Semen Processing for ICSI Using Swim-down and Density Gradient Methods
NCT ID: NCT02328534
Last Updated: 2014-12-31
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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COMPLETED
890 participants
OBSERVATIONAL
2007-03-31
2014-01-31
Brief Summary
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Detailed Description
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The routine used method as density gradient (DG) includes about 30 min centrifugation at 1200 RPM, and more working steps with more handling procedures. According to many references the investigators can approve that it is method to be indicated with all cases particularly with those severely decreases semen number or motility.
Using 100 % silica solution for semen processing used for ICSi, shews lesser centrifugation time and minimum processing procedures. As swimming down time is about 30 min followed by 5 min washing and finalized with other 3 minutes last wash. For that total interfering physical action is about 8 minutes compared with 30 min with DG method.
In swimming down we are using the natural sperm behavior and own swimming power for separate itself from other seminal components. As well as only the one normally structure and motility direction the only can penetrate the interface surface of silica solution and swim inside higher density matrix using its speed and power.
Conditions
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Keywords
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Study Design
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COHORT
RETROSPECTIVE
Study Groups
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Density Gradient semen
ICSI Cases processed using DG semen processing and evaluated by
Blastocyst Formation Rate Blastocyst Rate Pregnancy Rate
Blastocyst Formation Rate
Embryos developed at Day3 cultures, are continued for Day5 culture tho reach blastocyst stage. The percentage of blastocyst produced from cleaved embryos to be evaluated ar rate.
The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
Blastocyst Rate
Day 5 embryo transfer achieved pregnancy are compared with total embryo transfer cases at Day5.
The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
Pregnancy confirmed with day 12 after Et and repeated with 48 hours after 1st exam.
Pregnancy Rate
All pregnancy achieved from all ET. Pregnancy confirmed with day 12 after Et and repeated with 48 hours after 1st exam.
The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
Swimming down semen
ICSI Cases processed using SD semen processing and evaluated by
Blastocyst Formation Rate Blastocyst Rate Pregnancy Rate
Blastocyst Formation Rate
Embryos developed at Day3 cultures, are continued for Day5 culture tho reach blastocyst stage. The percentage of blastocyst produced from cleaved embryos to be evaluated ar rate.
The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
Blastocyst Rate
Day 5 embryo transfer achieved pregnancy are compared with total embryo transfer cases at Day5.
The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
Pregnancy confirmed with day 12 after Et and repeated with 48 hours after 1st exam.
Pregnancy Rate
All pregnancy achieved from all ET. Pregnancy confirmed with day 12 after Et and repeated with 48 hours after 1st exam.
The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
Interventions
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Blastocyst Formation Rate
Embryos developed at Day3 cultures, are continued for Day5 culture tho reach blastocyst stage. The percentage of blastocyst produced from cleaved embryos to be evaluated ar rate.
The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
Blastocyst Rate
Day 5 embryo transfer achieved pregnancy are compared with total embryo transfer cases at Day5.
The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
Pregnancy confirmed with day 12 after Et and repeated with 48 hours after 1st exam.
Pregnancy Rate
All pregnancy achieved from all ET. Pregnancy confirmed with day 12 after Et and repeated with 48 hours after 1st exam.
The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
Other Intervention Names
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Eligibility Criteria
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Inclusion Criteria
* Cases have semen examination criteria fall within WHO 2010 standards of motility
* Cases have semen examination criteria with all types of abnormal forms
* Cases produced more than 4 mature oocytes (M2) and more
Exclusion Criteria
* Cases have semen examination criteria with count less than 1.0 million
* Cases with TESE, TESA and EPSA, both fresh and frozen
* Cases produced less than 4 mature oocytes (M2)
20 Years
38 Years
ALL
No
Sponsors
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Ahmad Mustafa Mohamed Metwalley
OTHER
Responsible Party
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Ahmad Mustafa Mohamed Metwalley
Novel Approach of Semen Preparation to Improve ICSI Outcome
Principal Investigators
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Ahmad M Metwalley, Ms. Genetics
Role: STUDY_DIRECTOR
Adliyah
Locations
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Al Baraka Fertility Hospital
Manama, Adliyah, Bahrain
Ibn Sina IVF Center- Ibn Sina Hospital
Sohag, Sohag Governorate, Egypt
Countries
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References
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Malvezzi H, Sharma R, Agarwal A, Abuzenadah AM, Abu-Elmagd M. Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial. Reprod Biol Endocrinol. 2014 Dec 2;12:121. doi: 10.1186/1477-7827-12-121.
Related Links
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Density Gradient procedures description
Other Identifiers
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AlBarakaSD1
Identifier Type: -
Identifier Source: org_study_id