Effect of Exercise on Genes That Control Muscle Function

NCT ID: NCT03414385

Last Updated: 2023-03-23

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 16 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2018-02-01
• Study Completion Date: 2022-05-31

Brief Summary

The proposed project will examine how exercise counteracts metabolic disorders and type 2 diabetes through regulating gene expression. The project is highly relevant to public health because of the global pandemic of diabetes, obesity, and associated metabolic syndromes as well as the well-known metabolic benefit of physical exercise in correcting these disorders.

Detailed Description

Exercise is a first-line treatment for type 2 diabetes, and exerts its beneficial effects not only by burning off energy but also by causing prolonged metabolic changes through changing gene expression. Genes are our genetic materials and the expression of genes determines our biology. In our previous study in animals, we identified some factors that drive exercise-induced gene expression changes. Here we would like to address whether the result is also true in human. This work will provide molecular insights into how exercise remodels our metabolism and will potentially find a way to maximize the benefit we get from physical exercise.

The purpose of this study is to determine whether acute exercise activate certain molecular factors in human skeletal muscle. Participants will be asked to undergo an acute bout of aerobic exercise at ~ moderate intensity for about 2 hours. Before and after the exercise, the participants will undergo a muscle biopsy. The muscle tissues will be used for total RNA extraction and RT-qPCR analysis of genes that include but are not limited to de facto JunD/AP-1 target genes and will also be analyzed by Jun D Chip-qPCT to assess binding of Jun D on its de facto target genes.

Conditions

Healthy

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: PREVENTION
• Blinding Strategy: NONE

Study Groups

Exercise group

Research volunteers will be asked to undergo an acute bout of aerobic exercise at moderate intensity for \~120 minutes. Before and after the exercise the volunteer will undergo leg biopsy.

Group Type: EXPERIMENTAL

Exercise

Intervention Type: OTHER

Acute bout of aerobic exercise at moderate intensity for 2 hours

Interventions

Exercise

Acute bout of aerobic exercise at moderate intensity for 2 hours

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Age 20 - 40 years
• BMI 18.5 - 29.9
• Stable body weight (not more than 2 kg change) during the past 6 months
• Moderate sedentary (regular exercise less than 1 hour per week for the last 6 months)
• Willing to provide informed consent

Exclusion Criteria

• Failure to provide informed consent
• Any major chronic disease or any condition that would interfere with exercise, in which exercise is contraindicated, or that would interfere with interpretation of results
• Severe orthopedic and or neuromuscular disease that would contraindicate participation in exercise
• Other significant co-morbid disease that would impair ability to exercise
• Uncontrolled hypertension (BP greater than 160/90)
• History of malignancy during the past 5 years
• Diabetes mellitus as determined by self-report with verification (medical records, current treatment, confirmation from health care provider), or HbA1c of exceeding 6.5%

• Minimum Eligible Age: 20 Years
• Maximum Eligible Age: 40 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)

NIH

Sponsor Role: collaborator

US Department of Veterans Affairs

FED

Sponsor Role: collaborator

Baylor College of Medicine

OTHER

Sponsor Role: lead

Responsible Party

Dennis T. Villareal

Professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Dennis T Villareal, MD

Role: PRINCIPAL_INVESTIGATOR

Baylor College of Medicine

Zheng Sun, PhD

Role: PRINCIPAL_INVESTIGATOR

Baylor College of Medicine

Locations

Baylor College of Medicine/Michael E DeBakey VA Medical Center

Houston, Texas, United States

Countries

United States

Other Identifiers

1R01DK111436-01A1

Identifier Type: NIH

Identifier Source: secondary_id

View Link

H-40366

Identifier Type: -

Identifier Source: org_study_id