Clinical Evaluation of the APTIMA® HPV Assay and Comparison With the HR HC2® Test Using LBC ThinPrep® Specimens

NCT ID: NCT02634190

Last Updated: 2024-02-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

10000 participants

Study Classification

OBSERVATIONAL

Study Start Date

2009-06-30

Study Completion Date

2021-12-31

Brief Summary

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To assess and compare the performance of the HR HPV HC2® test (Qiagen/Digene) and the APTIMA® HPV Assay (Hologic) using LBC Specimens (ThinPrep® Pap Test) for the detection of HPV infection and high-grade CIN lesions in a screening population of women 30 years of age or older in Germany.

Detailed Description

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The study is conducted in the areas of Tuebingen, Freiburg and Saarbruecken in Germany. In total, 10.000 ThinPrep® LBC cervical samples were collected from June 2009 to May 2012. Liquid based cytology (LBC) was performed by a central laboratory in Saarbruecken. Human papilloma virus (HPV) testing with the HR HPV HC2® test and APTIMA® HPV Assay were performed at the Section of Experimental Virology, Institute of Medical Virology, University Clinic of Tuebingen, Germany (UKT).

Within a follow-up phase women who tested positive in any test at baseline will be monitored over a period of 10 years.

Study close out visit: In addition, approximately 5 years after baseline ThinPrep® LBC cervical samples will be collected from a random sample of 4000 study participants who tested triple negative at baseline for determination of the longitudinal negative predictive value (NPV) and HPV related disease after a 5 year period. Women who tested positive in any test will undergo colposcopy.

Conditions

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Human Papilloma Virus Infection

Study Design

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Observational Model Type

COHORT

Study Time Perspective

OTHER

Study Groups

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Triple negative women

Women 30 years of age or older coming for routine cervical screening who tested triple negative at baseline with the interventions: Thinprep® LBC, HR HC2® HPV DNA and APTIMA® HPV Assay

Thinprep® LBC

Intervention Type OTHER

liquid based cytology

APTIMA® HPV Assay

Intervention Type OTHER

in vitro diagnostic test

HR HC2® HPV DNA

Intervention Type OTHER

in vitro diagnostic test

Colposcopy

Intervention Type OTHER

Colposcopy

Women tested positive

Women who tested positive in any of the tests Thinprep® LBC, HR HC2® HPV DNA or APTIMA® HPV Assay will undergo colposcopy and be followed up over a ten year period and subjects who tested positive during the follow up assessment will be followed up over a 5 year period on a yearly basis

Thinprep® LBC

Intervention Type OTHER

liquid based cytology

APTIMA® HPV Assay

Intervention Type OTHER

in vitro diagnostic test

HR HC2® HPV DNA

Intervention Type OTHER

in vitro diagnostic test

Colposcopy

Intervention Type OTHER

Colposcopy

Interventions

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Thinprep® LBC

liquid based cytology

Intervention Type OTHER

APTIMA® HPV Assay

in vitro diagnostic test

Intervention Type OTHER

HR HC2® HPV DNA

in vitro diagnostic test

Intervention Type OTHER

Colposcopy

Colposcopy

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Women aged 30 - 60 years
* Women attending gynaecological practices for routine screening
* Women who gave informed consent to participation in the study

Exclusion Criteria

* Women with hysterectomy or known destructive therapy to the cervix
* Women who are pregnant
* Women with an abnormal cytology result during the previous 6 months
* Women with known HIV infection or history of transplants
* Women vaccinated against HPV
* Women participating in another research protocol
Minimum Eligible Age

30 Years

Maximum Eligible Age

60 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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University Hospital Tuebingen

OTHER

Sponsor Role collaborator

Hologic Deutschland GmbH

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Thomas Iftner, Prof.

Role: PRINCIPAL_INVESTIGATOR

University Hospital Tuebingen

References

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Iftner T, Becker S, Neis KJ, Castanon A, Iftner A, Holz B, Staebler A, Henes M, Rall K, Haedicke J, von Weyhern CH, Clad A, Brucker S, Sasieni P. Head-to-Head Comparison of the RNA-Based Aptima Human Papillomavirus (HPV) Assay and the DNA-Based Hybrid Capture 2 HPV Test in a Routine Screening Population of Women Aged 30 to 60 Years in Germany. J Clin Microbiol. 2015 Aug;53(8):2509-16. doi: 10.1128/JCM.01013-15. Epub 2015 May 27.

Reference Type RESULT
PMID: 26019212 (View on PubMed)

Iftner T, Neis KJ, Castanon A, Landy R, Holz B, Woll-Herrmann A, Iftner A, Staebler A, Wallwiener D, Hann von Weyhern C, Neis F, Haedicke-Jarboui J, Martus P, Brucker S, Henes M, Sasieni P. Longitudinal Clinical Performance of the RNA-Based Aptima Human Papillomavirus (AHPV) Assay in Comparison to the DNA-Based Hybrid Capture 2 HPV Test in Two Consecutive Screening Rounds with a 6-Year Interval in Germany. J Clin Microbiol. 2019 Jan 2;57(1):e01177-18. doi: 10.1128/JCM.01177-18. Print 2019 Jan.

Reference Type RESULT
PMID: 30355760 (View on PubMed)

Other Identifiers

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Aptima Study

Identifier Type: -

Identifier Source: org_study_id

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