Chronic Periodontitis Microbiota in Type 2 Diabetes Mellitus Patients

NCT ID: NCT03786133

Last Updated: 2018-12-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

40 participants

Study Classification

OBSERVATIONAL

Study Start Date

2016-06-10

Study Completion Date

2016-11-16

Brief Summary

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This study is a retrospective comparative evaluation of six main periodontal pathogens and total bacterial load in chronic periodontitis patients affected or not by type 2 diabetes mellitus by polymerase chain reaction analysis.

Detailed Description

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The aim of this study was to compare prevalence and bacterial load of six main periodontal pathogens among chronic periodontitis patients with and without type 2 diabetes mellitus. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans genotypes were also investigated.

The recruitment for the study sample was performed analysing the medical records of adult patients with chronic periodontitis visited between 2010 and 2016. Eligible patients were subdivided in two balanced groups on the basis of the presence or absence of type 2 diabetes mellitus at the time of the microbiological test.

For each subject, the following data have been collected: age, gender, smoking habits, type of metabolic control of diabetes, duration of diabetes, number of missing teeth.

For each subject, four periodontal sites were studied. Probing pocket depth, sites with bleeding on probing, sites with suppuration and microbiological data were evaluated.

An individual matching on the basis of severity and extension of periodontitis was performed by pairing a diabetic patient with another non-diabetic one with the same degree of severity and extension of the periodontal disease.

Microbiological data of subgingival biofilm were analysed and compared for the examined pathogens: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola, Fusobacterium nucleatum, Tannerella forsythia.

Differences in genotypes of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans among diabetic and non-diabetic patients were also evaluated.

Conditions

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Chronic Periodontitis Type 2 Diabetes Mellitus

Keywords

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Subgingival analysis Real-time PCR Comparative study Chronic Periodontitis Type 2 Diabetes Mellitus

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

RETROSPECTIVE

Study Groups

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type 2 diabetics

Analysis of microbiological tests in chronic periodontitis patients with type 2 diabetes mellitus

Analysis of microbiological tests

Intervention Type DIAGNOSTIC_TEST

Collection and comparison of microbiological data

non-diabetics

Analysis of microbiological tests in chronic periodontitis patients without type 2 diabetes mellitus

Analysis of microbiological tests

Intervention Type DIAGNOSTIC_TEST

Collection and comparison of microbiological data

Interventions

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Analysis of microbiological tests

Collection and comparison of microbiological data

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* diagnosis of chronic periodontitis
* diagnosis of type 2 diabetes mellitus (only in the test group)
* the presence of at least 12 teeth (except for the third molar)
* belonging to caucasian ethnic group
* age greater than 18 years.

Exclusion Criteria

* pregnant or lactating
* history of systemic antibiotics within 3 months prior to the microbiological testing or anti-inflammatory therapy in the month before the visit
* if they were suffering from any other systemic disease except diabetes mellitus (arthritis, ulcerative colitis, Crohn's disease, osteoporosis or osteopenia, HIV infection, hematologic diseases, neoplastic diseases, cardiovascular diseases or pathologies that could potentially interfere with the periodontitis and/or with diabetes)
* mental disorders
* if they had received periodontal treatment in the 6 months before the microbiological test.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University of Bologna

OTHER

Sponsor Role lead

Responsible Party

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Marco Montevecchi

Aggregate Professor of Periodontology

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Marco Montevecchi, DDS

Role: STUDY_CHAIR

University of Bologna

Locations

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Alma Mater Studiorum - University of Bologna, Department of Biomedical and Neuromotor Sciences, School of Dentistry - Division of Periodontology and Implantology.

Bologna, , Italy

Site Status

Countries

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Italy

References

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Montevecchi M, Valeriani L, Gatto MR, D'Alessandro G, Piana G. Subgingival pathogens in chronic periodontitis patients affected by type 2 diabetes mellitus: a retrospective case-control study. J Periodontal Implant Sci. 2021 Dec;51(6):409-421. doi: 10.5051/jpis.2100180009.

Reference Type DERIVED
PMID: 34965620 (View on PubMed)

Other Identifiers

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periopathogensdiabetes

Identifier Type: -

Identifier Source: org_study_id