Evaluate Bacteriophage as a Useful Immunogen in Patients With Primary Immune Deficiency Diseases (PIDD)

NCT ID: NCT01617122

Last Updated: 2012-07-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

200 participants

Study Classification

INTERVENTIONAL

Study Start Date

1995-10-31

Study Completion Date

2015-12-31

Brief Summary

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This protocol is designed to ascertain whether the bacteriophage 0X174 neoantigen is safe and effective as an antigen used in the evaluation of primary and secondary immune responses. Bacteriophage 0X174 is given intravenously 2 billion PFU/Kg of body weight; small blood specimens of 3-5 ml (about 1 teaspoon) are collected after 15 minutes, 7 days, 14 days, and 28 days. Blood is collected at intervals following the administration of the bacteriophage and the number of phage/ml is determined by the agar overlay method using suspension of E. coli C and serially diluted patient's serum. Phage-specific IgG and IgM are measured by neutralization assay. Capacity of switch from IgM to IgG is determined.

Detailed Description

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Conditions

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Primary Immune Deficiency Diseases

Keywords

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PID PIDD antibody deficiency CVID recurrent infections

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Bacteriophage

Subjects receive bacteriophage vaccinations and blood draws

Group Type EXPERIMENTAL

Bacteriophage OX174

Intervention Type BIOLOGICAL

Give first dose of bacteriophage (2 x 109 PFU/kg body weight (0.02 ml/kg)) at visit one. Obtain labs prior to vaccine, 15 minutes, 1 week, 2 weeks, and 4 weeks after vaccine. 6 weeks after 1st vaccine, obtain blood, give 2nd dose of bacteriophage, obtain labs 15 minutes, 1 week, 2 weeks, and 4 weeks after vaccine. Selected patients may receive a tertiary vaccine.

Interventions

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Bacteriophage OX174

Give first dose of bacteriophage (2 x 109 PFU/kg body weight (0.02 ml/kg)) at visit one. Obtain labs prior to vaccine, 15 minutes, 1 week, 2 weeks, and 4 weeks after vaccine. 6 weeks after 1st vaccine, obtain blood, give 2nd dose of bacteriophage, obtain labs 15 minutes, 1 week, 2 weeks, and 4 weeks after vaccine. Selected patients may receive a tertiary vaccine.

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

1. subject/parent or guardian willing to sign consent and adhere to study schedule
2. known or suspected primary immune deficiency

Exclusion Criteria

1. pregnancy
2. breastfeeding
3. unwilling to sign consent or adhere to study schedule
4. \< 2 yrs of age or \> 85 yrs of age
5. previous reaction to vaccine
Minimum Eligible Age

2 Years

Maximum Eligible Age

85 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of South Florida

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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John W Sleasman, MD

Role: PRINCIPAL_INVESTIGATOR

University of South Florida

Locations

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University of South Florida

St. Petersburg, Florida, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Carla Duff, RN BSN CCRP

Role: CONTACT

Phone: 727-553-3515

Email: [email protected]

Amy Kramer

Role: CONTACT

Phone: 727-553-1258

Email: [email protected]

Facility Contacts

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Carla Duff, RN BSN CCRP

Role: primary

Amy Kramer

Role: backup

Other Identifiers

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ACH 03-0120

Identifier Type: -

Identifier Source: org_study_id