MiRNAs Evaluating Lymphocyte Proliferation and Apoptosis as Well as Prognosis of Sepsis

NCT ID: NCT02756559

Last Updated: 2016-05-02

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 100 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2014-01-31
• Study Completion Date: 2017-12-31

Brief Summary

Sepsis is a systemic inflammatory reaction caused by infection, trauma, etc. In the process of sepsis, the apoptosis of lymphocyte serves as a vital factor of immune dysfunction. Micro-RNAs (miRNAs) are endogenous small non-coding RNA molecules. Our previous studies have showed the diagnostic and prognostic value of a series circulant miRNAs in human serum in sepsis. The present research further study the regulatory mechanism of those miRNAs in lymphocyte in sepsis. Our research is based on the clinical value of miR-223, miR-15a, miR-16. The investigators discuss the potential role of miR-223, miR-15a, miR-16 in regulating the proliferation and apoptosis of lymphocyte in sepsis and aim to find new targets for sepsis treatment.

Detailed Description

Blood samples were collected from the Chinese People's Liberation Army General Hospital. The investigators collected samples in emergency intensive care unit (EICU), surgical intensive care unit (SICU) and respiratory intensive care unit (RICU) between July 2014 to March 2015. Septic patients were divided into sepsis, severe sepsis and septic shock according to the diagnostic criteria. Peripheral white blood cells were sorted into monocytes, lymphocytes and neutrophils by using flow cytometry (Flow Cytometry, FCM). Jurkat T cell lines were purchased from Chinese Academy of Medical Sciences Institute of basic medical sciences. miRNAs agomirs and antagonists were used to establish miR-223, miR-15a, miR-16 overexpression and knock-out transient transfection cell lines. Western blot was used to detect difference expression of protein expression.

Conditions

T Lymphocyte Disorder

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: PROSPECTIVE

Study Groups

sepsis, severe sepsis and septic shock

Septic patients were divided into sepsis, severe sepsis and septic shock

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

• Diagnosis of Sepsis
• Agree with this study

Exclusion Criteria

• Age <18 years
• Died within 24 hours
• Combined with HIV infection
• Agranulocytosis

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Lixin Xie

OTHER

Sponsor Role: lead

Responsible Party

Lixin Xie

Clinical Professor

Responsibility Role: SPONSOR_INVESTIGATOR

Principal Investigators

Lixin Xie, Clinical Professor

Role: STUDY_CHAIR

Chinese PLA General Hospital

Locations

Chinese People's Liberation Army General Hospital

Beijing, Beijing Municipality, China

Site Status: RECRUITING

Countries

China

Central Contacts

Dan Liu, Principal Investigator

Role: CONTACT

liudan_nkdx@163.com

+86 13920701949

Facility Contacts

Dan Liu, Principal Investigator

Role: primary

liudan_nkdx@163.com

+86 13920701949

Other Identifiers

S2015-068-01

Identifier Type: -

Identifier Source: org_study_id