SARS-CoV2 (COVID-19) Diagnosis in Human Saliva by MALDI-TOF MS Profiling

NCT ID: NCT04460638

Last Updated: 2023-09-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

779 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-07-23

Study Completion Date

2021-08-13

Brief Summary

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Since March 2020, SARS-CoV2 virus (nCoV19; COVID-19) is considered pandemic. Its high rate of spread and infection in the human population and the lack of effective and validated treatment have led the authorities of several countries to confine their populations to slow the spread of COVID-19. As part of the management of this health crisis, the screening of individuals is essential in order to isolate "infected cases". These screening tests are currently performed on nasopharyngeal swabs using RT-PCR for the detection of viral RNA. Although sensitive and specific, these tests remain relatively long (2-5 hours), expensive and the strong international demand for nucleic extraction kits and enzymes are factors limiting the implementation of widespread screening (problem of supply of swabs, molecular biology consumables).

In order to prevent the risks of a shortage of screening means, we propose to develop an innovative alternative strategy, PCR-free, based on the detection of specific protein signatures in human saliva by MALDI-TOF MS profiling. MALDI-TOF MS profiling is a method used in routine diagnostics by microbiology laboratories for the identification of microorganisms. MALDI-TOF MS profiling has been successfully used to classify individuals according to their infectious status (oral pathologies) based on the analysis of their saliva, but also as a tool for the identification of respiratory viruses from cell culture supernatants. In addition, we have expertise and skills in the field of MALDI-TOF MS profiling and have implemented new strategies to improve the quality of profiles and their analysis, particularly in the context of entomological and vector identification projects. Finally, recent Chinese studies have reported that COVID-19 was detectable in saliva by RT-PCR.

The main objective of this study is to develop a test based on the MALDI-TOF profiling method to detect individuals infected with SARS-CoV2 from saliva sample.

Detailed Description

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Conditions

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SARS-CoV 2

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Covid+ hospitalization group

Patients hospitalized with SARS-CoV2 infection

Saliva collection

Intervention Type BIOLOGICAL

Saliva samples are regularly collected over a period of up to 30 consecutive days.

Clinical assessment

Intervention Type OTHER

The participant's clinical data will be collected regularly over a period of up to 30 consecutive days.

Covid+ outpatient group

Patients or caregivers followed on an outpatient basis for an SARS-CoV2 infection

Saliva collection

Intervention Type BIOLOGICAL

Saliva samples are regularly collected over a period of up to 30 consecutive days.

Clinical assessment

Intervention Type OTHER

The participant's clinical data will be collected regularly over a period of up to 30 consecutive days.

Covid- group

Caregivers not infected with an SARS-CoV2

Saliva collection

Intervention Type BIOLOGICAL

Saliva samples are regularly collected over a period of up to 30 consecutive days.

Clinical assessment

Intervention Type OTHER

The participant's clinical data will be collected regularly over a period of up to 30 consecutive days.

Non-SARS pathology group

Individuals not infected with SARS-CoV2 but with another acute and/or infectious non-SARS pathology

Saliva collection

Intervention Type BIOLOGICAL

Saliva samples are regularly collected over a period of up to 30 consecutive days.

Clinical assessment

Intervention Type OTHER

The participant's clinical data will be collected regularly over a period of up to 30 consecutive days.

Interventions

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Saliva collection

Saliva samples are regularly collected over a period of up to 30 consecutive days.

Intervention Type BIOLOGICAL

Clinical assessment

The participant's clinical data will be collected regularly over a period of up to 30 consecutive days.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* 18 years of age;
* screened for SARS-CoV2 by RT-PCR from a nasopharyngeal swab within 96 hours prior to inclusion.

Exclusion Criteria

* Patient admitted to intensive care;
* Pregnant woman.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Direction Centrale du Service de Santé des Armées

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Hôpital d'Instruction des Armées Laveran

Marseille, , France

Site Status

Countries

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France

Other Identifiers

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2020-A01249-30

Identifier Type: OTHER

Identifier Source: secondary_id

2020-COVID19-15

Identifier Type: -

Identifier Source: org_study_id

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