Evaluation of Novel Diagnostic Tests for COVID-19

NCT ID: NCT04326387

Last Updated: 2020-05-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

200 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-04-06

Study Completion Date

2021-10-07

Brief Summary

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COVID-19 (also known as Coronavirus) originated in the Wuhan China and has since spread to at least 159 countries around the world. It was declared a pandemic by the World health organisation on the 11th of March 2020. The cases in the United Kingdom continue to increase exponentially with up to 5 683 people diagnosed as on the 22nd of March 2020. It is estimated that 1 in 5 people diagnosed will require hospital admission and 1 in 20 intensive care treatment. By developing and improving diagnostic testing, we can accurately diagnose infected cases to triage appropriate treatments, identify individuals for quarantine in order to prevent transmission and obtain information regarding patient's immune systems.

At present, the diagnostic test is a highly specific method of genetic amplification called 'Reverse Transcription - Polymerase Chain Reaction' or RT-PCR, which allows detection of very small amounts of genetic mutations caused by the COVID-19 virus. However, this method must be completed in highly specialised facilities, which are few and far between, increasing time to diagnosis (currently 48-72 hours), increasing exposure to non-infected individuals, and overburdening the analysing facilities. The ideal solution is a point of care (POC) test that can give results immediately. This study aims to harness the point of care technology of the SAMBA II device (Diagnostics for the Real World Ltd.), which is a CE-marked device that has been used with success in the identification of Human Immunodeficiency Virus (HIV), by amplifying genetic material without the need to increase and decrease temperatures during the amplification process.

In the COVIDx study, 200 patients meeting the Public Health England's (PHE) inpatient definition of having suspected COVID-19 will be approached, consented and a sample from throat and nasal swab (combined) or tracheal fluid taken and tested using the SAMBA II method. A combination of the standard PHE RT-PCR and an additional validated laboratory PCR technique will be used as a control in line with standard clinical practice. Patients will undergo an additional serum tests on existing samples as made available after routine clinical assessments to monitor antibody response. Patients will be followed for clinical outcomes at 28 days post-admission.

Detailed Description

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Conditions

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Acute Disease Coronavirus Respiratory Viral Infection

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Research Participants (Patients)

Inpatients symptomatic of suspected COVID-19 Baseline swab of nose/throat, nasopharyx, or endotracheal tube aspirate. SAMBA II point of care test on this swab.

Standard of care bloods taken for PHE and additional confirmatory diagnostic PCR assessment.

Serum antibody tests on any excess blood tests during inpatient stay for immune response monitoring.

Outcome assessment at 1 month

SAMBA II (Diagnostic for the Real World)

Intervention Type DIAGNOSTIC_TEST

Point of care Isothermal-PCR Viral RNA Amplication for virus detection

Public Health England Gold Standard

Intervention Type DIAGNOSTIC_TEST

Reverse Transcription PCR

Cambridge Validated Viral Detection Method

Intervention Type DIAGNOSTIC_TEST

Reverse Transcription PCR

Radiological Detection

Intervention Type DIAGNOSTIC_TEST

Chest X-ray \& CT Scan detection of viral infection in the lungs

Interventions

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SAMBA II (Diagnostic for the Real World)

Point of care Isothermal-PCR Viral RNA Amplication for virus detection

Intervention Type DIAGNOSTIC_TEST

Public Health England Gold Standard

Reverse Transcription PCR

Intervention Type DIAGNOSTIC_TEST

Cambridge Validated Viral Detection Method

Reverse Transcription PCR

Intervention Type DIAGNOSTIC_TEST

Radiological Detection

Chest X-ray \& CT Scan detection of viral infection in the lungs

Intervention Type DIAGNOSTIC_TEST

Other Intervention Names

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Chest X-ray, CT Scan

Eligibility Criteria

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Inclusion Criteria

* 16 years or above
* Patient requiring hospital admission AND AT LEAST ONE OF
* Clinical or Radiological evidence of pneumonia
* Acute respiratory distress syndrome
* Influenza like illness (defined as fever \>37.8oC and at least one of the following respiratory symptoms, which much be of acute onset: persistent cough (with or without sputum), hoarseness, nasal discharge or congestion, shortness of breath, sore throat, wheezing, sneezing)

Exclusion Criteria

* Patients who have not had the standard PHE COVID-19 test applied
* Unwilling or unable to comply with research swab of nose \& throat or tracheal fluid
Minimum Eligible Age

16 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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CCTU- Cancer Theme

OTHER

Sponsor Role lead

Responsible Party

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CCTU- Cancer Theme

Prof. Ravindra Gupta

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Ravindra Prof. Gupta, BMBCh

Role: PRINCIPAL_INVESTIGATOR

University of Cambridge & Cambridge University Hospitals NHS Foundation Trust

Locations

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Cambridge University Hospitals NHS Foundation Trust

Cambridge, Cambridgeshire, United Kingdom

Site Status RECRUITING

Countries

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United Kingdom

Central Contacts

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Richard D. Skells, BSc.

Role: CONTACT

01223 349707 ext. 349707

CCTU Cancer

Role: CONTACT

01223 216038 ext. 216083

Facility Contacts

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Ravi K Gupta, PhD

Role: primary

07500792984

Dami Collier, MBBS

Role: backup

07888707113

Other Identifiers

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COVIDx

Identifier Type: -

Identifier Source: org_study_id

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