. These prior vaccine exposures are potential contributory factors.

LAIV shedding studies in children could be an important way to confirm whether impediments to viral replication do indeed explain these observed reductions in VE, whether prior vaccination has any influence on replication and what future implications (if any) this might have for the UK paediatric LAIV programme. LAIV virus replication in children will be dependent on virological and host factors. The virus factors include replicative fitness of individual strains and the susceptibility to inhibition by other replicating strains (ability to compete). Host factors which may influence this include pre-existing specific immunity as a result of prior infection or previous vaccination (with either LAIV or IIV), and innate immune factors including mucosal immunity. Understanding the relative importance of different factors over two seasons when the strain composition of the A/H1N1pdm09 LAIV virus will change and by comparing previously unvaccinated and highly vaccinated groups (with both LAIV and IIV), can potentially give unique insights into their contribution to the US LAIV observations.

With the change of the A/H1N1pdm09 vaccine strain in 2017/18, demonstrating improved performance (in terms of VE, virus shedding and immunogenicity) and what contribution prior vaccination might make will be key evidence for both the UK, but also the US. Information presented at the ACIP in June 2018 from the 2016/17 and 2017/18 seasons will be key to inform US future decisions around use of LAIV.

This is a parallel group, non randomised study which will enrol at least 400 children. Both written informed consent from parent/ guardian and written assent from the child will be in place prior to any study procedure. The two groups will be defined by previous influenza vaccination history, with around half the children naïve to any influenza vaccination (LAIV or IIV) and half having had at least three doses of LAIV with or without IIV. All will follow the same schedule of vaccination and oral fluid collection at day 0 (by the nurse in the home or at the GP surgery); nasal swab collection (by the parent at home on days 1,3,6); day 21 oral fluid collection (by nurse or parent at home or at GP surgery).

Conditions

Influenza Vaccination; Influenza; Vaccination

Study Design

• Allocation Method: NON_RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: PREVENTION
• Blinding Strategy: NONE

Study Groups

naive

Children who have never received any influenza vaccine (The two groups are defined by immunisation history, all receive the same intervention in the study)

Group Type: EXPERIMENTAL

Fluenz Tetra (The two groups are defined by immunisation history, all receive the same intervention in the study)

Intervention Type: BIOLOGICAL

live attenuated influenza vaccine (LAIV)

prior vaccinees

Children who have received at least two doses of Fluenz Tetra previously (The two groups are defined by immunisation history, all receive the same intervention in the study)

Group Type: EXPERIMENTAL

Fluenz Tetra (The two groups are defined by immunisation history, all receive the same intervention in the study)

Intervention Type: BIOLOGICAL

live attenuated influenza vaccine (LAIV)

Interventions

Fluenz Tetra (The two groups are defined by immunisation history, all receive the same intervention in the study)

live attenuated influenza vaccine (LAIV)

Intervention Type: BIOLOGICAL

Eligibility Criteria

Inclusion Criteria

-

• Children age 6 to 13 years of age on enrolment and with either:

• Prior LAIV vaccination in at least 2 out of the 3 previous years
• Have never received LAIV or IIV in previous years
• Children eligible to receive LAIV in accordance with Green Book advice [https://www.gov.uk/government/organisations/public-health-england/series/immunisation-against-infectious-disease-the-green-book]
• Written informed consent given by parent/ guardian and assent from child (both must be in place to proceed)

Exclusion Criteria

-

Children may not be included in the study if any of the following apply:

1. Admission to Paediatric ntensice care unit (PICU) for invasive ventilation due to a respiratory illness in the preceding 2 years.

2. Contraindications to LAIV (notwithstanding allergy to egg protein), which include:

1. Hypersensitivity to the active ingredients, gelatin or gentamicin (a possible trace residue)

2. Previous systemic allergic reaction to LAIV

3. Previous allergic reaction to an influenza vaccine (not LAIV) is a relative contra-indication, which must be discussed with the CI to confirm patient suitability

4. Children/adolescents who are clinically immunodeficient due to conditions or immunosuppressive therapy such as: acute and chronic leukaemias; lymphoma; symptomatic HIV infection; cellular immune deficiencies; and high-dose corticosteroids*.

*High-dose steroids is defined as a treatment course for at least one month, equivalent to a dose greater than 20mg prednisolone per day (any age), or for children under 20kg, a dose greater than 1mg/kg/day.

NB: LAIV is not contraindicated for use in individuals with asymptomatic HIV infection; or individuals who are receiving topical/inhaled/low-dose oral systemic corticosteroids or those receiving corticosteroids as replacement therapy, e.g. for adrenal insufficiency.

5. Children / adolescents younger than 18 years of age receiving salicylate therapy because of the association of Reye's syndrome with salicylates and wild-type influenza infection.

6. Pregnancy

3. Contraindications to vaccination on that occasion, e.g. due to child being acutely unwell:

1. Febrile ≥38.0oC in last 72 hours

2. **Acute wheeze in last 72 hours requiring treatment beyond that normally prescribed for regular use by the child's treating healthcare professional

3. **Recent admission to hospital in last 2 weeks for acute asthma

4. **Current oral steroid for asthma exacerbation or course completed within last 2 weeks

5. Any other significant condition or circumstance which, in the opinion of the investigator, may either put the participant at risk because of participation in the study, or may influence the result of the study, or the participant's ability to participate in the study.

• Items 3b-3d are relative contra-indications: Many children with "difficult-to-control" symptoms fail to meet these criteria on a routine basis. Where these are present, the CI is able to authorise participation on a case-by-case basis, after assessing the child at the time of enrolment.

Administration of another live vaccine (e.g. MMR) within the previous 4 weeks is no longer a contra-indication to LAIV administration, according to updated UK Department of Health guidelines.

NB: See Summary of Product Characteristics for full details of contra-indications to LAIV.

• Minimum Eligible Age: 6 Years
• Maximum Eligible Age: 13 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Public Health England

OTHER_GOV

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Elizabeth Coates, PhD

Role: STUDY_CHAIR

Public Health England

Locations

Gloucestershire primary care

Gloucester, Gloucestershire, United Kingdom

Hertfordshire primary care

Hertford, Hertfordshire, United Kingdom

Imperial Healthcare NHS Trust

London, , United Kingdom

Countries

United Kingdom

References

Jackson D, Pitcher M, Hudson C, Andrews N, Southern J, Ellis J, Hoschler K, Pebody R, Turner PJ, Miller E, Zambon M. Viral Shedding in Recipients of Live Attenuated Influenza Vaccine in the 2016-2017 and 2017-2018 Influenza Seasons in the United Kingdom. Clin Infect Dis. 2020 Jun 10;70(12):2505-2513. doi: 10.1093/cid/ciz719.

Reference Type: DERIVED

PMID: 31642899 (View on PubMed)

Other Identifiers

Flushed

Identifier Type: -

Identifier Source: org_study_id