Clinical Evaluation of the APTIMA® HPV Assay Using the TIGRIS® System

NCT ID: NCT00973362

Last Updated: 2017-01-06

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE3

Total Enrollment

12896 participants

Study Classification

INTERVENTIONAL

Study Start Date

2008-03-31

Study Completion Date

2014-04-30

Brief Summary

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The purpose of this study is to provide data on the performance of the APTIMA HPV Assay using the TIGRIS System in detecting HPV types that may cause cervical cancer.

Detailed Description

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The primary objective of the clinical trial is to evaluate the APTIMA HPV Assay, using the TIGRIS System, in cervical cancer screening programs. This will be achieved by determining the performance of the assay for detecting high-risk human papillomavirus (HPV) types in women with known cervical disease status. The assay will be evaluated in sample populations of women with ASC-US Pap test results ("ASC-US results") and women 30 years of age or older ("30+ years of age") with negative (NILM) cytology results.

Conditions

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Human Papillomavirus Infection

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

SCREENING

Blinding Strategy

NONE

Study Groups

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Adjunct (i.e. Normal Pap)

The Adjunct study will evaluate APTIMA HPV Assay clinical performance for detecting high-risk HPV types in female subjects 30+ years of age with negative (NILM) cytology results from routine Pap testing. This will be accomplished by evaluating the assay performance compared to known cervical disease status at baseline and after a 3-year follow-up period. A comparator FDA-Approved HPV DNA test is reported.

Group Type OTHER

APTIMA HPV Assay

Intervention Type DEVICE

The APTIMA HPV Assay is an in vitro nucleic acid amplification test (NAAT) that qualitatively detects human papillomavirus (HPV) E6/E7 messenger RNA (mRNA) from 14 HPV types (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) that are associated with cervical cancer ("high-risk types"). The assay cannot determine the specific high-risk HPV type(s) present and does not cross-react with 5 HPV types that are considered non-oncogenic (not associated with cervical cancer; types 6, 11, 42, 43, and 44).

FDA-Approved HPV DNA Test

Intervention Type DEVICE

A FDA-Approved HPV DNA Test is used as the comparator assay.

ASC-US

The ASC-US study will evaluate the APTIMA HPV Assay clinical performance for detecting high-risk HPV types in subjects with ASC-US Pap test results from routine Pap testing and known cervical disease status (based on colposcopic biopsy results). A comparator FDA-Approved HPV DNA test is reported. There is no follow-up period.

Group Type OTHER

APTIMA HPV Assay

Intervention Type DEVICE

The APTIMA HPV Assay is an in vitro nucleic acid amplification test (NAAT) that qualitatively detects human papillomavirus (HPV) E6/E7 messenger RNA (mRNA) from 14 HPV types (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) that are associated with cervical cancer ("high-risk types"). The assay cannot determine the specific high-risk HPV type(s) present and does not cross-react with 5 HPV types that are considered non-oncogenic (not associated with cervical cancer; types 6, 11, 42, 43, and 44).

FDA-Approved HPV DNA Test

Intervention Type DEVICE

A FDA-Approved HPV DNA Test is used as the comparator assay.

Interventions

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APTIMA HPV Assay

The APTIMA HPV Assay is an in vitro nucleic acid amplification test (NAAT) that qualitatively detects human papillomavirus (HPV) E6/E7 messenger RNA (mRNA) from 14 HPV types (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) that are associated with cervical cancer ("high-risk types"). The assay cannot determine the specific high-risk HPV type(s) present and does not cross-react with 5 HPV types that are considered non-oncogenic (not associated with cervical cancer; types 6, 11, 42, 43, and 44).

Intervention Type DEVICE

FDA-Approved HPV DNA Test

A FDA-Approved HPV DNA Test is used as the comparator assay.

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* Female 21 years or older (ASC-US study only)
* Female 30 years or older (Adjunct and ASC-US studies)
* Subject attending a participating clinic and undergoing a Pap screening exam during the clinic visit
* Ability to comprehend and sign an IRB-approved Informed Consent Form

Exclusion Criteria

* Diagnosis of cervical disease (cancer or precancerous condition) in the past 12 months
* Abnormal Pap test result in the past 12 months
* Subject under 18 years of age and consenting to the study without the presence of their parent or legal guardian
* Known to be pregnant
* Having an illness or medical condition that could interfere with or affect the conduct, results, and/or completion of the clinical trial
* Having an illness or medical condition that the investigator or physician feels would create an unacceptable risk to the subject if she enrolled into the clinical trial
* Prior vaccination for HPV
Minimum Eligible Age

21 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Gen-Probe, Incorporated

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jennifer L Reid, PhD

Role: STUDY_DIRECTOR

Gen-Probe, Incorporated

Locations

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NEA Clinic Women's Clinic

Jonesboro, Arkansas, United States

Site Status

San Fernando Valley Research

Canoga Park, California, United States

Site Status

Healthcare Partners of Monterey Park

Los Angeles, California, United States

Site Status

Diverse Research Solutions

Oxnard, California, United States

Site Status

REMEK

Pomona, California, United States

Site Status

West Coast OB-Gyn

San Diego, California, United States

Site Status

HealthCare Partners

Torrance, California, United States

Site Status

Penninsula Research Associates

Torrance, California, United States

Site Status

Community Medical Research of South Florida

Miami, Florida, United States

Site Status

Insignia Clinical Research

Tampa, Florida, United States

Site Status

Emory University School of Medicine

Atlanta, Georgia, United States

Site Status

Indiana Univ. School of Medicine Dept of Pathology Wishard Health Sciences

Indianapolis, Indiana, United States

Site Status

Saginaw Valley Medical Research

Saginaw, Michigan, United States

Site Status

PPS Clinical

Chesterfield, Missouri, United States

Site Status

Planned Parenthood of Eastern Arkansas and Oklahoma

Tulsa, Oklahoma, United States

Site Status

Temple University Hospital

Philadelphia, Pennsylvania, United States

Site Status

Adams Patterson OB-GYN

Memphis, Tennessee, United States

Site Status

Countries

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United States

Other Identifiers

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2007HPVASCUS30

Identifier Type: -

Identifier Source: org_study_id

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