Biomarkers in Tissue Samples From Patients Who Have Undergone Neck Dissection for Oral Cancer

NCT ID: NCT00899704

Last Updated: 2019-01-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

WITHDRAWN

Study Classification

OBSERVATIONAL

Study Start Date

2008-03-31

Study Completion Date

2008-03-31

Brief Summary

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RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research study is looking at tissue samples in predicting oral cancer in patients who have undergone neck dissection for oral cancer.

Detailed Description

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OBJECTIVES:

* To test preliminary data from node-positive oral squamous cell carcinoma (OSCC) signatures for their ability to predict lymph-positive primaries using a cohort of OSCC tumors.
* To perform lymph node prediction using sentinel lymph node-biopsied primaries.
* To validate the preliminary data node-positive OSCC signature and test its ability to predict nodal status.

OUTLINE: Patient tissue samples are screened using polymerase chain reaction (PCR) for human papilloma virus-specific primers. Samples are analyzed to identify a nodal-metastasis signature for oral squamous cell carcinoma. Samples also undergo microarray analysis to quantify expression levels for targeted genes. Initial data analysis is performed using Affymetrix® Microarray Suite 5.0 to quantify expression levels for targeted genes.

Conditions

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Head and Neck Cancer

Study Design

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Observational Model Type

CASE_ONLY

Study Time Perspective

RETROSPECTIVE

Study Groups

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Group 1

Patient tissue samples are screened using polymerase chain reaction (PCR) for human papilloma virus-specific primers. Samples are analyzed to identify a nodal-metastasis signature for oral squamous cell carcinoma. Samples also undergo microarray analysis to quantify expression levels for targeted genes. Initial data analysis is performed using Affymetrix® Microarray Suite 5.0 to quantify expression levels for targeted genes.

microarray analysis

Intervention Type GENETIC

allele-specific oligonucleotide real-time quantitative polymerase chain reaction

Intervention Type GENETIC

diagnostic laboratory biomarker analysis

Intervention Type OTHER

Interventions

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microarray analysis

Intervention Type GENETIC

allele-specific oligonucleotide real-time quantitative polymerase chain reaction

Intervention Type GENETIC

diagnostic laboratory biomarker analysis

Intervention Type OTHER

Other Intervention Names

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gene expression profiling ASO RQ-PCR

Eligibility Criteria

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Inclusion Criteria

* Have a primary tumor specimen banked by the American College of Surgeons Oncology Group (ACOSOG) as part of clinical trial ACOSOG-Z0360

\* All tumor specimens are squamous cell carcinomas of the tongue, floor of the mouth, or gingiva
* All patients have undergone neck dissections \* Pathologically confirmed nodal stage (negative or positive)
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

Alliance for Clinical Trials in Oncology

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Barry Ziober, PhD

Role: STUDY_CHAIR

Abramson Cancer Center at Penn Medicine

Locations

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Abramson Cancer Center of the University of Pennsylvania

Philadelphia, Pennsylvania, United States

Site Status

Countries

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United States

Other Identifiers

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ACOSOG-Z3081

Identifier Type: -

Identifier Source: secondary_id

CDR0000589332

Identifier Type: REGISTRY

Identifier Source: secondary_id

ACOSOG-Z3081

Identifier Type: -

Identifier Source: org_study_id

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