Fructose-induced Intestinal de Novo Lipogenesis

NCT ID: NCT01792089

Last Updated: 2019-03-25

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

24 participants

Study Classification

OBSERVATIONAL

Study Start Date

2013-02-28

Study Completion Date

2016-12-31

Brief Summary

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8 healthy volunteers, 8 pre-bypass obese subjects, 8 subjects between 12-18 months post-bypass, and 8 subjects matched to the post-bypass patients will be studied in two sub-studies Sub-study 1) after ingestion of two or three different of the following test-meals: A)cream and whey protein B)cream, whey protein and fructose + 13C6 fructose C)test meal with cream, whey protein, fructose and glucose + 13C6 fructose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood Sub-study 2) after ingestion of two or three different of the following test-meals: A)cream labelled with 13C6 palmitic acid and whey protein B))cream labelled with 13C6 palmitic acid, whey protein, fructose and glucose

Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood in sub-study 1

Their exogenous lipid absorption kinetics will be assessed by measuring 13C palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in sub-study 2

Detailed Description

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Conditions

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Obesity

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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non-obese healthy subjects

subjects with BMI\<25 and no known disease

fat+protein meal

Intervention Type OTHER

cream+ whey protein

Fat + protein + 13C fructose meal

Intervention Type OTHER

cream+ whey protein +13C fructose

Fat+protein+13C fructose+glucose meal

Intervention Type OTHER

Cream + whey protein + glucose:13C fructose mixture

13C Fat + whey protein

Intervention Type OTHER

cream labelled with 13C palmitate + whey protein

13C Fat + whey protein + glucose+ glucose meal

Intervention Type OTHER

cream labelled with 13C palmitate + whey protein + glucose:fructose mixture

post-gastric bypass

post-obese subjects 12-48 months after Roux-en-Y gastric bypass

fat+protein meal

Intervention Type OTHER

cream+ whey protein

Fat + protein + 13C fructose meal

Intervention Type OTHER

cream+ whey protein +13C fructose

13C Fat + whey protein + glucose+ glucose meal

Intervention Type OTHER

cream labelled with 13C palmitate + whey protein + glucose:fructose mixture

matched control subjects

Nonobese, healthy subjects of similar age, weight, and gender ratio, than post-bypass subjects

fat+protein meal

Intervention Type OTHER

cream+ whey protein

Fat + protein + 13C fructose meal

Intervention Type OTHER

cream+ whey protein +13C fructose

13C Fat + whey protein + glucose+ glucose meal

Intervention Type OTHER

cream labelled with 13C palmitate + whey protein + glucose:fructose mixture

Interventions

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fat+protein meal

cream+ whey protein

Intervention Type OTHER

Fat + protein + 13C fructose meal

cream+ whey protein +13C fructose

Intervention Type OTHER

Fat+protein+13C fructose+glucose meal

Cream + whey protein + glucose:13C fructose mixture

Intervention Type OTHER

13C Fat + whey protein

cream labelled with 13C palmitate + whey protein

Intervention Type OTHER

13C Fat + whey protein + glucose+ glucose meal

cream labelled with 13C palmitate + whey protein + glucose:fructose mixture

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* age 18-50 years
* sex male or female
* less than 2 kg body weight change over the past 3 months subjects

Exclusion Criteria

* antidiabetic and hypolipemic drugs
* alcohol consumption \>20g/day
* severe eating disorders
* severe psychological problems
* vegetarian diet or other specific diet
* consumption of illicit substances
* pregnancy
* weight gain or weight loss \> 1 kg in the last 3 months
Minimum Eligible Age

25 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Lausanne

OTHER

Sponsor Role lead

Responsible Party

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Luc Tappy, MD

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Centre Hospitalier Universitaire Vaudois

Lausanne, Canton of Vaud, Switzerland

Site Status

Countries

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Switzerland

References

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Theytaz F, de Giorgi S, Hodson L, Stefanoni N, Rey V, Schneiter P, Giusti V, Tappy L. Metabolic fate of fructose ingested with and without glucose in a mixed meal. Nutrients. 2014 Jul 15;6(7):2632-49. doi: 10.3390/nu6072632.

Reference Type DERIVED
PMID: 25029210 (View on PubMed)

Other Identifiers

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193/11

Identifier Type: -

Identifier Source: org_study_id

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