Validation of DNA Methylation Biomarkers for Oral Cancer Detection

NCT ID: NCT02648789

Last Updated: 2018-08-09

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

46 participants

Study Classification

OBSERVATIONAL

Study Start Date

2015-05-31

Study Completion Date

2017-05-31

Brief Summary

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The purpose of this study is to investigate the correlation between DNA methylation and the treatment and recurrence of oral cancer.

Detailed Description

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In this study, investigators plan to recruit 40\~50 patients who had, or currently has oral cancer. The study procedures are as below:

* After the Informed Consent Form is signed, swab from the lesion site and normal site to obtain oral epithelial cells.
* Collect tissue from the tumor site and the non-cancerous matched site from the surgery or the tissue bank.
* Collect oral swab from the lesion site and the normal site when patients return to the clinic for a regular follow-up.

Specimens will be collected for DNA methylation testing, and the results will be compared with patient's clinical diagnosis. It is expected that the relationship between DNA methylation, oral cancer, and the recurrence of oral cancer will be found.

Conditions

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Oral Cancer Recurrence

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Eligibility Criteria

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Inclusion Criteria

* Age between 20 and 75 years old
* Had or currently has cancer of the oral cavity
* Agree to sign the Informed Consent Form (ICF) and comply with follow-up procedures

Exclusion Criteria

* Unwilling to sign the Informed Consent Form (ICF)
* Has an autoimmune disease of the oral cavity
Minimum Eligible Age

20 Years

Maximum Eligible Age

75 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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iStat Biomedical CO., Ltd.

INDUSTRY

Sponsor Role collaborator

Taipei Veterans General Hospital, Taiwan

OTHER_GOV

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Cheng-Chieh Yang, DDS., Ph.D.

Role: PRINCIPAL_INVESTIGATOR

Department of Stomatology, Taipei Veterans General Hospital

Shou-Yen Kao, DDS., M.D.S.

Role: PRINCIPAL_INVESTIGATOR

Department of Stomatology, Taipei Veterans General Hospital

Locations

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Taipei Veterans General Hospital

Taipei, , Taiwan

Site Status

Countries

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Taiwan

References

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Wang SS, Smiraglia DJ, Wu YZ, Ghosh S, Rader JS, Cho KR, Bonfiglio TA, Nayar R, Plass C, Sherman ME. Identification of novel methylation markers in cervical cancer using restriction landmark genomic scanning. Cancer Res. 2008 Apr 1;68(7):2489-97. doi: 10.1158/0008-5472.CAN-07-3194.

Reference Type BACKGROUND
PMID: 18381458 (View on PubMed)

Sova P, Feng Q, Geiss G, Wood T, Strauss R, Rudolf V, Lieber A, Kiviat N. Discovery of novel methylation biomarkers in cervical carcinoma by global demethylation and microarray analysis. Cancer Epidemiol Biomarkers Prev. 2006 Jan;15(1):114-23. doi: 10.1158/1055-9965.EPI-05-0323.

Reference Type BACKGROUND
PMID: 16434596 (View on PubMed)

Lai HC, Lin YW, Chang CC, Wang HC, Chu TW, Yu MH, Chu TY. Hypermethylation of two consecutive tumor suppressor genes, BLU and RASSF1A, located at 3p21.3 in cervical neoplasias. Gynecol Oncol. 2007 Mar;104(3):629-35. doi: 10.1016/j.ygyno.2006.10.003. Epub 2006 Nov 13.

Reference Type BACKGROUND
PMID: 17097722 (View on PubMed)

Towle R, Garnis C. Methylation-mediated molecular dysregulation in clinical oral malignancy. J Oncol. 2012;2012:170172. doi: 10.1155/2012/170172. Epub 2012 May 7.

Reference Type BACKGROUND
PMID: 22645611 (View on PubMed)

Garcia MP, Garcia-Garcia A. Epigenome and DNA methylation in oral squamous cell carcinoma. Methods Mol Biol. 2012;863:207-19. doi: 10.1007/978-1-61779-612-8_12.

Reference Type BACKGROUND
PMID: 22359295 (View on PubMed)

Nagata S, Hamada T, Yamada N, Yokoyama S, Kitamoto S, Kanmura Y, Nomura M, Kamikawa Y, Yonezawa S, Sugihara K. Aberrant DNA methylation of tumor-related genes in oral rinse: a noninvasive method for detection of oral squamous cell carcinoma. Cancer. 2012 Sep 1;118(17):4298-308. doi: 10.1002/cncr.27417. Epub 2012 Jan 17.

Reference Type BACKGROUND
PMID: 22252571 (View on PubMed)

Fakhry C, Gillison ML. Clinical implications of human papillomavirus in head and neck cancers. J Clin Oncol. 2006 Jun 10;24(17):2606-11. doi: 10.1200/JCO.2006.06.1291.

Reference Type BACKGROUND
PMID: 16763272 (View on PubMed)

Chaturvedi AK, Engels EA, Anderson WF, Gillison ML. Incidence trends for human papillomavirus-related and -unrelated oral squamous cell carcinomas in the United States. J Clin Oncol. 2008 Feb 1;26(4):612-9. doi: 10.1200/JCO.2007.14.1713.

Reference Type BACKGROUND
PMID: 18235120 (View on PubMed)

Huang YK, Peng BY, Wu CY, Su CT, Wang HC, Lai HC. DNA methylation of PAX1 as a biomarker for oral squamous cell carcinoma. Clin Oral Investig. 2014 Apr;18(3):801-8. doi: 10.1007/s00784-013-1048-6. Epub 2013 Aug 2.

Reference Type BACKGROUND
PMID: 23907469 (View on PubMed)

Other Identifiers

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2015-06-009AC

Identifier Type: -

Identifier Source: org_study_id

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