The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization (IVF) Outcome of Sibling Oocytes

NCT ID: NCT02066584

Last Updated: 2014-02-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

600 participants

Study Classification

OBSERVATIONAL

Study Start Date

2014-04-30

Study Completion Date

2016-04-30

Brief Summary

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The importance of glucose in culture media on fertilization and embryo development is still controversial. Although it has been reported that glucose is essential for fertilization process and biosynthesis of membrane lipid and nucleic acids, comparatively high levels of glucose in culture media may cause developmental arrest of cleavage stage embryos. The aim of this retrospective study is to examine whether exposure of sibling oocytes to different concentrations of glucose in culture media has any further effect on IVF outcome.

Detailed Description

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This retrospective study will include data from either standard IVF or Intracytoplasmic Sperm Injection (ICSI) cycles, in which sibling oocytes were equally divided and incubated separately in one of the following pairs of culture media:

1. Universal IVF medium vs. ISM1 medium ( Origio, Medi-Cult)
2. P1 medium vs. ECM medium (Irvine).

Fertilization rate,as well as embryo cleavage rate and morphology will be evaluated.

Conditions

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Infertility

Study Design

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Observational Model Type

COHORT

Study Time Perspective

RETROSPECTIVE

Study Groups

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Universal IVF Medium

An oocyte culture medium containing 5.55 Mm glucose (Origio, Medi-Cult)

Sibling oocyte incubation

Intervention Type OTHER

ISM1 Medium

An oocyte culture medium containing 1mM glucose ((Origio, MediCult)

Sibling oocyte incubation

Intervention Type OTHER

P1 Medium

An oocyte culture medium containing no glucose (Irvine)

Sibling oocyte incubation

Intervention Type OTHER

ECM Medium

An oocyte culture medium containing 0.5mM glucose (Irvine)

Sibling oocyte incubation

Intervention Type OTHER

Interventions

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Sibling oocyte incubation

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Sibling oocytes from cycles where at least 4 oocytes were retrieved

Exclusion Criteria

* Less than 4 oocytes retrieved
* Partner has undergone testicular sperm extraction (TESE)
Minimum Eligible Age

18 Years

Maximum Eligible Age

42 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Hillel Yaffe Medical Center

OTHER_GOV

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Medeia Michaelo, PhD

Role: PRINCIPAL_INVESTIGATOR

Hellel Yaffe Medical Center

Locations

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Hillel Yaffe Medical Center

Hadera, , Israel

Site Status

Countries

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Israel

Central Contacts

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Medeia Michaeli, PhD

Role: CONTACT

972-50-6246885

Facility Contacts

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Medeia Michaeli, PhD

Role: primary

Other Identifiers

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0011-14HYMC

Identifier Type: -

Identifier Source: org_study_id

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