Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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UNKNOWN
60 participants
OBSERVATIONAL
2022-12-01
2023-03-01
Brief Summary
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Participants will be divided into two groups: the control group and the air pollution to detect the biomarkers of the Nrf2- dependent ferroptosis pathway.
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Detailed Description
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Methods:
1. The investigators first analyze the genes expression of the GSE60767 from the GEO database (www.ncbi.nlm.nih.gov/geo) using the GPL6947 platform
2. The investigators enrolled 30 people and aged-matched PM2.5 exposures people from two cities (Yangzhou and Xuzhou) as the control and air pollution group based on previous PM2.5 records;
3. Recorded the PM2.5 level in two cities every 5 days between 2022.12 and 2023.2 (winter) to compare the difference;
4. TNF-α ELISA KIT was used to compare the proinflammatory cytokines in two groups
5. 4HNE (4-Hydroxynonenal )ELISA KIT was used to compare the lipid reactive oxygen species level in two groups
6. Quantitative reverse transcription PCR (Polymerase Chain Reaction) was used to compare messenger ribonucleic acid (mRNA) expression of the Nrf2-dependent ferroptosis pathway (Nrf2, SLC7A11, GPX4) Detailed Methods protocol
1\. PM2.5 level source: the PM2.5 levels were recorded via the data from the Ministry of Ecology and Environment of the People's Republic of China (air.cnemc.cn:18007) 2. The criteria of enrolled participants were as follows：Inclusion: living in this city for more than 3 years; no exposure to any chemistry pollution before; Exclusion: significant changes in biomarkers (TNF-α, Nrf2, SLC7A11, GPX4) at the beginning; neurological disorders; endocrine diseases; cancer 3. Sample collection: At the beginning and the end of this study, the enrolled participant will come to the hospital to collect peripheral Blood (Northern Jiangsu and Xuzhou center, respectively), and the blood samples will be collected into EDTA (ethyleneDiamine tetraacetic acid) vacutainer tubes and then divided into leukocytes and plasma for further experiments 4. TNF-α ELISA KIT: Plasma TNF-α concentrations were detected by the competitive ELISA method 5. 4HNE（4-Hydroxynonenal ）ELISA KIT : Plasma 4HNE concentrations were detected by the competitive ELISA method 6. primer sequence: h-GPX4 forward: 5'-GAGGCAAGACCGAAGTAAACTAC-3' h-GPX4 reverse: 5'-CCGAACTGGTTACACGGGAA-3' h-SLC7A11 forward: 5'-TCTCCAAAGGAGGTTACCTGC-3' h-SLC7A11 reverse: 5'-AGACTCCCCTCAGTAAAGTGAC-3' h-Nrf2 forward 5'-ATAGCTGAGCCCAGTATC-3' h-Nrf2 reverse 5'- CATGCACGTGAGTGCTCT-3' h-GAPDH forward: 5'-GGAGCGAGATCCCTCCAAAAT-3' h-GAPDH reverse: 5'-GGCTGTTGTCATACTTCTCATGG-3'
Conditions
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Study Design
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COHORT
CROSS_SECTIONAL
Study Groups
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The control group
the invited volunteers live in Yangzhou, Jiangsu, China (low PM2.5 level based on previous official PM2.5 records)
No interventions assigned to this group
The PM2.5 exposure group
the invited volunteers live in Xuzhou, Jiangsu, China (high PM2.5 level based on previous official PM2.5 records)
No interventions assigned to this group
Eligibility Criteria
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Inclusion Criteria
Exclusion Criteria
18 Years
ALL
Yes
Sponsors
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Xuzhou Medical University
OTHER
Northern Jiangsu People's Hospital
OTHER
Responsible Party
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WenJing Zhao
M.D.; attending doctor
Principal Investigators
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Ju Gao, Phd
Role: STUDY_DIRECTOR
Affiliated Northern Jiangsu People's Hospital of Yangzhou University
Locations
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Wenjing Zhao
Yangzhou, Jiangsu, China
Countries
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Other Identifiers
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NorthernJiangsu-zhao
Identifier Type: -
Identifier Source: org_study_id
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