Human Muscle Gene Expression Profiles in Young and Old Men

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

Get a concise snapshot of the trial, including recruitment status, study phase, enrollment targets, and key timeline milestones.

Recruitment Status

COMPLETED

Total Enrollment

41 participants

Study Classification

OBSERVATIONAL

Study Start Date

2008-10-31

Study Completion Date

2009-03-31

Brief Summary

Review the sponsor-provided synopsis that highlights what the study is about and why it is being conducted.

To reach the goals of living longer in better medical conditions, many countries reach the same conclusion: new strategies have to be developed to avoid, or at least limit, the effects of age; this requires a better knowledge of the mechanisms of aging. Our project focuses on the loss of muscle mass associated with aging, called sarcopenia. Sarcopenia unavoidably leads to impaired mobility and poor balance, which contributes to loss of functional autonomy and to increased prevalence for severe falls. Skeletal muscle also plays a central role as a reserve for energy and amino acids. Hence, sarcopenia further triggers severe side metabolic effects such as frailty among elderly persons. The precise mechanisms of muscle aging are still mostly unknown, although many theories have been proposed.

The present study aims at better understanding the mechanisms of skeletal muscle loss associated with aging. Using muscle biopsies from young and old subjects, the differential expression profiles of mRNA will be obtained through chips that will evaluate more than 39000 transcripts. On the same samples, proteomic analyses will involve two complementary approaches: (1) bidimensional electrophoresis (2DGE) coupled to mass spectrometry (MALDI-ToF) for dominant proteins; (2) Western-blot (more than 800 antibodies) targeting regulating proteins not detectable using 2DGE. Complementary histological studies (immunohisto-fluorescence, confocal microscopy) will specify the localisation of the major biomarkers in the muscle biopsies.

The results of that research will have applications in the medium term and will lead to nutritional interventions to modulate specific metabolic pathways and improve the quality of life in the elderly.

Related Clinical Trials

Explore similar clinical trials based on study characteristics and research focus.

Biological Aging of Skeletal Muscles in Humans

NCT02675192

HEALTHY

COMPLETED

Biological & Fonctional Signatures for Muscle Failures, Aged People & Personalized Physical Activity

NCT05343286

Elderly

RECRUITING NA

Muscle Ageing Sarcopenia Study Lifecourse (MASS Lifecourse)

NCT04239495

Sarcopenia

UNKNOWN

Structural and Metabolic Determinants of Sarcopenia and the Efficacy of Concentric vs. Eccentric Exercise Training

NCT02142855

Healthy

COMPLETED NA

Diagnosis of Sarcopenia in Older Adults

NCT07011030

Sarcopenia

COMPLETED NA

Detailed Description

Dive into the extended narrative that explains the scientific background, objectives, and procedures in greater depth.

Conditions

See the medical conditions and disease areas that this research is targeting or investigating.

Elderly Sarcopenia

Study Design

Understand how the trial is structured, including allocation methods, masking strategies, primary purpose, and other design elements.

Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

Review each arm or cohort in the study, along with the interventions and objectives associated with them.

Young

Young men 19-25 years old

Skeletal muscle aging

Intervention Type OTHER

W1 - Blood test

* Maximal voluntary contraction of quadriceps
* Hydrostatic weighing

W2 - Maximal metabolic test using a cycloergometer

W3 - Muscular biopsy

Old

old men 70-76 years old, who participate in the PROOF study (NCT 00759304)

Skeletal muscle aging

Intervention Type OTHER

W1 - Blood test

* Maximal voluntary contraction of quadriceps
* Hydrostatic weighing

W2 - Maximal metabolic test using a cycloergometer

W3 - Muscular biopsy

Interventions

Learn about the drugs, procedures, or behavioral strategies being tested and how they are applied within this trial.

Skeletal muscle aging

W1 - Blood test

* Maximal voluntary contraction of quadriceps
* Hydrostatic weighing

W2 - Maximal metabolic test using a cycloergometer

W3 - Muscular biopsy

Intervention Type OTHER

Eligibility Criteria

Check the participation requirements, including inclusion and exclusion rules, age limits, and whether healthy volunteers are accepted.

Inclusion Criteria

* Affiliated or beneficiary of a social security category
* Having signed the inform consent form
* Having signed the genetic consent form

Exclusion Criteria

* Antiaggregant platelet treatment
* Type 2 diabetes
* Body Mass Index \> 25
* Severe renal disease
* CRP \> 10
* Abnormal clotting test
* Allergies to local anesthetics

Minimum Eligible Age

19 Years

Maximum Eligible Age

76 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes