Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

NCT ID: NCT00441714

Last Updated: 2015-11-10

Basic Information

• Recruitment Status: WITHDRAWN
• Clinical Phase: NA
• Study Classification: INTERVENTIONAL
• Study Start Date: 2007-10-31
• Study Completion Date: 2007-12-31

Brief Summary

To adjust our forearm model for ischemia reperfusion injury, in order to used it for assessment of ischemia reperfusion damage in elderly and patients.

To test the effect of ischemic postconditioning on ischemia reperfusion injury in healthy volunteers, using Annexin binding after repetitive handgripping.

Detailed Description

Recently, ischemic postconditioning has been identified as a protective intervention against ischemia-reperfusion injury. In animal studies, the signalling pathway of this (impressive) protective phenomenon is very similar to ischemic preconditioning. It opens a new avenue of post-reperfusion interventions with drugs that have been shown to mimic ischemic preconditioning. Before we can study this phenomenon in our forearm ischemia-reperfusion model, we need additional validating experiments. The purpose of this study proposal is to provide these data.

Conditions

Cardiovascular Disease; Ischemia-reperfusion Injury; Postconditioning

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: DIAGNOSTIC
• Blinding Strategy: NONE

Interventions

repetitive short ischemia and reperfusion of non dominant fore arm after 10 minute ischemic exercise

Intervention Type: PROCEDURE

Eligibility Criteria

Inclusion Criteria

• Age 18-50 years
• No physical limitation to perform ischemic exercise
• Informed consent

Exclusion Criteria

• Diabetes (fasting glucose > 7,0mmol/l, or random glucose >11,0mmol/l)
• hyperlipidemia (random total cholesterol > 6.5 mmol/l)
• Hypertension (supine SBP/DBP > 140/90 mmHg at screening)
• Any cardiovascular disease
• Drug abuse
• Concomitant chronic use of medication
• Administration of radioactivity in research setting during the last 5 years
• Participation to any drug-investigation during the previous month as checked with VIP check according to CRCN standard procedures.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 50 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

Radboud University Medical Center

OTHER

Sponsor Role: lead

Principal Investigators

Gerard Rongen, MD PhD

Role: PRINCIPAL_INVESTIGATOR

RUMCN

Locations

Radboud University Nijmegen Medical Centre

Nijmegen, , Netherlands

Countries

Netherlands

References

Rongen GA, Oyen WJ, Ramakers BP, Riksen NP, Boerman OC, Steinmetz N, Smits P. Annexin A5 scintigraphy of forearm as a novel in vivo model of skeletal muscle preconditioning in humans. Circulation. 2005 Jan 18;111(2):173-8. doi: 10.1161/01.CIR.0000151612.02223.F2. Epub 2004 Dec 27.

Reference Type: BACKGROUND

PMID: 15623546 (View on PubMed)

Other Identifiers

IPost

Identifier Type: -

Identifier Source: org_study_id