Cardiometabolic Effects of Aerobic Exercise Plus Breaking Sitting

NCT ID: NCT06106464

Last Updated: 2024-02-13

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 14 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2023-06-12
• Study Completion Date: 2024-02-01

Brief Summary

The goal of this randomized crossover clinical trial is to investigate the effects of interrupting prolonged sitting with intermittent walks of equal volume but at different frequencies, in addition to moderate-intensity aerobic exercise, on postprandial serum lipid profile in young adults between the ages of 18-30. The main questions it aims to answer are:

• Do interrupting prolonged sitting with intermittent walks of equal volume but at different frequencies sustain positive cardiometabolic effects after moderate-intensity aerobic exercise?
• If "yes", which frequency of breaks is more effective to provide cardiometabolic benefits?

Participants will complete four conditions on separate days: 1) 6.5 hours of prolonged sitting after 30 minutes of moderate intensity aerobic exercise 2) breaking prolonged sitting with 2 minutes of walking every 30 minutes after 30 minutes of moderate intensity exercise 3) breaking prolonged sitting with 4 minutes of walking every 1-h after 30 minutes of moderate intensity exercise 4) breaking prolonged sitting with 8 minutes of walking every 2-h after 30 minutes of moderate intensity aerobic exercise.

Conditions

Sedentary Behaviour; Sedentary Time

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: OTHER
• Blinding Strategy: SINGLE

Study Groups

Aerobic exercise+6.5 h of prolonged sitting

Participants will sit 6.5 hours after 30 minutes of moderate intensity aerobic exercise.

Group Type: EXPERIMENTAL

Aerobic exercise+6.5 h of prolonged sitting

Intervention Type: OTHER

Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then sit in the chair for 6.5 hours. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.

Aerobic exercise+2 minutes of walking breaks every 30 minutes

Participants will break prolonged sitting with 2 minutes of walking every 30 minutes after 30 minutes of moderate intensity aerobic exercise.

Group Type: EXPERIMENTAL

Aerobic exercise+2 minutes of walking breaks every 30 minutes

Intervention Type: OTHER

Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 2-minute walks at 35-40% of their heart rate reserve every 30 minutes for 6.5 hours after moderate-intensity aerobic exercise. They will make a twelve interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.

Aerobic exercise+4 minutes of walking breaks every 1-h

Participants will break prolonged sitting with 4 minutes of walking every 1-h after 30 minutes of moderate intensity aerobic exercise.

Group Type: EXPERIMENTAL

Aerobic exercise+4 minutes of walking breaks every 1-h

Intervention Type: OTHER

Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 4-minute walks at 35-40% of their heart rate reserve every 1 hour for 6.5 hours after moderate-intensity aerobic exercise. They will make six interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.

Aerobic exercise+8 minutes of walking breaks every 2-h

Participants will break prolonged sitting with 8 minutes of walking every 2-h after 30 minutes of moderate intensity aerobic exercise.

Group Type: EXPERIMENTAL

Aerobic exercise+8 minutes of walking breaks every 2-h

Intervention Type: OTHER

Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 8-minute walks at 35-40% of their heart rate reserve every 2 hour for 6.5 hours after moderate-intensity aerobic exercise. They will make six interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.

Interventions

Aerobic exercise+6.5 h of prolonged sitting

Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then sit in the chair for 6.5 hours. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.

Intervention Type: OTHER

Aerobic exercise+2 minutes of walking breaks every 30 minutes

Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 2-minute walks at 35-40% of their heart rate reserve every 30 minutes for 6.5 hours after moderate-intensity aerobic exercise. They will make a twelve interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.

Intervention Type: OTHER

Aerobic exercise+4 minutes of walking breaks every 1-h

Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 4-minute walks at 35-40% of their heart rate reserve every 1 hour for 6.5 hours after moderate-intensity aerobic exercise. They will make six interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.

Intervention Type: OTHER

Aerobic exercise+8 minutes of walking breaks every 2-h

Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 8-minute walks at 35-40% of their heart rate reserve every 2 hour for 6.5 hours after moderate-intensity aerobic exercise. They will make six interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Being between the ages of 18-30
• Being able to participate in physical activity
• More than 7 hours of daily sitting time measured subjectively
• Not exercising regularly in the last 3 months

Exclusion Criteria

• Hypertension
• Coronary heart disease
• Heart failure
• Diabetes mellitus
• Metabolic syndrome
• Obesity
• Hypothyroidism
• Non-alcoholic fatty liver disease
• Chronic inflammatory joint disease
• Chronic renal failure
• Cushing's syndrome
• HIV infection
• Being on antihypertensive medication
• Being on lipid-lowering medication
• Being on antidiabetic medication
• Being on systemic glucocorticoid medication
• LDL-cholesterol≥130 mg.dL-1
• HDL-cholesterol <40 mg.dL-1 for men; <50 mg.dL-1 for women
• Prediabetes
• Alcohol addiction
• Smoking
• Pregnancy

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 30 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Yasin Ak

OTHER

Sponsor Role: lead

Responsible Party

Yasin Ak

Principal Investigator

Responsibility Role: SPONSOR_INVESTIGATOR

Locations

Kastamonu University, Çatalzeytin Vocational School

Kastamonu, , Turkey (Türkiye)

Countries

Turkey (Türkiye)

References

Ak Y, Guzel Y, Akyel S, Tatar OD, Karabulut E, Karaca A. Hourly 4-minute walking breaks from sitting following aerobic exercise reduce postprandial non-HDL cholesterol in healthy young adults: A randomized crossover trial. J Clin Lipidol. 2025 May-Jun;19(3):638-648. doi: 10.1016/j.jacl.2025.03.007. Epub 2025 Mar 21.

Reference Type: DERIVED

PMID: 40246606 (View on PubMed)

Other Identifiers

LIPA Break

Identifier Type: -

Identifier Source: org_study_id