CABALA Diet & Health Study

NCT ID: NCT03369548

Last Updated: 2018-04-12

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

64 participants

Study Classification

INTERVENTIONAL

Study Start Date

2017-12-04

Study Completion Date

2019-12-23

Brief Summary

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During digestion of fatty foods, the liver produces a substance called bile which helps with the absorption of fat in the gut (small intestine). Some research studies have shown that friendly bacteria that live in our gut can change the makeup of bile (referred to as bile acids) leading to a lowering of blood cholesterol levels, an important risk factor for developing heart disease. This finding has been found in people who consume diets high in dietary fibers and probiotics that enhance the growth of friendly gut bacteria, and also plant rich foods high in polyphenols (such as apples). At present, very little is known about how the makeup of bile acids can regulate blood cholesterol levels and if their measurement in blood, urine or stool samples can be used as an indicator of human health.

The aim of this study is to explore how consumption of foods which enhance the growth of friendly gut bacteria (such as probiotics, prebiotics, and plant rich foods high in polyphenols) can change the makeup of bile acids after 8 weeks. Changes in the bile acids measured in blood and stool samples will then be related to markers of health, such as blood cholesterol, glucose, insulin, vascular health and inflammatory markers.

Detailed Description

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Conditions

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Healthy

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Apple/ Polyphenol

Participants will be asked to consume 2 Renetta Canada apples (with skin) and 2 placebo capsules every day for 8 weeks.

Group Type EXPERIMENTAL

Apple

Intervention Type OTHER

2 Renetta Canada apples and 2 placebo capsules/ day

Oats / Prebiotic

Participants will be asked to consume 40g jumbo rolled oats with semi-skimmed milk and 2 placebo capsules every day for 8 weeks.

Group Type EXPERIMENTAL

Oats

Intervention Type OTHER

40g jumbo rolled oats with semi-skimmed milk and 2 placebo capsules / day

Lactobacillus reuteri NCIMB 30242 / Probiotic

Participants will be asked to consume 2 probiotic capsules and 40g cornflakes with semi-skimmed milk every day for 8 weeks.

Group Type EXPERIMENTAL

Lactobacillus reuteri NCIMB 30242

Intervention Type DIETARY_SUPPLEMENT

2 probiotic capsules and 40g cornflakes with semi-skimmed milk / day.

Placebo / cornflakes

Participants will be asked to consume 40g cornflakes with semi-skimmed milk and 2 placebo capsules every day for 8 weeks.

Group Type PLACEBO_COMPARATOR

Cornflakes

Intervention Type OTHER

40g cornflakes with semi-skimmed milk and 2 placebo capsules/ day.

Interventions

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Apple

2 Renetta Canada apples and 2 placebo capsules/ day

Intervention Type OTHER

Oats

40g jumbo rolled oats with semi-skimmed milk and 2 placebo capsules / day

Intervention Type OTHER

Lactobacillus reuteri NCIMB 30242

2 probiotic capsules and 40g cornflakes with semi-skimmed milk / day.

Intervention Type DIETARY_SUPPLEMENT

Cornflakes

40g cornflakes with semi-skimmed milk and 2 placebo capsules/ day.

Intervention Type OTHER

Other Intervention Names

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Polyphenol Prebiotic Probiotic Placebo

Eligibility Criteria

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Inclusion Criteria

* Men and women
* Aged 25-70 years
* BMI: 23-32 kg/m2
* Fasting glucose \< 7 mmol/l
* Total cholesterol \< 7.5 mmol/L
* Triglycerides \< 2.3 mmol/L
* Habitual breakfast consumers
* Weight stable in the last three months

Exclusion Criteria

* Smoker
* Diabetes
* Endocrine disease
* Cardiovascular disease diagnosis
* Gastrointestinal diseases
* Pancreatic, hepatic or renal diseases
* Medications that could influence study outcomes (e.g. lipid lowering medications, anti-depressants, anticoagulants)
* Antibiotic use within the last three months
* Food allergies (e.g. gluten, dairy, apples) and intolerances (e.g. lactose)
* Alcohol or drug abuse (Drink more than 14 units of alcohol per week)
* Anemia (men:haemoglobin\<130g/ L and women \<120 g/L
* Planning or currently on a weight reducing program
* Pregnancy, planned pregnancy in the next year or lactating
* Irregular menstrual cycle
* Planning or currently on a weight reducing program
* Currently taking part or participation in other research studies within the last three months
* Recent blood donation or unwilling to refrain from donating blood during the study
* Regular consumption of probiotic or prebiotic food supplements or fiber based laxatives and unwilling stop consuming these for the duration of the study
Minimum Eligible Age

25 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University College Cork

OTHER

Sponsor Role collaborator

Fondazione Edmund Mach

OTHER

Sponsor Role collaborator

Università degli Studi dell'Insubria

OTHER

Sponsor Role collaborator

University of Reading

OTHER

Sponsor Role lead

Responsible Party

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Julie Lovegrove

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Julie A Lovegrove, Professor

Role: PRINCIPAL_INVESTIGATOR

University of Reading

Locations

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Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading

Reading, Berkshire, United Kingdom

Site Status RECRUITING

Countries

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United Kingdom

Central Contacts

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Julie A Lovegrove, Professor

Role: CONTACT

0044(0)1183786418 ext. 6418

Camilla Pedersen, PhD

Role: CONTACT

+44 (0)797 617 6090

Facility Contacts

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Julie A Lovegrove, Professor

Role: primary

0044(0)1183786418 ext. 6418

Camilla Pedersen, PhD

Role: backup

+44 (0)797 617 6090

References

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Alzoufairi S, Pushpass RG, Liu L, Lovegrove JA, Jackson KG. Impact of chronic consumption of probiotics, oats, and apples on expression of genes related to bile acids, lipid, gut peptides, and inflammation in peripheral monocular cells - findings from the CABALA study. Eur J Nutr. 2025 May 10;64(4):176. doi: 10.1007/s00394-025-03694-x.

Reference Type DERIVED
PMID: 40347281 (View on PubMed)

Other Identifiers

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17/47

Identifier Type: -

Identifier Source: org_study_id

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