Resuscitation Failure, Oxidative Stress, and Necroptosis as Mortality Predictor in Septic Patient

NCT ID: NCT04169412

Last Updated: 2019-11-20

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

72 participants

Study Classification

OBSERVATIONAL

Study Start Date

2019-02-22

Study Completion Date

2019-08-20

Brief Summary

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Resuscitation failure, oxidative stress and necroptosis were able to predict mortality in septic patient

Detailed Description

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This prospective cohort study was conducted at resuscitation room and ICU of Mohammad Hoesin hospital, a single tertiary teaching hospital in Palembang, South Sumatera. This study was started after ethical and location authorization were unleashed in February to August 2019. Inclusion criteria were patients aged 18 years old or above who met Sepsis-3 definition and diagnosed with sepsis. Exclusion criteria were patients whose family did not give any consent to participate, not treated at the ICU, had a late diagnosis (\>24 h), pregnant, and diagnosed with brain dead. Drop out criteria including patients who died \<4 h after diagnosed and could not be followed in 28 days. Investigators were trained to identified all eligible subjects. All the subjects had a standard resuscitation and their blood was taken to be examined at the laboratory. Subjects were observed in 28 days whether there were any mortality or not. Failed resuscitation defined by examined lactate level ≥ 2 mmol/L or lactate reduction \<20%. Data was statistically analyzed with STATA™

Conditions

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Sepsis

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Resuscitation Failure, High PCO Level, High RIPK3 Level

1. Resuscitation failure was defined as lactate level ≥2 mmol/L or lactate reduction \<20% hour-4 after initial sepsis recognition.
2. High PCO level was defined as PCO level ≥ cut off point.
3. High RIPK3 level was defined as PCO level ≥ cut off point.

Blood Collection

Intervention Type DIAGNOSTIC_TEST

Blood Gas Analysis using Stat Profile pHOx® Series (nova biomedical, Waltham, U.S.) and Enzym-linked Immunosorbent Assay (ELISA) using Bio-Rad (Bio-Rad Laboratories, California, U.S.)

Resuscitation Success, Low PCO Level, Low RIPK3 Level

1. Resuscitation success was defined as lactate level \<2 mmol/L or lactate reduction ≥20% hour-4 after initial sepsis recognition.
2. Low PCO level was defined as PCO level \< cut off point.
3. Low RIPK3 level was defined as PCO level \< cut off point.

Blood Collection

Intervention Type DIAGNOSTIC_TEST

Blood Gas Analysis using Stat Profile pHOx® Series (nova biomedical, Waltham, U.S.) and Enzym-linked Immunosorbent Assay (ELISA) using Bio-Rad (Bio-Rad Laboratories, California, U.S.)

Interventions

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Blood Collection

Blood Gas Analysis using Stat Profile pHOx® Series (nova biomedical, Waltham, U.S.) and Enzym-linked Immunosorbent Assay (ELISA) using Bio-Rad (Bio-Rad Laboratories, California, U.S.)

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Participants aged 18 years old or above
* Diagnosed sepsis using Sepsis-3 definition

Exclusion Criteria

* Participants whose family did not give any consent to participate
* Not treated at the ICU
* Had a late diagnosis (\>24 h)
* Pregnant
* Brain dead
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Indonesia University

OTHER

Sponsor Role collaborator

Universitas Sriwijaya

OTHER

Sponsor Role lead

Responsible Party

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Mayang Indah Lestari

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Mohammad Hoesin Central General Hospital

Palembang, South Sumatera, Indonesia

Site Status

Countries

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Indonesia

References

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Other Identifiers

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047/kepkrsmhfkunsri/2019

Identifier Type: -

Identifier Source: org_study_id

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