Sperm Selection and Embryo Development: Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods Sperm Selection and Embryo Development: Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods

NCT ID: NCT04962074

Last Updated: 2021-07-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

22 participants

Study Classification

INTERVENTIONAL

Study Start Date

2020-01-05

Study Completion Date

2020-09-20

Brief Summary

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Purpose: To compare the effects of the density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in a study population with astheno-teratozoospermia.

Materials and Methods: In this prospective study, the semen samples of the patients with astheno-teratozoospermia were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were used to fertilize the mature sibling oocytes. Semen parameters and embryo development on days 3 and 5 were assessed.

Detailed Description

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Conditions

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Reduced Sperm Motility IVF ICSI Astheno Teratozoospermia

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Participants

Study Groups

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Density gradient method

Sperm prepared by density gradient method

Group Type EXPERIMENTAL

Density gradient

Intervention Type DEVICE

Density gradient centrifugation

Microfluidic chip

Sperm prepared by microfluidic chip method

Group Type EXPERIMENTAL

Microfluidic sperm sorting chip

Intervention Type DEVICE

Fertile Ultimate® (Koek Biotechnology, Turkey) microfluidic sperm sorting chips

Interventions

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Microfluidic sperm sorting chip

Fertile Ultimate® (Koek Biotechnology, Turkey) microfluidic sperm sorting chips

Intervention Type DEVICE

Density gradient

Density gradient centrifugation

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* Infertile men with astheno-teratozoospermia
* Women were aged from 18 to 39
* Women had at least two mature oocytes.

Exclusion Criteria

* MI oocytes
Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Maltepe University

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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MaltepeU

Istanbul, , Turkey (Türkiye)

Site Status

Countries

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Turkey (Türkiye)

Other Identifiers

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2019

Identifier Type: -

Identifier Source: org_study_id

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