Absorption and Digestion Kinetics of Human Metabolites

NCT ID: NCT05017428

Last Updated: 2021-08-23

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

5 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-04-08

Study Completion Date

2021-07-01

Brief Summary

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The purpose of this pilot study is to evaluate the effects oral supplementation with four human metabolites (spermidine, nicotinamide, palmitoylethanolamide (PEA), and oleoylethanolamide(OEA)) at varying doses on the circulating blood levels of these metabolites as well as their immediate effects on plasma functionality and postprandial inflammation. 5 young healthy subjects will participate in a four armed study consisting of a Placebo arm and a Low, Medium, and High Dose arm. Subjects will be given a standardized breakfast along with supplementation with either an escalating dose (Low: 1x, Medium: 2x, High: 3x) of a combination of spermidine, nicotinamide, PEA and OEA or a placebo control and a time course of their blood plasma will be collected after supplementation. Plasma samples will be assessed for their concentration of spermidine, nicotinamide, PEA, and OEA as well as their experimental and clinical functionalities including their anti-inflammatory, antioxidant, and cholesterol efflux abilities on primary human macrophage.

Detailed Description

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Conditions

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Postprandial Inflammation

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

CROSSOVER

Participants will take a supplement containing 4 metabolites at escalating doses compared with placebo after a standardized meal and their blood will be drawn at mutliple points throughout a postprandial time course to evaluate markers of inflammation, circulating levels of each metabolite, and plasma functionalities.
Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Low Dose

The Low Dose arm provides subjects with supplementation with 5mg of spermidine (in the form of 5g of a 0.1% spermidine wheat germ extract), 500mg of nicotinamide, 400mg of palmitoylethanolamide, and 200mg of oleoylethanolamide.

Group Type EXPERIMENTAL

Spermidine

Intervention Type DIETARY_SUPPLEMENT

Spermidine in the form of a 0.1% spermidine wheat germ extract

Nicotinamide

Intervention Type DIETARY_SUPPLEMENT

Nicotinamide given as niacinamide

PEA

Intervention Type DIETARY_SUPPLEMENT

Palmitoylethanolamide given at 98% purity

OEA

Intervention Type DIETARY_SUPPLEMENT

Oleoylehtanolamide given at 90% purity

Medium Dose

The Medium Dose arm provides subjects with supplementation with 10mg of spermidine (in the form of 10g of a 0.1% spermidine wheat germ extract), 750mg of nicotinamide, 800mg of palmitoylethanolamide, and 400mg of oleoylethanolamide.

Group Type EXPERIMENTAL

Spermidine

Intervention Type DIETARY_SUPPLEMENT

Spermidine in the form of a 0.1% spermidine wheat germ extract

Nicotinamide

Intervention Type DIETARY_SUPPLEMENT

Nicotinamide given as niacinamide

PEA

Intervention Type DIETARY_SUPPLEMENT

Palmitoylethanolamide given at 98% purity

OEA

Intervention Type DIETARY_SUPPLEMENT

Oleoylehtanolamide given at 90% purity

High Dose

The Low Dose arm provides subjects with supplementation with 15mg of spermidine (in the form of 15g of a 0.1% spermidine wheat germ extract), 1000mg of nicotinamide, 1200mg of palmitoylethanolamide, and 600mg of oleoylethanolamide.

Group Type EXPERIMENTAL

Spermidine

Intervention Type DIETARY_SUPPLEMENT

Spermidine in the form of a 0.1% spermidine wheat germ extract

Nicotinamide

Intervention Type DIETARY_SUPPLEMENT

Nicotinamide given as niacinamide

PEA

Intervention Type DIETARY_SUPPLEMENT

Palmitoylethanolamide given at 98% purity

OEA

Intervention Type DIETARY_SUPPLEMENT

Oleoylehtanolamide given at 90% purity

Placebo

In this arm the participants are given supplementation with a placebo control consisting of 15g of wheat flour.

Group Type PLACEBO_COMPARATOR

Wheat Flour

Intervention Type DIETARY_SUPPLEMENT

Wheat flour given as a placebo control

Interventions

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Spermidine

Spermidine in the form of a 0.1% spermidine wheat germ extract

Intervention Type DIETARY_SUPPLEMENT

Nicotinamide

Nicotinamide given as niacinamide

Intervention Type DIETARY_SUPPLEMENT

PEA

Palmitoylethanolamide given at 98% purity

Intervention Type DIETARY_SUPPLEMENT

OEA

Oleoylehtanolamide given at 90% purity

Intervention Type DIETARY_SUPPLEMENT

Wheat Flour

Wheat flour given as a placebo control

Intervention Type DIETARY_SUPPLEMENT

Other Intervention Names

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Primeadine Jarrow Niacinamide Nootropics Depot PEA Riduzone

Eligibility Criteria

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Inclusion Criteria

* Age: 20-40 years old to constitute a young study population
* BMI: 19-27 kg/m2 to constitute a normal/healthy weight population
* Weight: 133lbs or more
* Subjects must be willing and able to consume a standardized breakfast bar containing cashews and dates (Larabar).
* Subjects must be willing to consume oral dietary supplement versions of spermidine, 1-methlynicotinamide, palmitoylethanolamide, and oleoylethanolamide.
* Subjects must be willing and able to undergo blood draws at the UC Davis Ragle Nutrition Center at 0, 1, 2, and 4 hours after supplementation with spermidine, niacinamide, palmitoylethanolamide, and oleoylethanolamide.

Exclusion Criteria

* Smoker
* Anemia
* Documented chronic diseases including diabetes, thyroid disease, metabolic syndrome, cancer, autoimmune diseases, or previous cardiovascular events
* Any inflammatory bowel disease including IBS, Crohn's Disease, Celiac Disease
* Any allergy or sensitivity to wheat, gluten, or soy products
* Consumption of \>1 alcoholic drink/day
* Current consumption of any probiotic, prebiotic, or dietary supplements
* Extreme dietary or exercise patterns
* Recent weight fluctuations (greater than 10% in the last six months)
* Regular use of over-the-counter allergy or pain medications (\>1/week)
* Taking prescription lipid medications (e.g. statins) or other supplements known to alter lipoprotein metabolism such as isoflavones.
* Use of any form of hormonal birth control including oral contraception pills, hormonal IUDs or rings, or hormonal birth control patches
* Illness such as flu or cold of any kind within the last two weeks
* Allergy to components of standardized breakfast bar (cashew/dates)
* Changes to any of the above during the study period.
Minimum Eligible Age

20 Years

Maximum Eligible Age

40 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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University of California, Davis

OTHER

Sponsor Role lead

Responsible Party

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Angela Zivkovic, PhD

Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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University of California Davis

Davis, California, United States

Site Status

Countries

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United States

References

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Rhodes CH, Hong BV, Tang X, Weng CY, Kang JW, Agus JK, Lebrilla CB, Zivkovic AM. Absorption, anti-inflammatory, antioxidant, and cardioprotective impacts of a novel fasting mimetic containing spermidine, nicotinamide, palmitoylethanolamide, and oleoylethanolamide: A pilot dose-escalation study in healthy young adult men. Nutr Res. 2024 Dec;132:125-135. doi: 10.1016/j.nutres.2024.10.006. Epub 2024 Oct 23.

Reference Type DERIVED
PMID: 39549554 (View on PubMed)

Other Identifiers

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1725738

Identifier Type: -

Identifier Source: org_study_id

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