Influenza Vaccine Challenge Study in Healthy Subjects

NCT ID: NCT01226758

Last Updated: 2012-11-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

32 participants

Study Classification

INTERVENTIONAL

Study Start Date

2010-06-30

Study Completion Date

2010-12-31

Brief Summary

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The purpose of this research is to study the safety, tolerability and effectiveness of the investigational influenza vaccine in healthy volunteers infected with an attenuated influenza A virus.

Detailed Description

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The investigational influenza vaccine (FLU-v) contains multiple highly conserved T cell epitopes that are present on most influenza viruses, which have been identified as reactive in different human leukocyte antigen (HLA) populations; thus making it unlikely that anybody in the vaccinated population would be unable to mount an immune response to at least one of the epitopes contained in the vaccine.

In this study up to 44 will be vaccinated with the FLU-v experimental vaccine or a placebo in a 1:1 ratio. Volunteers will attend a screening visit, a vaccination visit, a 10-11 day overnight stay in a quarantine facility, and a follow-up visit to the P1 clinic.

Three weeks after being vaccinated with FLU-v or placebo, 30 volunteers will be taken to a Quarantine Unit to be exposed to the attenuated H3N2 study virus and then monitored by study physicians and nurses for a 10-11 day period. The other 14 volunteers will be held in reserve as back-ups. If fewer than 30 of the volunteers who travel to the Quarantine Unit are eligible to be exposed to the study virus, then volunteers who have been kept in reserve will be used.

Following discharge from the Quarantine Unit, volunteers will attend one study follow-up visit, 28 days after exposure to the study virus. Assessments will take place and samples will be taken from volunteers.

Conditions

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Influenza

Keywords

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Safety Tolerability Efficacy Influenza Flu Virus Vaccine

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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FLU-v with adjuvant

Group Type EXPERIMENTAL

Influenza vaccine (FLU-v)

Intervention Type BIOLOGICAL

FLU-v (sterile lyophilised mixture of polypeptide T-cell epitope sequences) and adjuvant. Administered by single subcutaneous injection.

Placebo

Adjuvant only placebo

Group Type PLACEBO_COMPARATOR

Placebo (adjuvant only)

Intervention Type BIOLOGICAL

Single subcutaneous injection of adjuvant

Interventions

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Influenza vaccine (FLU-v)

FLU-v (sterile lyophilised mixture of polypeptide T-cell epitope sequences) and adjuvant. Administered by single subcutaneous injection.

Intervention Type BIOLOGICAL

Placebo (adjuvant only)

Single subcutaneous injection of adjuvant

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* General good health determined by a screening evaluation ≤120 days prior to IMP administration and on the day of admittance to quarantine
* Using methods of contraception, (e.g. spermicidal gel plus condom) for the entire duration of the study, up to the Study Completion visit, and refrain from fathering a child in the three months following study drug administration.
* Negative HIV, hepatitis B and C antibody screens
* Negative class A drugs, alcohol and nicotine screen
* Seronegative (≤10 HAI) for challenge virus
* Have not been vaccinated for influenza virus since 2006 (as determined in the medical history) or had a known influenza-like illness in the current season, defined as in the last 12 months

Exclusion Criteria

* Presence of any significant acute or chronic, uncontrolled medical or psychiatric illness, including but not exclusive to the conditions listed in Appendix 2, that in the view of the Investigator is associated with increased risk of complications of respiratory viral illness
* Abnormal pulmonary function as evidenced by clinically significant abnormalities in spirometry
* Presence of household member or close contact who is: less than 3 years of age; known immunodeficient; receiving immunosuppressants; undergoing/soon to undergo chemotherapy; diagnosed with emphysema or COPD; is elderly residing in a nursing home; severe lung disease or medical condition; received a transplant (bone marrow or solid organ)
* History of asthma, COPD, pulmonary hypertension, reactive airway disease, or any chronic lung condition of any aetiology
* Any laboratory test or ECG which is abnormal and deemed by the investigator to be clinically significant
* Venous access inadequate for phlebotomy demands
* Regular daily smokers during the 6 months prior to study entry or those who have a significant history of any tobacco use at any time
* Subject is diabetic
* History or evidence of autoimmune disease or known impaired immune responsiveness
* Recent and/or recurrent history of autonomic dysfunction
* Receipt of systemic glucocorticoids, antiviral drugs, immunoglobulins or blood transfusions within 1 month, or any other cytotoxic or immunosuppressive drug within 6 months prior to vaccination. Receipt of any systemic chemotherapy agent at any time
* Presence of any febrile illness or symptoms of upper or lower tract respiratory infection in the 28 days prior to viral inoculation
* Any anatomic or neurological abnormality impairing the gag reflex or associated with an increased risk of aspiration, or history suggestive of such a problem or any abnormality significantly altering the anatomy of the nose or nasopharynx
* Known IgA deficiency, immotile cilia syndrome, or Kartagener's syndrome
* Nasal or sinus surgery within 30 days prior to vaccination
* Significant history of seasonal hay fever or a seasonal allergic rhinitis (SAR), or perennial allergic rhinitis (PAR), or chronic nasal or sinus condition
* Acute and/or chronic use of any medication or other product for symptoms of rhinitis or nasal congestion or for any chronic nasopharyngeal complaint, or chronic use of any intranasal medication for any indication
* Use of any prescription drugs, herbal supplements, within 4 weeks prior to vaccine administration
* Receipt of any investigational drug within 3 months, or prior participation in a clinical trial of any influenza vaccine, medication or experimental Influenza viral challenge delivered directly to the respiratory tract within 1 year
* Previous exposure to the IMP or similar substance
* History of multiple and recurring allergies and/or adverse reaction to any components of the IMP and challenge virus preparation
* History of allergy or intolerance to the following drugs: oseltamivir or zanamivir.
* Allergic to gentamicin
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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PepTcell Limited

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Anthony Gilbert, MBBCh, MICR

Role: PRINCIPAL_INVESTIGATOR

Retroscreen Virology Limited

Locations

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Retroscreen Virology Limited

London, , United Kingdom

Site Status

Countries

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United Kingdom

References

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Pleguezuelos O, Robinson S, Fernandez A, Stoloff GA, Caparros-Wanderley W. Meta-Analysis and Potential Role of Preexisting Heterosubtypic Cellular Immunity Based on Variations in Disease Severity Outcomes for Influenza Live Viral Challenges in Humans. Clin Vaccine Immunol. 2015 Aug;22(8):949-56. doi: 10.1128/CVI.00101-15. Epub 2015 Jun 17.

Reference Type DERIVED
PMID: 26084515 (View on PubMed)

Pleguezuelos O, Robinson S, Fernandez A, Stoloff GA, Mann A, Gilbert A, Balaratnam G, Wilkinson T, Lambkin-Williams R, Oxford J, Caparros-Wanderley W. A Synthetic Influenza Virus Vaccine Induces a Cellular Immune Response That Correlates with Reduction in Symptomatology and Virus Shedding in a Randomized Phase Ib Live-Virus Challenge in Humans. Clin Vaccine Immunol. 2015 Jul;22(7):828-35. doi: 10.1128/CVI.00098-15. Epub 2015 May 20.

Reference Type DERIVED
PMID: 25994549 (View on PubMed)

Other Identifiers

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FLU-v-002

Identifier Type: -

Identifier Source: org_study_id