New Therapeutic Strategy Against Preeclampsia

NCT ID: NCT06464159

Last Updated: 2024-06-18

Basic Information

• Recruitment Status: NOT_YET_RECRUITING
• Total Enrollment: 100 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2024-06-30
• Study Completion Date: 2026-11-30

Brief Summary

Preeclampsia is a hypertensive disorder of pregnancy associated with important maternal and perinatal mortality. It complicates 2 to 5% of pregnancies and causes more than 70 000 maternal deaths each year worldwide. Although symptomatic management has improved there is currently no curative treatment, and only childbirth and delivery of the placenta, usually prematurely, alleviate the mother's symptoms. The management of extremely preterm infants is a major societal challenge in medical, ethical and economic terms.

Placental insufficiency plays a central role in the pathophysiology of preeclampsia. Abnormal placentation during the first trimester leads to placental hypoperfusion, which induces trophoblast dysfunction and the release in maternal circulation of trophoblastic factors leading to the maternal symptoms. Among molecules that participate to the pathophysiology of preeclampsia, one of the most important players is soluble fms-like tyrosine kinase 1 (sFlt-1), which is a soluble form of the vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) receptor. sFlt-1 binds to free VEGF and PlGF in the maternal circulation, thus reducing their bioavailability for their membrane receptors. Targeting the sFlt-1 pathway is one of the most promising strategies for the development of new treatments for preeclampsia. As sFlt-1 results from alternative splicing, its peptide sequence is identical to that of the extracellular part of the membrane receptor. The development of drugs that act specifically on the soluble form and not on the membrane form is therefore particularly complex.

The general objective of this research is to restore the angiogenic balance that maintains the physiological concentrations of free angiogenic factors in order to significantly prolong the pregnancy and diminish the consequences of the great prematurity. The precise objectives of the APHERESE 2 project are:

1. To transpose the proof of concept of the APHERESE1 project to the scale of a real apheresis column

2. To develop an innovative assay technology to determine the global circulating angiogenic balance for each patient

Detailed Description

The aim of this biobank is to setup a collection of maternal plasma and serum from patients with preeclampsia and patients with normal pregnancy. Assays of circulating angiogenic and anti-angiogenic factors (free and total forms) will be carried out on these serum and plasma at the hormonology laboratory in Cochin hospital (Paris, France) These results will make it possible to optimize and validate the development of extracorporeal sFlt-1 purification techniques.

Preeclampsia is a very heterogeneous disease in its clinical presentation, our hypothesis is that this heterogeneity corresponds to different profiles of angiogenic balance disturbance. Not all patients are likely to have the same expected benefit from extracorporeal clearance of sFlt-1. The objective is to assess the overall angiogenic balance on a large number of patients in order to determine which profile best corresponds to the indication for apheresis.

A prospective non-interventional study will be initiated to collect maternal blood samples during normal pregnancies and during pregnancies complicated by preeclampsia (Port-Royal Maternity, Cochin APHP). Blood samples will be collected after collection of written informed consent from 50 patients with preeclampsia and 50 patients without hypertensive disease.

• Between 20WG and 23WG+6D : 10 patients with PE and 10 patients with NP
• Between 24WG and 27WG+6D : 10 patients with PE and 10 patients with NP
• Between 28WG and 31WG+6D : 10 patients with PE and 10 patients with NP
• Between 32WG and 35WG+6D : 10 patients with PE and 10 patients with NP
• Between 36WG and 40WG+6D : 10 patients with PE and 10 patients with NP Inclusion of the patients will be performed through the Cochin maternal/neonatal investigation center.

Conditions

Preeclampsia

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: PROSPECTIVE

Study Groups

Preeclampsia

Pregnancy with preeclampsia

Biological collection

Intervention Type: BIOLOGICAL

A collection of maternal plasma, serum and urine

Pregnancies with intra uterine growth restriction (IUGR)

Pregnancy with intra uterine growth restriction (IUGR)

Biological collection

Intervention Type: BIOLOGICAL

A collection of maternal plasma, serum and urine

Normal pregnancy

Pregnancy without preeclampsia and intra uterine growth restriction (IUGR) and complications

Biological collection

Intervention Type: BIOLOGICAL

A collection of maternal plasma, serum and urine

Interventions

Biological collection

A collection of maternal plasma, serum and urine

Intervention Type: BIOLOGICAL

Eligibility Criteria

Inclusion Criteria

• Age from 18 to 50 years old
• Singleton pregnancies between 20 and 41 weeks of gestation
• Preeclampsia / normal pregnancy

Exclusion Criteria

• Age < 18 years old
• Infectious disease: HIV, HBV or HCV
• Multiple pregnancies
• refusal to participate in the protocol
• Lack of social security cover

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 50 Years
• Eligible Sex: FEMALE
• Accepts Healthy Volunteers: No

Sponsors

Assistance Publique - Hôpitaux de Paris

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Vassilis TSASARIS, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Assistance Publique - Hôpitaux de Paris

Edouard LECARPENTIER, MD, PhD

Role: STUDY_CHAIR

Institut National de la Santé Et de la Recherche Médicale, France

Jean GUIBOURDENCHE, MD, PhD

Role: STUDY_DIRECTOR

Assistance Publique - Hôpitaux de Paris

Locations

Maternité Port-Royal

Paris, , France

Countries

France

Central Contacts

Vassilis TSASARIS, MD, PhD

Role: CONTACT

vassilis.tsasaris@aphp.fr

+33 158413871

Charly LARRIEU

Role: CONTACT

charly.larrieu@aphp.fr

+33 1 58 41 34 78

Other Identifiers

2023-A01545-40

Identifier Type: OTHER

Identifier Source: secondary_id

APHP231772

Identifier Type: -

Identifier Source: org_study_id