Multicomponent Analysis of phYsical Frailty BiomarkErs: Focus on Mitochondrial Health - MAYBE

NCT ID: NCT05938205

Last Updated: 2023-07-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

111 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-03-01

Study Completion Date

2022-07-06

Brief Summary

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The study will be conducted in a group of obese sarcopenic patients (Sarco-Ob) aged \> 60 years on moderately hypocaloric diet therapy aimed at comparing the effect of a strength exercise protocol, combined or not with dietary supplementation with amino acids, on physical performance, muscle strength, body composition, muscle oxidative metabolism, and biomarkers of sarcopenia. The supplementation study will be conducted in a double-blind manner.

Detailed Description

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In this double-blind randomised control trial, older men, and women resident in Verona (Italy) will be selected from the Geriatrics Nutritional Clinic of the Borgo Trento Hospital or were contacted by telephone. A specific medical screening was initially programmed to verify the criteria of recruitment.

Anthropometric and body composition In the morning, in a fasted state, body mass will be taken with an electronic scale (Tanita electronic scale BWB-800 MA) and stature will be measured with a Harpenden stadiometer (Holtain Ltd., Crymych, Pembs. UK); BMI will be calculated as weight (kg)/height (m)2. Thereafter, total and regional body composition will be evaluated by means of dual-energy X-ray absorptiometry (DXA) using a total body scanner (QDR Horizon W, Hologic, MA, USA; fan-bean technology, software for Windows XP version 12.4.2) according to the manufacturer's procedures. Furthermore, the ALMI will be calculated as the sum of lean mass of arms and legs divided by height squared.

Physical performance On the same day, the SPPB test will be used to assess functional performance of the lower extremities. Each participant will performed: 4 m at usual customary gait speed, a five repeated chair-stands test and three standing balance tests (side by side, semi-tandem and tandem).

A digital dynamometer (CAMRY, Digital Hand Dynamometer model: EH101) will be used to assess HGS and all measurements were conducted by the same operator. Before starting, participants will observe a demonstration of the test procedure and engage in two practice attempts.

Knee muscle strength measurements and analysis An isokinetic dynamometer (CMSi Cybex Humac Norm Dynamometer, Lumex, Ronkokoma, NY, USA) will be used to assess the isokinetic strength of knee flexors and extensors muscles.

Maximal isokinetic knee extension-flexion tests will be performed in a randomised sequence at 60, 90, 150, 180 and 210°ˑs-1. Trials will consist in three knee extension-flexion movements with 60 s-recovery. The maximal strength of knee extensors muscle will be calculated with the Hill's hyperbolic function. The isokinetic knee extension-flexion power will be calculated as the product between strength and velocity. Finally, for knee extensors, maximal muscle power expressed in Watt, will be calculated from the power-velocity curve.

1-RM assessment Before starting training intervention, participants will perform three sessions of familiarization with the resistance isotonic machines. High intensity strength exercise (Exe-H) protocol: All the participants will performed a supervised progressive resistance training protocol, three times per week, 1 hour per session. Each participant will performed the same strength training protocol, starting with 3 sets of 8-10 repetitions at 70% of 1-RM (month 1), with a progressive increase of intensity at 75% of 1-RM (month 2 and month 3), and 80% 1-RM that will be maintained for the rest period of intervention. The compliance will be define as the number of exercise sessions expressed in % of the target value. Each participant will must reach a compliance above the 70% to be included in the final analysis.

Conditions

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Sarcopenic Obesity Frailty Malnutrition; Protein

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Single-center open-label randomized experimental study with 3 parallel arms with an allocation ratio of 1:1:1. Participants will be all involved in high intensity exercise (Exe-H). Participants will be further randomized into 3 groups to receive (double-blind) dietary supplementation with either placebo, low dose amino acid blend (AA-1) or full dose amino acid blend (AA-2).
Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Investigators
Double blind

Study Groups

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Exe-H + placebo

high intensity physical exercise combined with placebo

Group Type EXPERIMENTAL

Placebo

Intervention Type BEHAVIORAL

Nutritional Placebo

Exe-H

Intervention Type BEHAVIORAL

high intensity physical exercise

Exe-H + AA-1

high intensity physical exercise combined with low dose of aminoacids

Group Type EXPERIMENTAL

AA-1

Intervention Type DIETARY_SUPPLEMENT

low dose aminoacids

Exe-H

Intervention Type BEHAVIORAL

high intensity physical exercise

Exe-H + AA-2

high intensity physical exercise combined with high dose of aminoacids

Group Type EXPERIMENTAL

AA-2

Intervention Type DIETARY_SUPPLEMENT

high dose aminoacids

Exe-H

Intervention Type BEHAVIORAL

high intensity physical exercise

Interventions

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AA-1

low dose aminoacids

Intervention Type DIETARY_SUPPLEMENT

AA-2

high dose aminoacids

Intervention Type DIETARY_SUPPLEMENT

Placebo

Nutritional Placebo

Intervention Type BEHAVIORAL

Exe-H

high intensity physical exercise

Intervention Type BEHAVIORAL

Eligibility Criteria

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Inclusion Criteria

* Older men and women between 60-80 years
* The presence of sarcopenic obesity was considered when the following two conditions were satisfied: Fat mass \> 34.8% and FFM \<90% of subject's ideal FFM.
* Resident in Verona (Italy)
* Stable weight in the previous 2 months
* Previously sedentary (less than one hour of exercise per week in the last 6 months)
* Signing of the informed consent for participation in the study

Exclusion Criteria

* Unstable angina or recent myocardial infarction
* Malignant or unstable arrhythmias
* Heart failure NYHA class \> II
* Severe respiratory failure
* Severe heart valve disease (i.e severe aortic stenosis or insufficiency)
* Abdominal and/or thoracic aneurysm
* Recent intracerebral or subdural haemorrhage
* Poorly controlled arterial hypertension
* Presence of pacemakers or metal prostheses
* Severe chronic renal failure
* Symptomatic musculoskeletal pathology
* Symptomatic disc herniation, arthrosis, acute joint, tendon and ligamentous injuries, hip and/or knee prostheses recently placed (\<6 months) or with joint instability, symptomatic or large inguinal or abdominal hernia
* Acute retinal detachment or bleeding
* Recent eye surgery (laser, cataract, retinal surgery, glaucoma surgery)
* History of malignant cancer within the previous 5 years
* Diagnosis of dementia
* Eating disorders
Minimum Eligible Age

60 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Università degli Studi di Brescia

OTHER

Sponsor Role collaborator

Azienda Ospedaliera di Padova

OTHER

Sponsor Role collaborator

University of Milan

OTHER

Sponsor Role collaborator

Azienda Ospedaliera Universitaria Integrata Verona

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Elena Zoico, PhD

Role: STUDY_DIRECTOR

Universita di Verona

Locations

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AOUI Verona

Verona, , Italy

Site Status

Countries

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Italy

Related Links

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https://www.aovr.veneto.it/web/healthyaging/chi-siamo

Healthy Aging Center Verona web site

Other Identifiers

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1956CESC

Identifier Type: -

Identifier Source: org_study_id

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