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DNA Methylation Profile of the SOCS-1 Gene Promoter in Smokers Patients With Chronic Periodontitis.

NCT ID: NCT03371446

Last Updated: 2017-12-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

60 participants

Study Classification

OBSERVATIONAL

Study Start Date

2016-06-06

Study Completion Date

2017-11-18

Brief Summary

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Periodontitis is related to host genetics, constitution of the dental biofilm and environmental factors such as smoking. DNA methylation is a mechanism of genetic expression that can inhibit or silence gene expression. In this way several researchers have been dedicated to study the genetic influence on the susceptibility and / or increased risk to periodontal disease. Studies have reported association between several epigenetic biomarkers with periodontal inflammation. Considering the hypothesis that there is an association between smoking and methylation in genes related to periodontal disease, the objective of this study was to verify the DNA methylation pattern in oral epithelial cells of patients with chronic periodontitis (CP) in the promoter of a specific gene involved in the control of inflammation, as suppressor of cytokine signaling (SOCS) 1 in smokers and nonsmokers patients.

Detailed Description

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This was an experimental type study with parallel controls, comparing two groups, a group with consumption of 10 minimum cigarettes per day, with a diagnosis of chronic periodontitis. And another control group were non-smokers with chronic periodontitis. For this, genomic DNA was purified from oral epithelial cells obtained by rinsing with 3% sucrose, for a single time of collection, patients received periodontal treatment after collection and data analysis. The DNA was modified by Sodium bisulfite and the methylation patterns of the DNA were analyzed with the MSPCR technique (Polymerase chain reaction). This study was approved by the Institutional Review Board of the School of Dentistry of Ribeirão Preto (CAAE:57171816.2.0000.5419 ), and all patients need to provide written informed consent.

Conditions

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Periodontal Diseases Epigenetics

Keywords

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DNA Methylation. Smoking. SOCS-1 gene.

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

OTHER

Study Groups

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Smokers/Non-smokers

It was an experimental study with parallel controls, comparing two groups, a group with patients who smoked for more than 10 years, consuming 10 more cigarettes per day and diagnosing chronic periodontitis (case) and another group (control) were non-smokers with chronic periodontitis, according to the standard of World Health Organization (WHO) definition of the smoking population

oral epithelial cells obtained by rinsing with 3% sucrose

Intervention Type GENETIC

rinsing with 3% sucrose, for a single time of collection, patients received periodontal treatment after collection and data analysis.

Interventions

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oral epithelial cells obtained by rinsing with 3% sucrose

rinsing with 3% sucrose, for a single time of collection, patients received periodontal treatment after collection and data analysis.

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

* Chronic Periodontitis carriers - presence of proximal insertion loss ≥5 mm in more than 30% of the teeth present.
* Periodontal pocket ≥ 5mm
* Smokers who have smoked 10 or more cigarettes per day for the past five years.
* No positive history of basic periodontal treatment in the last six months.

Exclusion Criteria

* Extensive prosthetic involvement.
* Using anti-inflammatories.
* Pregnant or nursing.
* Presence of systemic alterations that compromise host response or require prophylactic medication for treatment.
* History of constant use of oral antiseptics in the last six months.
Minimum Eligible Age

30 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Sao Paulo

OTHER

Sponsor Role lead

Responsible Party

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Arthur Belem Novaes Jr

Teacher of Periodontia- Department of Buco Maxillofacial Surgery and Traumatology and Periodontics

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Arthur B. Novaes Junior, DDS, PhD

Role: PRINCIPAL_INVESTIGATOR

Study Principal Investigator

Locations

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Arthur Belem Novaes Junior

Ribeirão Preto, São Paulo, Brazil

Site Status

Countries

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Brazil

Other Identifiers

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CAAE: 57171816.2.0000.5419

Identifier Type: -

Identifier Source: org_study_id