Culture Media Microdroplet Geometry With Different Oil Overlay and Its Effect on Embryonic Development.

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

100 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-08-01

Study Completion Date

2020-01-10

Brief Summary

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A prospective randomized study included 1695 MII sibling oocytes collected from 100 patients undergoing ICSI.

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Detailed Description

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Purpose:

to determine the effect of culture media microdroplet geometry of different culture dishes and oil overlay on the pre implantation embryo development in dry incubators

Method:

We used sibling oocytes splits between two different dishes : GPS (life global, USA) and SPL (life science, Korea) with two different oils : light mineral oil (LM)(Irvine, USA) and paraffin oil (PO)(Vitro life, Sweden). All embryos cultured in dry incubators at the same conditions.

statistical analysis done using SPSS version 23

Conditions

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Embryonic Development Infertility

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Effect of two different culture dishes with two different oil overlay which used in human embryos culturing through ICSI procedures on the pre implantaion embryonic development.

Primary Study Purpose

OTHER

Blinding Strategy

NONE

Study Groups

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GPS / LM versus SPL/LM

to determine the effect of micro droplet geometry in 3D dishes (GPS) or 2D dishes (SPL) in combination with light mineral oil (LM).

Group Type OTHER

GPS/LM

Intervention Type OTHER

Media microdroplet geometry (3D dishes) in combination with light mineral oil overlay

SPL/LM

Intervention Type OTHER

Media microdroplet geometry (2D dishes) in combination with light mineral oil overlay

GPS/PO versus SPL/PO

to determine the effect of micro droplet geometry in 3D dishes (GPS) or 2D dishes (SPL) in combination with paraffin oil (PO).

Group Type OTHER

GPS/PO

Intervention Type OTHER

Media microdroplet geometry (3D dishes) in combination with Paraffin oil overlay

SPL/PO

Intervention Type OTHER

Media microdroplet geometry (2D dishes) in combination with paraffin oil overlay

GPS/PO versus GPS/LM

to determine the effect of oil overlay light mineral(LM) or paraffin oil (PO) in combination with paraffin oil 3D dishes (GPS).

Group Type OTHER

GPS/LM

Intervention Type OTHER

Media microdroplet geometry (3D dishes) in combination with light mineral oil overlay

GPS/PO

Intervention Type OTHER

Media microdroplet geometry (3D dishes) in combination with Paraffin oil overlay

SPL/PO versus SPL/LM

to determine the effect of oil overlay light mineral(LM) or paraffin oil (PO) in combination with paraffin oil 2D dishes (SPL).

Group Type OTHER

SPL/LM

Intervention Type OTHER

Media microdroplet geometry (2D dishes) in combination with light mineral oil overlay

SPL/PO

Intervention Type OTHER

Media microdroplet geometry (2D dishes) in combination with paraffin oil overlay

Interventions

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GPS/LM

Media microdroplet geometry (3D dishes) in combination with light mineral oil overlay

Intervention Type OTHER

SPL/LM

Media microdroplet geometry (2D dishes) in combination with light mineral oil overlay

Intervention Type OTHER

GPS/PO

Media microdroplet geometry (3D dishes) in combination with Paraffin oil overlay

Intervention Type OTHER

SPL/PO

Media microdroplet geometry (2D dishes) in combination with paraffin oil overlay

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Female age is ≤ 37 years old with ≥ 8 MII oocytes
* Males with normal semen parameters (WHO 2010).
* We conducted three experiments all with Siblings oocytes splits and all embryos