Comparison of Serum SIRT-1 and MMP-9 Levels of Patients With Bronchiectasis and Healthy Controls

NCT ID: NCT06589492

Last Updated: 2024-09-19

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 60 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2020-11-02
• Study Completion Date: 2022-09-02

Brief Summary

The purpose of this a cross-sectinol study is to measure the levels of MMP-9 and SIRT-1 levels in patients with bronchiectasis and healthy controls. The aim is to compare the serum levels of these molecules between patients and controls.

• Is the MMP-9 level increasing or decreasing?
• Is the SIRT-1 level increasing or decreasing in patients?
• Is the disease related to age and gender? Participants will only have one 5 ml blood sample drawn when they visit the chest illness clinic. Molecule levels will be measured in this blood's serum.

The patient group participants will be scanned once with high-resolution chest CT.

Apart from this, comparisons will be made using the age and gender information of the participants.

Detailed Description

In the overall study population of 60 subjects who applied to the Sivas Cumhuriyet University Faculty of Medicine Research and Practice Hospital Chest Diseases Clinic. The study population was selected regardless of age and gender. Participants selected bronchiectasis patients (n=30) who had symptoms (cough, sputum production) and had their diagnosis confirmed by a chest high-resolution CT scan within the previous year. The study excluded individuals with bronchiectasis not associated with cystic fibrosis and who had a worsening episode (according to the 2017 European Respiratory Society expert consensus criteria) (16) or had taken antibiotics during the previous four weeks. The study enrolled healthy controls (n=30) through advertisements who had acceptable chest X-ray and spirometry results and no lower airway symptoms or serious systemic disorders. The study eliminated individuals who were pregnant or nursing, as well as those with low comprehension. SIRT-1 and MMP-9 levels in the serum of the study group were determined by the ELISA method.

Conditions

Bronchiectasis Adult

Study Design

• Allocation Method: NON_RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: HEALTH_SERVICES_RESEARCH
• Blinding Strategy: DOUBLE

Study Groups

CT scan

Chest high-resolution CT scan will be performed for diagnosis of patients.

Group Type: NO_INTERVENTION

No interventions assigned to this group

Age, gender

Age and gender information of patients will be obtained for comparison.

Group Type: NO_INTERVENTION

No interventions assigned to this group

Measurement of MMP-9 and SIRT-1 levels in blood serum

It involves taking 5ml of blood from participants to measure mmp-9 and SIRT-1 levels in blood serum.

Group Type: EXPERIMENTAL

Measurement of MMP-9 and SIRT-1 levels in blood serum

Intervention Type: DIAGNOSTIC_TEST

Measurement of MMP-9 and SIRT-1 levels in blood serum, Collection of 5 ml Blood samples

Interventions

Measurement of MMP-9 and SIRT-1 levels in blood serum

Measurement of MMP-9 and SIRT-1 levels in blood serum, Collection of 5 ml Blood samples

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

• Bronchiectasis patients who were symptoms (cough, sputum production)
• Individuals whose diagnosis was confirmed by a high-resolution chest CT scan taken within the previous year

Exclusion Criteria

• Individuals with bronchiectasis associated with cystic fibrosis and who had a worsening episode
• individuals who were pregnant or nursing
• those with low comprehension
• taken antibiotics during the previous four weeks

• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Cumhuriyet University

OTHER

Sponsor Role: lead

Responsible Party

Dilara Ulger Ozbek

principal investigator

Responsibility Role: PRINCIPAL_INVESTIGATOR

Locations

Cumhuriyet University

Sivas, Si̇vas Centre, Turkey (Türkiye)

Countries

Turkey (Türkiye)

Other Identifiers

Biochemistry AD

Identifier Type: -

Identifier Source: org_study_id