Prevalence of Human Papillomavirus (HPV) in a Healthy Population: A Feasibility Study of Oropharyngeal Cancer Screening

NCT ID: NCT07033091

Last Updated: 2025-06-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

20000 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-06-01

Study Completion Date

2031-03-30

Brief Summary

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This study defines the prevalence of oral infection with high-risk human papillomaviruses (hrHPV) in individuals over 40 years of age in the Czech Republic. It also establishes an effective methodology for oropharyngeal carcinoma screening and optimization of screening and testing procedures.

Detailed Description

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One of the primary objectives of this study is to clarify the prevalence of oral infection with high-risk human papillomaviruses (hrHPV) in individuals over 40 years of age in the Czech Republic. This research will enable a more precise estimation of the population at risk for oropharyngeal carcinoma, which is essential for the development of targeted preventive and diagnostic strategies.

Another key objective is to establish an effective methodology for oropharyngeal carcinoma screening and to optimize the associated testing procedures. To achieve these goals, participants will be recruited into two predefined cohorts. The first cohort will consist of healthy individuals recruited through cooperating dental and outpatient clinics. The second cohort will involve potential participants identified from available databases (e.g., clinical trial databases, commercial databases, health insurance records), who will be invited to participate via mailed self-collection kits for gargle sampling.

For participants testing positive for oral hrHPV, venous blood samples will be collected at regular intervals to assess the presence of circulating hrHPV DNA. If hrHPV is detected in the oropharynx, participants will be offered self-collection HPV testing of the urogenital tract (cervicovaginal swabs for women or external genital swabs for men).

In both cohorts, the study will evaluate the prevalence of oral (and, if applicable, genital) hrHPV infection, articipation rates, and the return rates of valid samples for hrHPV testing. Additionally, the study will monitor hrHPV persistence and plasma hrHPV DNA in individuals who test positive.

We anticipate that the results of this study will contribute to earlier and more effective diagnosis of oropharyngeal cancer, with potential for significant public health impact by reducing the burden of this disease. For individual participants, involvement in the study may provide an opportunity for clinical follow-up in the event of hrHPV positivity, supporting earlier detection of oropharyngeal carcinoma.

Conditions

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Oropharynx Cancer

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

SCREENING

Blinding Strategy

NONE

Study Groups

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Arm A

Participants over 40 years attending cooperating dental clinics, who agree to the study, complete questionnaire and provide self-sampled gargling sample. These will be tested by CE-IVD-marked HPV diagnostic test. This arm will include approx. 10000 participants.

Group Type EXPERIMENTAL

Self-sampling by gargling kit at dental clinic

Intervention Type DIAGNOSTIC_TEST

Participants will perform a self-sampling at the dental clinic using gargling kit

Questionnaire

Intervention Type OTHER

Participants will answer questionnaire online/paper at their home or at the dental clinic

Blood sampling

Intervention Type DIAGNOSTIC_TEST

Venous blood will be collected from study participants with oral hrHPV positivity, in which the presence of circulating hrHPV DNA will be determined.

Self-sampling of the urogenital area

Intervention Type DIAGNOSTIC_TEST

In case of a positive examination in the oropharynx, the tested individuals will be offered a self-collection HPV examination in the urogenital area (cervicovaginal swab in women or external genital swab in men).

Arm B

Participants over 40 years selected from available databases receive by mail the study information, questionnaire and self-sampling gargling kit. The kits will be sent back to laboratory and will be tested by CE-IVD-marked HPV diagnostic test. This arm will include approx. 10000 participants.

Group Type EXPERIMENTAL

Questionnaire

Intervention Type OTHER

Participants will answer questionnaire online/paper at their home or at the dental clinic

Self-sampling by gargling kit at home

Intervention Type DIAGNOSTIC_TEST

Participants will perform a self-sampling at home using gargling kit

Blood sampling

Intervention Type DIAGNOSTIC_TEST

Venous blood will be collected from study participants with oral hrHPV positivity, in which the presence of circulating hrHPV DNA will be determined.

Self-sampling of the urogenital area

Intervention Type DIAGNOSTIC_TEST

In case of a positive examination in the oropharynx, the tested individuals will be offered a self-collection HPV examination in the urogenital area (cervicovaginal swab in women or external genital swab in men).

Interventions

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Self-sampling by gargling kit at dental clinic

Participants will perform a self-sampling at the dental clinic using gargling kit

Intervention Type DIAGNOSTIC_TEST

Questionnaire

Participants will answer questionnaire online/paper at their home or at the dental clinic

Intervention Type OTHER

Self-sampling by gargling kit at home

Participants will perform a self-sampling at home using gargling kit

Intervention Type DIAGNOSTIC_TEST

Blood sampling

Venous blood will be collected from study participants with oral hrHPV positivity, in which the presence of circulating hrHPV DNA will be determined.

Intervention Type DIAGNOSTIC_TEST

Self-sampling of the urogenital area

In case of a positive examination in the oropharynx, the tested individuals will be offered a self-collection HPV examination in the urogenital area (cervicovaginal swab in women or external genital swab in men).

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Participants aged 40 years or older at the time of enrollment.
* Individuals who are capable of rinsing the oral cavity by gargling independently.
* Signed informed consent for study participation, molecular-genetic testing, and personal data processing.

Exclusion Criteria

\- Previously diagnosed head and neck cancer.
Minimum Eligible Age

40 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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National Institute for Cancer Research, Czech Republic

OTHER

Sponsor Role collaborator

The Institute of Molecular and Translational Medicine, Czech Republic

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Marian Hajduch, Ph.D.

Role: STUDY_DIRECTOR

Faculty of Medicine and Dentistry, Palacky University, Olomouc

Locations

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Klinika zubního lékařství Fakultní nemocnice Olomouc

Olomouc, , Czechia

Site Status RECRUITING

Countries

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Czechia

Central Contacts

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Vladimira Koudelakova, Ph.D.

Role: CONTACT

+420585632089

Facility Contacts

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Iva Voborná, M.D.

Role: primary

+420585859229

Other Identifiers

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10000

Identifier Type: -

Identifier Source: org_study_id

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