Anabolic Effects of Whey and Casein After Strength Training in Young and Elderly

NCT ID: NCT02968888

Last Updated: 2018-04-10

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 43 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2014-08-31
• Study Completion Date: 2017-05-31

Brief Summary

The aim of this study is to investigate the acute anabolic effects of native whey, whey protein concentrate 80 (WPC-80) and milk after a bout of strength training in young and elderly. The investigators hypothesize that native whey will give a greater stimulation of muscle protein synthesis and intracellular anabolic signaling than WPC-80, and that WPC-80 will give a stronger stimulus than milk.

Detailed Description

Increasing or maintaining muscle mass is of great importance for populations ranging from athletes to patients and elderly. Resistance exercise and protein ingestion are two of the most potent stimulators of muscle protein synthesis. Both the physical characteristic of proteins (e.g. different digestion rates of whey and casein) and the amino acid composition, affects the potential of a certain protein to stimulate muscle protein synthesis. Given its superior ability to rapidly increase blood leucine concentrations to high levels, whey is often considered the most potent protein source to stimulate muscle protein synthesis. Native whey protein is produced by filtration of unprocessed milk. Consequently, native whey has different characteristics than WPC-80, which is exposed to heating and acidification. Because of the direct filtration of unprocessed milk, native whey is a more intact protein compared with WPC-80. Of special interest is the higher amounts of the highly anabolic amino acid leucine in native whey.

The higher levels of leucine can be of great interest for elderly individuals as some studies in elderly has shown an anabolic resistance to the effects of protein feeding and strength training. By increasing levels of leucine one might overcome this anabolic resistance in the elderly.

The aim of this double-blinded, randomized, partial cross-over study is to compare the acute fractional protein synthesis and intracellular signaling response to a bout of strength training and intake of 20 grams of protein from either native whey, whey protein concentrate 80 or milk, in young and old individuals. Furthermore, the investigators wil investigate fractional protein breakdown, markers of protein breakdown, amino acid concentrations in blood.

The investigators hypothesize that native whey will induce a greater anabolic response than whey protein concentrate 80, and that whey protein concentrate 80 will give a stronger anabolic response than milk.

Conditions

Healthy; Young; Elderly

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: TRIPLE

Study Groups

Milk

Participants performed a bout of strength training and consumed 20g of milk protein

Group Type: PLACEBO_COMPARATOR

Strength training

Intervention Type: OTHER

Milk 1%

Intervention Type: DIETARY_SUPPLEMENT

Whey protein concentrate 80

Participants performed a bout of strength training and consumed 20g of whey protein concentrate 80

Group Type: EXPERIMENTAL

Strength training

Intervention Type: OTHER

Whey protein concentrate 80

Intervention Type: DIETARY_SUPPLEMENT

Native whey

Participants performed a bout of strength training and consumed 20g of native whey

Group Type: EXPERIMENTAL

Strength training

Intervention Type: OTHER

Native whey

Intervention Type: DIETARY_SUPPLEMENT

Interventions

Strength training

Intervention Type: OTHER

Milk 1%

Intervention Type: DIETARY_SUPPLEMENT

Whey protein concentrate 80

Intervention Type: DIETARY_SUPPLEMENT

Native whey

Intervention Type: DIETARY_SUPPLEMENT

Eligibility Criteria

Inclusion Criteria

• Healthy in the sense that they can conduct training and testing
• Able to understand Norwegian language written and oral
• Between 18 and 45, or above 70 years of age

Exclusion Criteria

• Diseases or injuries contraindicating participation
• Use of dietary supplements (e.g. proteins, vitamins and creatine)
• Lactose intolerance
• Allergy to milk
• Allergy towards local anesthetics (xylocain)

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Tine

INDUSTRY

Sponsor Role: collaborator

The Research Council of Norway

OTHER

Sponsor Role: collaborator

Arkansas Children's Hospital Research Institute

OTHER

Sponsor Role: collaborator

Norwegian School of Sport Sciences

OTHER

Sponsor Role: lead

Responsible Party

Havard Hamarsland

PhD student

Responsibility Role: PRINCIPAL_INVESTIGATOR

Locations

Norwegian School of Sport Sciences

Oslo, , Norway

Countries

Norway

References

Hamarsland H, Nordengen AL, Nyvik Aas S, Holte K, Garthe I, Paulsen G, Cotter M, Borsheim E, Benestad HB, Raastad T. Native whey protein with high levels of leucine results in similar post-exercise muscular anabolic responses as regular whey protein: a randomized controlled trial. J Int Soc Sports Nutr. 2017 Nov 21;14:43. doi: 10.1186/s12970-017-0202-y. eCollection 2017.

Reference Type: DERIVED

PMID: 29200982 (View on PubMed)

Other Identifiers

Tine acute

Identifier Type: -

Identifier Source: org_study_id