Saliva Testing for High-Risk Human Papillomavirus Infection Oral Cavity and Pharynx Cancer

NCT ID: NCT06362421

Last Updated: 2025-07-31

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

WITHDRAWN

Study Classification

OBSERVATIONAL

Study Start Date

2025-08-31

Study Completion Date

2026-08-31

Brief Summary

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The purpose of this research study is to determine if saliva and oral swab samples can be used to detect human papillomavirus in patients with cancer. In this study, the methods required to detect human papillomavirus will be developed and tested in samples collected from patients with oropharyngeal squamous cell carcinoma and compared to samples collected from participants without cancer.

Detailed Description

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To validate the detection of human papillomavirus 16 and 18 and reference gene ACTB in saliva and buccal swab samples obtained from patients with oropharyngeal squamous cell carcinoma (OPSCC) using the LAMP/CRISPR/electrochemical workflow.

Conditions

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Oropharyngeal Squamous Cell Carcinoma Human Papillomavirus Infection

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Treatment naive patients with HPV+ OPSCC

Participants will receive a pre-study visit and pre-radiation dental evaluation.

Pre-Radiation Dental Evaluation/Sample Collection

Intervention Type GENETIC

Collection of saliva samples and mouth swabs and pre-cancer dental evaluation by study staff. Unstimulated saliva will be collected then a buccal swab sample will be obtained. Participants will be asked to rinse their mouth with water, wait 10 minutes, then 1-2 mL of unstimulated saliva will be captured into sterile centrifuge tube. Next, a buccal swab sample will be collected using a sterile cotton swab and immediately placed in a transport tube containing buffer (Tris with low EDTA)

Pre-Study Visit

Intervention Type OTHER

Collection of vital signs, medical history and current medications

Control Group - Healthy subjects without cancer

Participants will receive a pre-study visit and oral medicine consultation visit.

Pre-Study Visit

Intervention Type OTHER

Collection of vital signs, medical history and current medications

Oral Medicine Consultation Visit/Sample Collection

Intervention Type GENETIC

Collection of saliva samples and mouth swab, complete oral medicine consultation by study staff. Unstimulated saliva will be collected then a buccal swab sample will be obtained. Participants will be asked to rinse their mouth with water, wait 10 minutes, then 1-2 mL of unstimulated saliva will be captured into sterile centrifuge tube. Next, a buccal swab sample will be collected using a sterile cotton swab and immediately placed in a transport tube containing buffer (Tris with low EDTA)

Interventions

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Pre-Radiation Dental Evaluation/Sample Collection

Collection of saliva samples and mouth swabs and pre-cancer dental evaluation by study staff. Unstimulated saliva will be collected then a buccal swab sample will be obtained. Participants will be asked to rinse their mouth with water, wait 10 minutes, then 1-2 mL of unstimulated saliva will be captured into sterile centrifuge tube. Next, a buccal swab sample will be collected using a sterile cotton swab and immediately placed in a transport tube containing buffer (Tris with low EDTA)

Intervention Type GENETIC

Pre-Study Visit

Collection of vital signs, medical history and current medications

Intervention Type OTHER

Oral Medicine Consultation Visit/Sample Collection

Collection of saliva samples and mouth swab, complete oral medicine consultation by study staff. Unstimulated saliva will be collected then a buccal swab sample will be obtained. Participants will be asked to rinse their mouth with water, wait 10 minutes, then 1-2 mL of unstimulated saliva will be captured into sterile centrifuge tube. Next, a buccal swab sample will be collected using a sterile cotton swab and immediately placed in a transport tube containing buffer (Tris with low EDTA)

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

* Patients must have histologically confirmed oropharyngeal squamous cell carcinoma without prior treatment. A pathology report should be referenced/available.
* Patients with p16 positive oropharyngeal squamous cell carcinoma.
* Age 39 to 59 years.
* Ability to understand and the willingness to sign an IRB-approved informed consent document directly.


* Age 39 to 59 years.
* Ability to understand and the willingness to sign an IRB-approved informed consent document directly.

Exclusion Criteria

* Patients with diagnoses of other cancers.
* Patients with current or previous diagnosis with HPV+ infection status for cervical cancer or other cancers.


* Previous diagnosis of cervical cancer or other cancers.
* Presence of known active oral infections of viral, fungal, or bacterial etiology except for gingivitis, periodontitis, or periapical abscess. Participants with known gingivitis, periodontitis, or periapical abscess are included.
Minimum Eligible Age

39 Years

Maximum Eligible Age

59 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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InnoTech Precision Medicine

UNKNOWN

Sponsor Role collaborator

Wake Forest University Health Sciences

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Other Identifiers

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ONC-HN-2402

Identifier Type: OTHER

Identifier Source: secondary_id

IRB00115786

Identifier Type: -

Identifier Source: org_study_id

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