Cryopreserved Ovarian Tissue Transplantation in Patients Who Have Survived Sarcomas and Hematological Tumors

NCT ID: NCT06829654

Last Updated: 2025-02-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

80 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-08-12

Study Completion Date

2026-08-12

Brief Summary

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The study aims to test for the presence or absence of primary tumor-specific tumor markers in ovarian tissue. Therefore, there will be a first phase in which pathology-specific markers will be identified in the patients' diagnostic pathological material by histological, immunohistochemical, and molecular analysis; then, tumor markers will be sought in cryopreserved ovarian tissue.

Detailed Description

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Standardized strategies for detecting neoplastic cells in ovarian tissue of patients with prior sarcomas or oncohematologic malignancies are not available. To increase the safety of ovarian tissue transplantation, the use of advanced and highly sensitive molecular approaches could greatly improve the detection of malignant cells in ovarian tissue.

The study aims to test for the presence or absence of primary tumor-specific tumor markers in ovarian tissue. Therefore, there will be a first phase in which pathology-specific markers will be identified in the patients' diagnostic pathological material by histological, immunohistochemical, and molecular analysis; then, tumor markers will be sought in cryopreserved ovarian tissue.

Identifying highly sensitive molecular tests would lead to increased safety of transplantation in patients cured of sarcomas and oncohematologic diseases. Overall, applications of the techniques under study would increase the chances of resolving infertility, which often reduces the quality of life of these patients, by offering them the chance to procreate and have biological children. The hope of motherhood is an important psychological support and can greatly help patients cope with the difficult treatment process while minimizing social/psychological support. In the long journey of treatment to defeat the disease, being able to offer an option to at least combat the fear of losing endocrine and reproductive function can have a profound impact not only on the patients themselves, but on the entire family and non-family environment.

Conditions

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Sarcomas Haematological Malignancies

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

OTHER

Blinding Strategy

NONE

Study Groups

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Patients who request cryopreservation of ovarian tissue

Patients who request cryopreservation of ovarian tissue

Group Type OTHER

Step 1

Intervention Type OTHER

Cryopreservation of ovarian tissue

Step 2

Intervention Type OTHER

Analysis of tumor markers in the primary tumor

Step 3

Intervention Type OTHER

Search for neoplastic cells in cryopreserved ovarian tissue

Patients with already cryopreserved ovarian tissue at the criobank

Patients with already cryopreserved ovarian tissue at the criobank

Group Type OTHER

Step 2

Intervention Type OTHER

Analysis of tumor markers in the primary tumor

Step 3

Intervention Type OTHER

Search for neoplastic cells in cryopreserved ovarian tissue

Patients who request ovarian tissue reimplantation

Patients who request ovarian tissue reimplantation

Group Type OTHER

Step 2

Intervention Type OTHER

Analysis of tumor markers in the primary tumor

Step 3

Intervention Type OTHER

Search for neoplastic cells in cryopreserved ovarian tissue

Step 4

Intervention Type OTHER

Cryopreserved ovarian tissue autotransplantation

Interventions

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Step 1

Cryopreservation of ovarian tissue

Intervention Type OTHER

Step 2

Analysis of tumor markers in the primary tumor

Intervention Type OTHER

Step 3

Search for neoplastic cells in cryopreserved ovarian tissue

Intervention Type OTHER

Step 4

Cryopreserved ovarian tissue autotransplantation

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

For all groups:

* Patients with Ewing (ES) and synovial (SS) sarcoma, acute lymphoblastic leukemia (LLA), acute myeloid leukemia (LMA), chronic myeloid leukemia (LMC) and myelodysplasia (MDS)
* No objection to the procedure of sampling for cryopreservation or reimplantation of ovarian tissue by the patient's reference oncologist (according to PA105)
* Obtaining informed consent.

Group 1:

* Age \>18 and under 38 at the time of cryopreservation
* Request for cryopreservation of ovarian tissue at the Laboratory Cryopreservation Ovarian Tissue and Cell Cultures/Criobank IRCCS AOUBO

Group 2:

* Age \>18 years
* Ovarian tissue already collected and cryopreserved at the Laboratory for Cryopreservation of Ovarian Tissue and Cell Cultures/Criobank IRCCS AOUBO

Group 3

* Age \> 18 years
* Request for reimplantation of cryopreserved ovarian tissue at the Laboratory for Cryopreservation of Ovarian Tissue and Cell Cultures/Criobank IRCCS AOUBO
* Early menopause or menstrual irregularities

Exclusion Criteria

Group 1 and 3

* Contraindications to laparoscopy
* HIV, hepatitis B and C, treponema pallidum and positive PAP tests
* Malignant disease involving the ovary or samples to be reimplanted

Group 2 None.
Minimum Eligible Age

18 Years

Maximum Eligible Age

38 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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IRCCS Azienda Ospedaliero-Universitaria di Bologna

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Renato Seracchioli, MD

Role: PRINCIPAL_INVESTIGATOR

IRCCS Azienda Ospedaliero-Universitaria di Bologna

Locations

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IRCCS Azienda Ospedaliero-Universitaria di Bologna

Bologna, , Italy

Site Status RECRUITING

Countries

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Italy

Central Contacts

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Renato Seracchioli, MD

Role: CONTACT

+390512143944

Rossella Vicenti

Role: CONTACT

Facility Contacts

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Renato Seracchioli, MD

Role: primary

+390512143944

Rossella Vicenti

Role: backup

Other Identifiers

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CRIOTESOVA2024

Identifier Type: -

Identifier Source: org_study_id

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